help button home button Am J Pathol Epitomics, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mahoney, E.
Right arrow Articles by Albina, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mahoney, E.
Right arrow Articles by Albina, J.
(American Journal of Pathology. 2002;161:2143-2152.)
© 2002 American Society for Investigative Pathology

Regular Articles

Bacterial Colonization and the Expression of Inducible Nitric Oxide Synthase in Murine Wounds

Eric Mahoney*, Jonathan Reichner*, Leslie Robinson Bostom{dagger}, Balduino Mastrofrancesco*, William Henry* and Jorge Albina*

From the Department of Surgery,* Division of Surgical Research, and the Department of Dermatology,{dagger} Brown Medical School/Rhode Island Hospital, Providence, Rhode Island

The expression of inducible nitric oxide synthase (iNOS) in two different murine wound models was investigated. Animals were subjected to either full-thickness linear skin incision with subcutaneous implantation of sterile polyvinyl alcohol sponges, or to 1.5 x 1.5-cm dorsal skin excision. Reverse transcriptase-polymerase chain reaction detected iNOS mRNA in all cell samples retrieved from the sponges. Immunoblotting of lysates of inflammatory cells harvested from the sponges failed to detect iNOS protein, and immunohistochemistry of the incisional wound was mildly positive. Inflammatory cells of excisional wounds stained strongly positive for iNOS. Cutaneous wounds were found to be colonized with Staphylococcus aureus. The detection of iNOS in cells from sponges inoculated in vivo with heat-killed bacteria and the reduction of immunohistochemical signal for iNOS in excisional wounds of animals treated with antibiotics support a role of bacteria in the induction of iNOS in wounds. The expression of iNOS in excisional wounds requires interferon-{gamma} and functional lymphocytes because interferon-{gamma} knockout and SCID-Beige mice exhibited attenuated iNOS staining in excisional wounds. The expression of iNOS in the inflammatory cells of murine wounds is a response to bacterial colonization and not part of the normal repair process elicited by sterile tissue injury.




This article has been cited by other articles:


Home page
 J. Leukoc. Biol.Home page
J. M. Daley, S. K. Brancato, A. A. Thomay, J. S. Reichner, and J. E. Albina
The phenotype of murine wound macrophages
J. Leukoc. Biol., January 1, 2010; 87(1): 59 - 67.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
W. W. J. van de Sande, A. Fahal, H. Verbrugh, and A. van Belkum
Polymorphisms in Genes Involved in Innate Immunity Predispose Toward Mycetoma Susceptibility
J. Immunol., September 1, 2007; 179(5): 3065 - 3074.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Frantz, K. A. Vincent, O. Feron, and R. A. Kelly
Innate Immunity and Angiogenesis
Circ. Res., January 7, 2005; 96(1): 15 - 26.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Society for Investigative Pathology.