help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hart, S. P.
Right arrow Articles by Dransfield, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hart, S. P.
Right arrow Articles by Dransfield, I.
(American Journal of Pathology. 2003;162:1011-1018.)
© 2003 American Society for Investigative Pathology

Specific Binding of an Antigen-Antibody Complex to Apoptotic Human Neutrophils

Simon P. Hart*, Caroline Jackson*, L. Maximillian Kremmel*, Mary S. McNeill*, Hubertus Jersmann*, Karen M. Alexander*, James A. Ross{dagger} and Ian Dransfield*

From the Medical Research Council Centre for Inflammation Research,* University of Edinburgh Medical School, Edinburgh; and the Edinburgh University Department of Surgery,{dagger} Royal Infirmary, Edinburgh, Scotland

Examination of apoptotic cell surface molecules has so far failed to reveal cell type-specific membrane alterations that serve as a signal for phagocytosis. In the present study we have identified a novel murine monoclonal antibody, BOB93, which bound to the surface of apoptotic neutrophils but not to apoptotic lymphocytes. BOB93 binding to apoptotic neutrophils was dependent on the presence of the sialoglycoprotein fetuin, a constituent of bovine serum. We demonstrate that fetuin is the antigen for BOB93, and that BOB93 and fetuin form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. Individuals who were homozygous for an adenine nucleotide at position 519 of the gene for the immune complex receptor Fc{gamma}RIIA exhibited markedly reduced binding of BOB93/fetuin. This report is the first to provide evidence that antigen-antibody complexes bind specifically to apoptotic neutrophils and implicates apoptosis-associated changes in Fc{gamma} receptor function.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. J. Wigmore, K. C. H. Fearon, J. A. Ross, S. J. McNally, W. J. Welch, and O. J. Garden
Febrile-range temperature but not heat shock augments the acute phase response to interleukin-6 in human hepatoma cells
Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G903 - G911.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
S. Hart and I. Dransfield
Activation of human lung mast cells by monomeric immunoglobulin E
Eur. Respir. J., October 1, 2005; 26(4): 744 - 745.
[Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. L. Reynolds, J. N. Skepper, R. McNair, T. Kasama, K. Gupta, P. L. Weissberg, W. Jahnen-Dechent, and C. M. Shanahan
Multifunctional Roles for Serum Protein Fetuin-A in Inhibition of Human Vascular Smooth Muscle Cell Calcification
J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2920 - 2930.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. P. Hart, K. M. Alexander, and I. Dransfield
Immune Complexes Bind Preferentially to Fc{gamma}RIIA (CD32) on Apoptotic Neutrophils, Leading to Augmented Phagocytosis by Macrophages and Release of Proinflammatory Cytokines
J. Immunol., February 1, 2004; 172(3): 1882 - 1887.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society for Investigative Pathology.