help button home button Am J Pathol sign up for etoc
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klekotka, P. A.
Right arrow Articles by Zutter, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klekotka, P. A.
Right arrow Articles by Zutter, M. M.
(American Journal of Pathology. 2001;159:983-992.)
© 2001 American Society for Investigative Pathology

Regular Articles

Mammary Epithelial Cell-Cycle Progression via the {alpha}2ß1 Integrin

Unique and Synergistic Roles of the {alpha}2 Cytoplasmic Domain

Paul A. Klekotka*, Samuel A. Santoro*, Alan Ho{dagger}, Steven F. Dowdy{dagger} and Mary M. Zutter*

From the Department of Pathology and Immunology,*
Washington University School of Medicine, St. Louis, Missouri; and the Howard Hughes Medical Institute,{dagger}
Chevy Chase, Maryland

The {alpha}2ß1 integrin supports cell-cycle progression of mammary epithelial cells adherent to type I collagen matrices. Integrin collagen receptors containing the {alpha}2 cytoplasmic domain stimulated expression of cyclin E and cyclin-dependent kinase (cdk)2, resulting in cyclin E/cdk2 activation in the absence of growth factors other than insulin. Integrin collagen receptors in which the {alpha}2 cytoplasmic domain was replaced by the {alpha}1 cytoplasmic domain or an {alpha}2 subunit cytoplasmic domain truncated after the GFFKR sequence failed to stimulate cyclin E/cdk2 activation or entry into S phase in the absence of growth factors. Although overexpression of cyclins D or E or cdk2 in cells expressing the integrin collagen receptor with the {alpha}1-integrin cytoplasmic domain did not restore G1 progression when mammary epithelial cells adhered to type I collagen, co-expression of cyclin E and cdk2 did rescue the ability of the transfectants to enter S phase. Activation of cyclin E/cdk2 complex by mammary epithelial cells required synergy between adhesion mediated by an integrin collagen receptor containing the {alpha}2-integrin subunit cytoplasmic domain and the insulin receptor.




This article has been cited by other articles:


Home page
 BloodHome page
Z. Zhang, N. E. Ramirez, T. E. Yankeelov, Z. Li, L. E. Ford, Y. Qi, A. Pozzi, and M. M. Zutter
{alpha}2{beta}1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner
Blood, February 15, 2008; 111(4): 1980 - 1988.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Pellinen and J. Ivaska
Integrin traffic.
J. Cell Sci., September 15, 2006; 119(Pt 18): 3723 - 3731.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. J. Wagers and I. L. Weissman
Differential Expression of {alpha}2 Integrin Separates Long-Term and Short-Term Reconstituting Lin-/loThy1.1loc-kit+ Sca-1+ Hematopoietic Stem Cells
Stem Cells, April 1, 2006; 24(4): 1087 - 1094.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Sanz, R.-Q. Chen, A. Perez, R. Hilario, P. Juarez, C. Marcinkiewicz, D. Monleon, B. Celda, Y.-L. Xiong, E. Perez-Paya, et al.
cDNA Cloning and Functional Expression of Jerdostatin, a Novel RTS-disintegrin from Trimeresurus jerdonii and a Specific Antagonist of the {alpha}1{beta}1 Integrin
J. Biol. Chem., December 9, 2005; 280(49): 40714 - 40722.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. A. Sanders and M. D. Basson
Collagen IV regulates Caco-2 migration and ERK activation via {alpha}1{beta}1- and {alpha}2{beta}1-integrin-dependent Src kinase activation
Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G547 - G557.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. Keay, F. Seillier-Moiseiwitsch, C.-O. Zhang, T. C. Chai, and J. Zhang
Changes in human bladder epithelial cell gene expression associated with interstitial cystitis or antiproliferative factor treatment
Physiol Genomics, July 7, 2003; 14(2): 107 - 115.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. Chen, T. G. Diacovo, D. G. Grenache, S. A. Santoro, and M. M. Zutter
The {alpha}2 Integrin Subunit-Deficient Mouse : A Multifaceted Phenotype Including Defects of Branching Morphogenesis and Hemostasis
Am. J. Pathol., July 1, 2002; 161(1): 337 - 344.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society for Investigative Pathology.