| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
B Activation and Interleukin-8 Production in Human Intestinal Epithelial Cells
From the Department of Surgery, University of Medicine and Dentistry-New Jersey Medical School, Newark, New Jersey
Inflammatory bowel disease of the colon is associated with a high osmolarity of colonic contents. We hypothesized that this hyperosmolarity may contribute to colonic inflammation by stimulating the proinflammatory activity of intestinal epithelial cells (IECs). The human IEC lines HT-29 and Caco-2 were used to study the effect of hyperosmolarity on the IEC inflammatory response. Exposure of IECs to hyperosmolarity triggered expression of the proinflammatory chemokine interleukin (IL)-8 both at the secreted protein and mRNA levels. In addition, hyperosmotic stimulation induced the release of another chemokine, GRO-
. These effects were because of activation of the transcription factor, nuclear factor (NF)-
B, because hyperosmolarity stimulated both NF-B DNA binding and NF-B-dependent transcriptional activity. Hyperosmolarity activated both p38 and p42/44 mitogen-activated protein kinases, which effect contributed to hyperosmolarity-stimulated IL-8 production, because p38 and p42/44 inhibition prevented the hyperosmolarity-induced increase in IL-8 production. In addition, the proinflammatory effects of hyperosmolarity were, in a large part, mediated by activation of Na+/H+ exchangers, because selective blockade of Na+/H+ exchangers prevented the hyperosmolarity-induced IEC inflammatory response. In summary, hyperosmolarity stimulates IEC IL-8 production, which effect may contribute to the maintenance of inflammation in inflammatory bowel disease.
This article has been cited by other articles:
 |
|
 |
|
  M. B. Burg, J. D. Ferraris, and N. I. Dmitrieva Cellular Response to Hyperosmotic Stresses Physiol Rev, October 1, 2007; 87(4): 1441 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Borthakur, S. Bhattacharyya, P. K. Dudeja, and J. K. Tobacman Carrageenan induces interleukin-8 production through distinct Bcl10 pathway in normal human colonic epithelial cells Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G829 - G838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Xu, W. Tian, J. N. Lindsley, T. T. Oyama, J. M. Capasso, C. J. Rivard, H. T. Cohen, S. M. Bagnasco, S. Anderson, and D. M. Cohen EphA2: expression in the renal medulla and regulation by hypertonicity and urea stress in vitro and in vivo Am J Physiol Renal Physiol, April 1, 2005; 288(4): F855 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sheikh-Hamad and M. C. Gustin MAP kinases and the adaptive response to hypertonicity: functional preservation from yeast to mammals Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1102 - F1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Stambe, D. J. Nikolic-Paterson, P. A. Hill, J. Dowling, and R. C. Atkins p38 Mitogen-Activated Protein Kinase Activation and Cell Localization in Human Glomerulonephritis: Correlation with Renal Injury J. Am. Soc. Nephrol., February 1, 2004; 15(2): 326 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. H. Nemeth, H. R. Wong, K. Odoms, E. A. Deitch, C. Szabo, E. S. Vizi, and G. Hasko Proteasome Inhibitors Induce Inhibitory {kappa}B (I{kappa}B) Kinase Activation, I{kappa}B{alpha} Degradation, and Nuclear Factor {kappa}B Activation in HT-29 Cells Mol. Pharmacol., February 1, 2004; 65(2): 342 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kumar, A. J. Knox, and A. M. Boriek CCAAT/Enhancer-binding Protein and Activator Protein-1 Transcription Factors Regulate the Expression of Interleukin-8 through the Mitogen-activated Protein Kinase Pathways in Response to Mechanical Stretch of Human Airway Smooth Muscle Cells J. Biol. Chem., May 23, 2003; 278(21): 18868 - 18876. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
