help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pillebout, E.
Right arrow Articles by Terzi, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pillebout, E.
Right arrow Articles by Terzi, F.
(American Journal of Pathology. 2001;159:547-560.)
© 2001 American Society for Investigative Pathology

Regular Articles

Proliferation and Remodeling of the Peritubular Microcirculation after Nephron Reduction

Association with the Progression of Renal Lesions

Evangéline Pillebout*, Martine Burtin*, Hai T. Yuan{dagger}, Pascale Briand{ddagger}, Adrian S. Woolf{dagger}, Gérard Friedlander* and Fabiola Terzi*

From INSERM U426,

* Faculté de Médecine Xavier Bichat, Université Paris, Paris, France; INSERM U380,

{ddagger} Institut Cochin de Génétique Moléculaire, Paris, France; and the Nephro-Urology Unit,

{dagger} Institute of Child Health, University College London, London, United Kingdom

Little is known about the serial changes that might occur in renal capillaries after reduction of renal mass. In the current study, our aim was to document potential alterations in the morphology and proliferation of the renal cortical peritubular microcirculation at specific time points (7 and 60 days) after experimental 75% surgical nephron reduction using two strains of mice that we here demonstrate react differently to the same initial insult: one strain (C57BL6xDBA2/F1 mice) undergoes compensatory growth alone, whereas the other (FVB/N mice) additionally develops severe tubulo-interstitial lesions. Our data demonstrate that significant remodeling and proliferation occur in renal cortical peritubular capillaries after experimental nephron reduction, as assessed by microangiography using infusion of fluorescein isothiocyanate-labeled dextran, expression of the endothelial markers CD34 and Tie-2, and co-expression of CD34 and proliferating cell nuclear antigen, a surrogate marker of cell proliferation. This was accompanied by an increase of renal vascular endothelial growth factor protein levels and a change in distribution of this protein within the kidney itself. Moreover, most of these responses were accentuated in FVB/N mice in the presence of progressive renal disease and positively correlated with tubular epithelial cell proliferation. Hence, we have made three significant novel observations that illuminate the complex pathophysiology of chronic kidney damage after nephron reduction: 1) cortical peritubular capillaries grow by proliferation and remodeling, 2) vascular endothelial growth factor expression is altered, and 3) the development of tubulo-interstitial disease is genetically determined.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Doucet, S. Milin, F. Favreau, T. Desurmont, E. Manguy, W. Hebrard, Y. Yamamoto, G. Mauco, M. Eugene, V. Papadopoulos, et al.
A p38 mitogen-activated protein kinase inhibitor protects against renal damage in a non-heart-beating donor model
Am J Physiol Renal Physiol, July 1, 2008; 295(1): F179 - F191.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
N. Alenina, P. Xu, B. Rentzsch, E. L. Patkin, and M. Bader
Genetically altered animal models for Mas and angiotensin-(1-7)
Exp Physiol, May 1, 2008; 93(5): 528 - 537.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. E. Burt, M. S. Forbes, B. A. Thornhill, S. C. Kiley, and R. L. Chevalier
Renal vascular endothelial growth factor in neonatal obstructive nephropathy. I. Endogenous VEGF
Am J Physiol Renal Physiol, January 1, 2007; 292(1): F158 - F167.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. A. Long, W. Mu, K. L. Price, C. Roncal, G. F. Schreiner, A. S. Woolf, and R. J. Johnson
Vascular endothelial growth factor administration does not improve microvascular disease in the salt-dependent phase of post-angiotensin II hypertension
Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1248 - F1254.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. D. Plotkin and M. S. Goligorsky
Mesenchymal cells from adult kidney support angiogenesis and differentiate into multiple interstitial cell types including erythropoietin-producing fibroblasts
Am J Physiol Renal Physiol, October 1, 2006; 291(4): F902 - F912.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. K. Kairaitis, Y. Wang, M. Gassmann, Y.-C. Tay, and D. C. H. Harris
HIF-1{alpha} expression follows microvascular loss in advanced murine adriamycin nephrosis
Am J Physiol Renal Physiol, January 1, 2005; 288(1): F198 - F206.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. E. Pitera, A. S. Woolf, N. W. Gale, G. D. Yancopoulos, and H. T. Yuan
Dysmorphogenesis of Kidney Cortical Peritubular Capillaries in Angiopoietin-2-Deficient Mice
Am. J. Pathol., December 1, 2004; 165(6): 1895 - 1906.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. Manotham, T. Tanaka, M. Matsumoto, T. Ohse, T. Miyata, R. Inagi, K. Kurokawa, T. Fujita, and M. Nangaku
Evidence of Tubular Hypoxia in the Early Phase in the Remnant Kidney Model
J. Am. Soc. Nephrol., May 1, 2004; 15(5): 1277 - 1288.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H.-T. Yuan, X.-Z. Li, J. E. Pitera, D. A. Long, and A. S. Woolf
Peritubular Capillary Loss after Mouse Acute Nephrotoxicity Correlates with Down-Regulation of Vascular Endothelial Growth Factor-A and Hypoxia-Inducible Factor-1{alpha}
Am. J. Pathol., December 1, 2003; 163(6): 2289 - 2301.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
K. Matsui, K. Nagy-Bojarsky, P. Laakkonen, S. Krieger, K. Mechtler, S. Uchida, S. Geleff, D.-H. Kang, R. J. Johnson, and D. Kerjaschki
Lymphatic Microvessels in the Rat Remnant Kidney Model of Renal Fibrosis: Aminopeptidase P and Podoplanin Are Discriminatory Markers for Endothelial Cells of Blood and Lymphatic Vessels
J. Am. Soc. Nephrol., August 1, 2003; 14(8): 1981 - 1989.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
B.-S. Kim, J. Chen, T. Weinstein, E. Noiri, and M. S. Goligorsky
VEGF Expression in Hypoxia and Hyperglycemia: Reciprocal Effect on Branching Angiogenesis in Epithelial-Endothelial Co-Cultures
J. Am. Soc. Nephrol., August 1, 2002; 13(8): 2027 - 2036.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. Flyvbjerg, B. F. Schrijvers, A. S. De Vriese, R. G. Tilton, and R. Rasch
Compensatory glomerular growth after unilateral nephrectomy is VEGF dependent
Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E362 - E366.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Ohashi, A. Shimizu, Y. Masuda, H. Kitamura, M. Ishizaki, Y. Sugisaki, and N. Yamanaka
Peritubular Capillary Regression during the Progression of Experimental Obstructive Nephropathy
J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1795 - 1805.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
D.-H. Kang, J. Kanellis, C. Hugo, L. Truong, S. Anderson, D. Kerjaschki, G. F. Schreiner, and R. J. Johnson
Role of the Microvascular Endothelium in Progressive Renal Disease
J. Am. Soc. Nephrol., March 1, 2002; 13(3): 806 - 816.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
D. A. Long, A. S. Woolf, T. Suda, and H. T. Yuan
Increased Renal Angiopoietin-1 Expression in Folic Acid-Induced Nephrotoxicity in Mice
J. Am. Soc. Nephrol., December 1, 2001; 12(12): 2721 - 2731.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society for Investigative Pathology.