help button home button Am J Pathol ASIP WHAT IS IT?
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vecchione, A.
Right arrow Articles by Baffa, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vecchione, A.
Right arrow Articles by Baffa, R.
(American Journal of Pathology. 2002;160:1345-1352.)
© 2002 American Society for Investigative Pathology

Regular Articles

FEZ1/LZTS1 Is Down-Regulated in High-Grade Bladder Cancer, and Its Restoration Suppresses Tumorigenicity in Transitional Cell Carcinoma Cells

Andrea Vecchione*§, Hideshi Ishii{dagger}, Gustavo Baldassarre{dagger}, Pierfrancesco Bassi{ddagger}, Francesco Trapasso{dagger}, Hansjuerg Alder{dagger}, Francesco Pagano{ddagger}, Leonard G. Gomella*, Carlo M. Croce{dagger} and Raffaele Baffa*

From the Departments of Urology* andMicrobiology/Immunology,{dagger} Kimmel Cancer Center,Thomas Jefferson University, Philadelphia, Pennsylvania; the Departmentof Urology,{ddagger} University of Padova, Padua,Italy; and the Division of Pathology,§ IIUniversity of Rome "La Sapienza", Ospedale Santo Andrea,Rome, Italy

FEZ1/LZTS1 is a tumor suppressor gene that maps to chromosome 8p22, a chromosomal region frequently deleted in many human malignancies, including transitional cell carcinoma (TCC) of the urinary bladder. FEZ1/LZTS1 alterations have been reported in esophageal, breast, prostate, and gastric carcinomas. Fez1 expression was studied in five TCC-derived cancer cell lines by Western blot analysis and in 60 primary TCCs of the urinary bladder by immunohistochemistry. Fez1 protein was absent or reduced in four of five cell lines and in 37 of 60 primary TCC examined. We also restored Fez1 protein expression in human SW780 TCC-derived cells lacking endogenous Fez1 protein to study the effects of Fez1 expression on cell proliferation, cell kinetics, and tumorigenicity in BALB/c nude mice. In vitro transduction of SW780 Fez1-negative cell, with Ad-FEZ1, inhibited cell growth, altered cell cycle progression, and suppressed subcutaneous tumor growth in nude mice. These results suggest that FEZ1/LZTS1 gene plays a role in the development of TCC of the urinary bladder by acting as a bona fide tumor suppressor gene both in vitro and in vivo.




This article has been cited by other articles:


Home page
 CarcinogenesisHome page
R. Baffa, M. Fassan, C. Sevignani, A. Vecchione, H. Ishii, E. Giarnieri, R. V. Iozzo, L. G. Gomella, and C. M. Croce
Fez1/Lzts1-deficient mice are more susceptible to N-butyl-N-(4-hydroxybutil) nitrosamine (BBN) carcinogenesis
Carcinogenesis, April 1, 2008; 29(4): 846 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. Nonaka, A. Fabbri, L. Roz, L. Mariani, A. Vecchione, G. W. Moore, L. Tavecchio, C. M. Croce, and G. Sozzi
Reduced FEZ1/LZTS1 Expression and Outcome Prediction in Lung Cancer
Cancer Res., February 15, 2005; 65(4): 1207 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Vecchione, C. Sevignani, E. Giarnieri, N. Zanesi, H. Ishii, R. Cesari, L. Y. Y. Fong, L. G. Gomella, C. M. Croce, and R. Baffa
Inactivation of the FHIT Gene Favors Bladder Cancer Development
Clin. Cancer Res., November 15, 2004; 10(22): 7607 - 7612.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society for Investigative Pathology.