help button home button Am J Pathol PCR Enhanced. PCRboost from Biomatrica
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Genescà, M.
Right arrow Articles by Hotter, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Genescà, M.
Right arrow Articles by Hotter, G.
(American Journal of Pathology. 2002;161:1839-1847.)
© 2002 American Society for Investigative Pathology

Role of Changes in Tissular Nucleotides on the Development of Apoptosis during Ischemia/Reperfusion in Rat Small Bowel

Meritxell Genescà*, Anna Sola*, Rosa Miquel{dagger}, Felip Pi*, Carme Xaus*, Vicente Alfaro{ddagger} and Georgina Hotter*

From the Department of Medical Bioanalysis,* Instituto de Investigaciones de Barcelona-Consejo Superior de Investigaciones Cientificas, Institut d’ Investigacions Biomèdiques August Pi i Sunyer, Barcelona; the Department of Pathology,{dagger} Hospital Clínic of Barcelona, Barcelona; and the Department of Physiology,{ddagger} Faculty of Biology, University of Barcelona, Barcelona, Spain

The aim of this study was to evaluate whether xanthine and adenosine, substances modified proportionally to the duration of ischemia, can determine cell demise (apoptosis/necrosis) during intestinal ischemia/reperfusion (I/R) and to determine the role of nitric oxide (NO) during this process. The following experimental groups were studied: I, cold ischemia; I+X, effect of xanthine; I+T, effect of adenosine (blocking its receptor by theophylline); I+A, effect of excess adenosine; I+T+X, effect of xanthine alone, and I+T+ spermine NONOate (NONOs), I+A+NONOs, I+X+NONOs, role of NO. DNA fragmentation, xanthine/adenosine levels, caspase-3 activity, NO generation, and histological analysis were measured in tissue samples. The rats treated with xanthine or adenosine showed increased levels of caspase-3 activity and DNA fragmentation. In contrast, theophylline-treated rats showed decreased levels of DNA fragmentation and tended to show lower mean values of caspase-3 activity. Administration of xanthine or NONOs to theophylline-treated rats reversed these effects. The results of histological evaluation were in agreement with these previous results. In conclusion, the present study indicates that xanthine and adenosine induced an apoptotic response during cold ischemic preservation of rat small intestine. In particular, the action of adenosine on apoptotic events was mediated by NO. We consider that identification of the role of these factors may help to define the best conditions of tissue preservation before intestinal transplantation.




This article has been cited by other articles:


Home page
 JPEN J Parenter Enteral NutrHome page
R. Yang, L. Martin-Hawver, C. Woodall, A. Thomas, N. Qureshi, D. Morrison, and C. Van Way III
Administration of Glutamine After Hemorrhagic Shock Restores Cellular Energy, Reduces Cell Apoptosis and Damage, and Increases Survival
JPEN J Parenter Enteral Nutr, March 1, 2007; 31(2): 94 - 100.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society for Investigative Pathology.