This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Related articles in Am J Pathol Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lambert, V. Articles by Rakic, J.-M. Search for Related Content PubMed PubMed Citation Articles by Lambert, V. Articles by Rakic, J.-M.

(American Journal of Pathology. 2002;161:1247-1253.)
© 2002 American Society for Investigative Pathology

Regular Articles

Matrix Metalloproteinase-9 Contributes to Choroidal Neovascularization

Vincent Lambert^*, Carine Munaut^*, Maud Jost^*, Agnès Noël^*, Zena Werb, Jean-Michel Foidart^* and Jean-Marie Rakic

From the Laboratory of Tumor and Development Biology,^* University of Liège, Liège, Belgium; the Department of Anatomy, University of California at San Francisco, San Francisco, California; and the Department of Ophthalmology, University Hospital, Sart-Tilman, Liège, Belgium

Age-related macular degeneration (AMD) is the primary cause of irreversible photoreceptors loss in adult patients and current therapies are limited. Increased levels of matrix metalloproteinases (MMPs) have been documented in neovascularization of severe ocular pathologies such as AMD and proliferative diabetic retinopathy. We report here that MMP-9 (gelatinase B) expression is induced and temporally regulated in the course of experimental choroidal neovascularization. We used transgenic mice expressing ß-galactosidase reporter gene under the dependence of MMP-9 promoter and RT-PCR analysis on choroidal neovascular structures microdissected from serial sections by laser pressure catapulting to show that MMP-9 expression is up-regulated concomitantly with the appearance of inflammatory cells in the subretinal lesion. In mice deficient in MMP-9 expression the development of choroidal neovascularization induced by laser photocoagulation still occurred, but at a reduced level.

Related articles in Am J Pathol:

This Month in AJP

Am J Pathol 2002 161: 1099-1100. [Full Text]  

This article has been cited by other articles:

				M. Hollborn, C. Stathopoulos, A. Steffen, P. Wiedemann, L. Kohen, and A. Bringmann Positive Feedback Regulation between MMP-9 and VEGF in Human RPE Cells Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4360 - 4367. [Abstract] [Full Text] [PDF]

				O. Tatar, A. Adam, K. Shinoda, T. Eckert, G. B Scharioth, M. Klein, E. Yoeruek, K. U. Bartz-Schmidt, and S. Grisanti Matrix metalloproteinases in human choroidal neovascular membranes excised following verteporfin photodynamic therapy Br. J. Ophthalmol., September 1, 2007; 91(9): 1183 - 1189. [Abstract] [Full Text] [PDF]

				Z. B. Levashova, N. Sharma, O. A. Timofeeva, J. S. Dome, and A. O. Perantoni ELR+-CXC Chemokines and Their Receptors in Early Metanephric Development J. Am. Soc. Nephrol., August 1, 2007; 18(8): 2359 - 2370. [Abstract] [Full Text] [PDF]

				S. Sivaprasad, N. V. Chong, and T. A. Bailey Serum Elastin-Derived Peptides in Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., September 1, 2005; 46(9): 3046 - 3051. [Abstract] [Full Text] [PDF]

				D. E. Sullivan, M. Ferris, D. Pociask, and A. R. Brody Tumor Necrosis Factor-{alpha} Induces Transforming Growth Factor-{beta}1 Expression in Lung Fibroblasts Through the Extracellular Signal-Regulated Kinase Pathway Am. J. Respir. Cell Mol. Biol., April 1, 2005; 32(4): 342 - 349. [Abstract] [Full Text] [PDF]

				F. J. Descamps, E. Martens, P. Proost, S. Starckx, P. E. Van den Steen, J. Van Damme, and G. Opdenakker Gelatinase B/matrix metalloproteinase-9 provokes cataract by cleaving lens {beta}B1 crystallin FASEB J, January 1, 2005; 19(1): 29 - 35. [Abstract] [Full Text] [PDF]

				P. Baluk, W. W. Raymond, E. Ator, L. M. Coussens, D. M. McDonald, and G. H. Caughey Matrix metalloproteinase-2 and -9 expression increases in Mycoplasma-infected airways but is not required for microvascular remodeling Am J Physiol Lung Cell Mol Physiol, August 1, 2004; 287(2): L307 - L317. [Abstract] [Full Text] [PDF]

				C. Johnson, H.-J. Sung, S. M. Lessner, M. E. Fini, and Z. S. Galis Matrix Metalloproteinase-9 Is Required for Adequate Angiogenic Revascularization of Ischemic Tissues: Potential Role in Capillary Branching Circ. Res., February 6, 2004; 94(2): 262 - 268. [Abstract] [Full Text] [PDF]

				M. H. Criswell, T. A. Ciulla, T. E. Hill, W. Small, R. P. Danis, W. J. Snyder, L. A. Lowseth, and D. L. Carson The Squirrel Monkey: Characterization of a New-World Primate Model of Experimental Choroidal Neovascularization and Comparison with the Macaque Invest. Ophthalmol. Vis. Sci., February 1, 2004; 45(2): 625 - 634. [Abstract] [Full Text] [PDF]

				K. Ohno-Matsui, T. Uetama, T. Yoshida, M. Hayano, T. Itoh, I. Morita, and M. Mochizuki Reduced Retinal Angiogenesis in MMP-2-Deficient Mice Invest. Ophthalmol. Vis. Sci., December 1, 2003; 44(12): 5370 - 5375. [Abstract] [Full Text] [PDF]

				J.-M. Rakic, V. Lambert, L. Devy, A. Luttun, P. Carmeliet, C. Claes, L. Nguyen, J.-M. Foidart, A. Noel, and C. Munaut Placental Growth Factor, a Member of the VEGF Family, Contributes to the Development of Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 3186 - 3193. [Abstract] [Full Text] [PDF]

				V. Lambert, C. Munaut, P. Carmeliet, R. D. Gerard, P. J. Declerck, A. Gils, C. Claes, J.-M. Foidart, A. Noel, and J.-M. Rakic Dose-Dependent Modulation of Choroidal Neovascularization by Plasminogen Activator Inhibitor Type I: Implications for Clinical Trials Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2791 - 2797. [Abstract] [Full Text] [PDF]

				J.-M. Rakic, V. Lambert, C. Munaut, K. Bajou, K. Peyrollier, M.-L. Alvarez-Gonzalez, P. Carmeliet, J.-M. Foidart, and A. Noel Mice without uPA, tPA, or Plasminogen Genes Are Resistant to Experimental Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1732 - 1739. [Abstract] [Full Text] [PDF]