| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Technical Advance |
From the Departments of Oto-Rhino-Laryngology, Head, and Neck Surgery,* Pathology, and the Institute of Virology, University of Cologne, Cologne, Germany; and the Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey
Recent analyses of head and neck squamous cell carcinomas revealed frequent infections by oncogenic human papillomavirus (HPV) type 16 in tonsillar carcinomas. Concerning involvement of risk factors, clinical course of the disease, and prognosis there are strong indications arguing that the HPV-positive tonsillar carcinomas may represent a separate tumor entity. Looking for a surrogate marker, which in further epidemiological studies could replace the laborious and expensive HPV detection and typing we analyzed p16 protein expression in 34 tonsillar carcinoma for correlation to HPV status and load of viral DNA. p16 has been shown to be of diagnostic value for clinical evaluation of cervical dysplasia. We found 53% of the tested tonsillar carcinomas to be HPV-positive. Fifty-six percent of all tumors tested were immunohistochemically positive for the p16 protein. In 16 of 18 of the HPV-positive carcinomas diffuse p16 expression was observed. In contrast, only one of the HPV-negative carcinomas showed focal p16 staining (P < 0.001). As determined by laser-assisted microdissection and quantitative real-time polymerase chain reaction, p16 expression correlated with the presence of HPV-DNA in the individual tumor specimens. Clinical outcome analysis revealed significant correlation of p16 expression with increased disease-free survival (P = 0.02). These data indicate that p16 is a technically simple immunohistological marker, applicable for routine pathological histology, and its prognostic value for survival is fully equivalent to HPV-DNA detection.
This article has been cited by other articles:
 |
|
 |
|
  B. Kumar, K. G. Cordell, J. S. Lee, F. P. Worden, M. E. Prince, H. H. Tran, G. T. Wolf, S. G. Urba, D. B. Chepeha, T. N. Teknos, et al. EGFR, p16, HPV Titer, Bcl-xL and p53, Sex, and Smoking As Indicators of Response to Therapy and Survival in Oropharyngeal Cancer J. Clin. Oncol., July 1, 2008; 26(19): 3128 - 3137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Fakhry, W. H. Westra, S. Li, A. Cmelak, J. A. Ridge, H. Pinto, A. Forastiere, and M. L. Gillison Improved Survival of Patients With Human Papillomavirus-Positive Head and Neck Squamous Cell Carcinoma in a Prospective Clinical Trial J Natl Cancer Inst, February 20, 2008; 100(4): 261 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Strati and P. F. Lambert Role of Rb-Dependent and Rb-Independent Functions of Papillomavirus E7 Oncogene in Head and Neck Cancer Cancer Res., December 15, 2007; 67(24): 11585 - 11593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Begum, M. L. Gillison, T. L. Nicol, and W. H. Westra Detection of Human Papillomavirus-16 in Fine-Needle Aspirates to Determine Tumor Origin in Patients with Metastatic Squamous Cell Carcinoma of the Head and Neck Clin. Cancer Res., February 15, 2007; 13(4): 1186 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Gillison Human Papillomavirus and Prognosis of Oropharyngeal Squamous Cell Carcinoma: Implications for Clinical Research in Head and Neck Cancers J. Clin. Oncol., December 20, 2006; 24(36): 5623 - 5625. [Full Text] [PDF]
|
|
|
|
|
|
C. Fakhry and M. L. Gillison Clinical Implications of Human Papillomavirus in Head and Neck Cancers J. Clin. Oncol., June 10, 2006; 24(17): 2606 - 2611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B Perez-Ordonez, M Beauchemin, and R C K Jordan Molecular biology of squamous cell carcinoma of the head and neck. J. Clin. Pathol., May 1, 2006; 59(5): 445 - 453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Weinberger, Z. Yu, B. G. Haffty, D. Kowalski, M. Harigopal, J. Brandsma, C. Sasaki, J. Joe, R. L. Camp, D. L. Rimm, et al. Molecular Classification Identifies a Subset of Human Papillomavirus-Associated Oropharyngeal Cancers With Favorable Prognosis J. Clin. Oncol., February 10, 2006; 24(5): 736 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Begum, D. Cao, M. Gillison, M. Zahurak, and W. H. Westra Tissue Distribution of Human Papillomavirus 16 DNA Integration in Patients with Tonsillar Carcinoma Clin. Cancer Res., August 15, 2005; 11(16): 5694 - 5699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Wang, B.-Y. Zheng, X.-D. Li, T. Angstrom, M. S. Lindstrom, and K.-L. Wallin Predictive Significance of the Alterations of p16INK4A, p14ARF, p53, and Proliferating Cell Nuclear Antigen Expression in the Progression of Cervical Cancer Clin. Cancer Res., April 1, 2004; 10(7): 2407 - 2414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Begum, M. L. Gillison, M. A. Ansari-Lari, K. Shah, and W. H. Westra Detection of Human Papillomavirus in Cervical Lymph Nodes: A Highly Effective Strategy for Localizing Site of Tumor Origin Clin. Cancer Res., December 15, 2003; 9(17): 6469 - 6475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Li, C. H. Thompson, D. Xin, Y. E. Cossart, C. J. O'Brien, E. B. McNeil, K. Gao, R. A. Scolyer, and B. R. Rose Absence of Human Papillomavirus in Tonsillar Squamous Cell Carcinomas from Chinese Patients Am. J. Pathol., December 1, 2003; 163(6): 2185 - 2189. [Abstract] [Full Text]
|
|
|
|
|
|
A. Handra-Luca, H. Bilal, J.-C. Bertrand, and P. Fouret Extra-Cellular Signal-Regulated ERK-1/ERK-2 Pathway Activation in Human Salivary Gland Mucoepidermoid Carcinoma: Association to Aggressive Tumor Behavior and Tumor Cell Proliferation Am. J. Pathol., September 1, 2003; 163(3): 957 - 967. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
