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(American Journal of Pathology. 2001;159:937-943.)
© 2001 American Society for Investigative Pathology

Regular Articles

The Fluorescent Congo Red Derivative, (Trans, Trans)-1-Bromo-2,5-Bis-(3-Hydroxycarbonyl-4-Hydroxy)Styrylbenzene (BSB), Labels Diverse ß-Pleated Sheet Structures in Postmortem Human Neurodegenerative Disease Brains

Marie L. Schmidt*, Theresa Schuck*, Shelly Sheridan*, Mei-Ping Kung{dagger}, Hank Kung{ddagger}, Zhi-Ping Zhuang{ddagger}, Catherine Bergeron§, Jacque S. Lamarche, Daniel Skovronsky*, Benoit I. Giasson*, Virginia M.-Y. Lee* and John Q. Trojanowski*

From the Departments of Pathology and Laboratory Medicine,*
Radiology,{dagger}
and Pharmacology,{ddagger}
the Center for Neurodegenerative Disease Research, University of Pennsylvania, Philadelphia, Pennsylvania; the University of Toronto,§
Toronto, Ontario, Canada; and the Department of Pathology,
University of Sherbrooke, Sherbrooke, Quebec, Canada

A novel Congo red-derived fluorescent probe (trans, trans),-1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene (BSB) that binds to amyloid plaques of postmortem Alzheimer’s disease brains and in transgenic mouse brains in vivo was designed as a prototype imaging agent for Alzheimer’s disease. In the current study, we used BSB to probe postmortem tissues from patients with various neurodegenerative diseases with diagnostic lesions characterized by fibrillar intra- or extracellular lesions and compared these results with standard histochemical dyes such as thioflavin S and immunohistochemical stains specific for the same lesions. These data show that BSB binds not only to extracellular amyloid ß protein, but also many intracellular lesions composed of abnormal tau and synuclein proteins and suggests that radioiodinated BSB derivatives or related ligands may be useful imaging agents to monitor diverse amyloids in vivo.






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Copyright © 2001 by the American Society for Investigative Pathology.