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(American Journal of Pathology. 2002;161:291-300.)
© 2002 American Society for Investigative Pathology

Regular Articles

Complicated Mechanisms of Class II Transactivator Transcription Deficiency in Small Cell Lung Cancer and Neuroblastoma

Takuya Yazawa^*, Takaaki Ito^*, Hiroshi Kamma, Takehisa Suzuki^*, Koji Okudela^*, Hiroyuki Hayashi^*, Hisashi Horiguchi, Takesaburo Ogata, Hideaki Mitsui^*, Masaichi Ikeda^* and Hitoshi Kitamura^*

From the Department of Pathology,^*Yokohama City University School of Medicine, Kanagawa; the Department of Pathology,Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki; and the Department of Pathology,Center for Medical Sciences, Ibaraki Prefectural University of Health Sciences, Ibaraki, Japan

Small cell lung cancer (SCLC) and neuroblastoma (NB), the most aggressive adult and infant neuroendocrine cancers, respectively, are immunologically characterized by a severe reduction in major histocompatibility complex (MHC) that is indispensable for anti-tumor immunity. We had reported that the severe reduction of MHC in SCLC was caused by a deficient interferon (IFN)--inducible expression of class II transactivator (CIITA) that is known as a very important transcription factor for IFN--inducible class II and class I MHC expression (Yazawa T, Kamma H, Fujiwara M, Matsui M, Horiguchi H, Satoh H, Fujimoto M, Yokohama K, Ogata T: Lack of class II transactivator causes severe deficiency of HLA-DR expression in small cell lung cancer. J Pathol 1999, 187:191–199). Here, we demonstrate that the reduction of MHC in NB was also caused by a deficient IFN--inducible expression of CIITA and that the deficiency in SCLC and NB was caused by similar mechanisms. Human achaete-scute complex homologue (HASH)-1, L-myc, and N-myc, which are specifically overexpressed in SCLC and NB, bound to the E-box in CIITA promoter IV and reduced the transcriptional activity. Anti-sense oligonucleotide experiments revealed that overexpressed L-myc and N-myc lie upstream in the regulatory pathway of HASH-1 expression. The expression of HASH-1 was also up-regulated by IFN-. Our results suggest that SCLC and NB have complicated mechanisms of IFN--inducible CIITA transcription deficiency through the overexpressed HASH-1, L-myc, and N-myc. These complicated mechanisms may play an important role in the escape from anti-tumor immunity.

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