| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Division of Gastroenterology* and the Academic Unit of Cancer Studies, University Hospital, Queen’s Medical Centre, Nottingham, United Kingdom
Intestinal fibrosis and strictures frequently occur in Crohn’s disease but not ulcerative colitis. We have recently shown that, compared to myofibroblasts obtained from normal and ulcerative colitis tissue, myofibroblasts isolated from fibrotic Crohn’s disease mucosal samples express significantly lower amounts of transforming growth factor (TGF)-ß3, but the expression of TGF-ß2 was significantly greater. We now report that in myofibroblast cultures established from fibrotic Crohn’s disease mucosal samples there is significantly higher constitutive expression of tissue inhibitor of metalloproteinase (TIMP)-1 compared to similar cells isolated from normal or ulcerative colitis tissue. Myofibroblasts derived from normal mucosa and from mucosa affected by ulcerative colitis or Crohn’s disease also expressed matrix metalloproteinase (MMP)-1, MMP-2, and MMP-3 but did not express MMP-9. Recombinant (r) TGF-ß1 and rTGF-ß2, but not rTGF-ß3, induced expression of TIMP-1 in normal intestinal myofibroblasts. These studies illustrate a potential mechanism by which differential expression of isoforms of TGF-ß may lead to excessive deposition of extracellular matrix and stricture formation via TIMP-1-mediated inhibition of MMP activity.
This article has been cited by other articles:
 |
|
 |
|
  A Di Sabatino, C L Jackson, K M Pickard, M Buckley, L Rovedatti, N A B Leakey, L Picariello, P Cazzola, G Monteleone, F Tonelli, et al. Transforming growth factor {beta} signalling and matrix metalloproteinases in the mucosa overlying Crohn's disease strictures Gut, June 1, 2009; 58(6): 777 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Samuel, R. Walsh, J. Webb, A. Robins, C. Potten, and Y. R. Mahida Characterization of putative stem cells in isolated human colonic crypt epithelial cells and their interactions with myofibroblasts Am J Physiol Cell Physiol, February 1, 2009; 296(2): C296 - C305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Di Sabatino, K M Pickard, D Rampton, L Kruidenier, L Rovedatti, N A B Leakey, G R Corazza, G Monteleone, and T T MacDonald Blockade of transforming growth factor {beta} upregulates T-box transcription factor T-bet, and increases T helper cell type 1 cytokine and matrix metalloproteinase-3 production in the human gut mucosa Gut, May 1, 2008; 57(5): 605 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Bodger, S Ahmed, L Pazmany, D M Pritchard, A Micheal, A L Khan, R Dimaline, G J Dockray, and A Varro Altered gastric corpus expression of tissue inhibitors of metalloproteinases in human and murine Helicobacter infection J. Clin. Pathol., January 1, 2008; 61(1): 72 - 78. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sengupta and T. T. MacDonald The Role of Matrix Metalloproteinases in Stromal/Epithelial Interactions in the Gut Physiology, December 1, 2007; 22(6): 401 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heidemann, C. Ruther, M. Kebschull, W. Domschke, M. Bruwer, S. Koch, T. Kucharzik, and C. Maaser Expression of IL-12-related molecules in human intestinal microvascular endothelial cells is regulated by TLR3 Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1315 - G1324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Denning, S. Granger, D. Mucida, R. Graddy, G. Leclercq, W. Zhang, K. Honey, J. P. Rasmussen, H. Cheroutre, A. Y. Rudensky, et al. Mouse TCR{alpha}beta+CD8{alpha}{alpha} Intraepithelial Lymphocytes Express Genes That Down-Regulate Their Antigen Reactivity and Suppress Immune Responses J. Immunol., April 1, 2007; 178(7): 4230 - 4239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Peiris, I. Pacheco, C. Spencer, and R. J. MacLeod The extracellular calcium-sensing receptor reciprocally regulates the secretion of BMP-2 and the BMP antagonist Noggin in colonic myofibroblasts Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G753 - G766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Brun, I. Castagliuolo, V. D. Leo, A. Buda, M. Pinzani, G. Palu, and D. Martines Increased intestinal permeability in obese mice: new evidence in the pathogenesis of nonalcoholic steatohepatitis Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G518 - G525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Rieder, J Brenmoehl, S Leeb, J Scholmerich, and G Rogler Wound healing and fibrosis in intestinal disease Gut, January 1, 2007; 56(1): 130 - 139. [Full Text] [PDF]
|
|
|
|
|
|
P. Garg, M. Rojas, A. Ravi, K. Bockbrader, S. Epstein, M. Vijay-Kumar, A. T. Gewirtz, D. Merlin, and S. V. Sitaraman Selective Ablation of Matrix Metalloproteinase-2 Exacerbates Experimental Colitis: Contrasting Role of Gelatinases in the Pathogenesis of Colitis J. Immunol., September 15, 2006; 177(6): 4103 - 4112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Medina and M. W. Radomski Role of Matrix Metalloproteinases in Intestinal Inflammation J. Pharmacol. Exp. Ther., September 1, 2006; 318(3): 933 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sodek, A. P. Batista Da Silva, and R. Zohar Osteopontin and Mucosal Protection Journal of Dental Research, May 1, 2006; 85(5): 404 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Theiss, J. G. Simmons, C. Jobin, and P. K. Lund Tumor Necrosis Factor (TNF) {alpha} Increases Collagen Accumulation and Proliferation in Intestinal Myofibroblasts via TNF Receptor 2 J. Biol. Chem., October 28, 2005; 280(43): 36099 - 36109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Gratton, V. H. Rao, D. T. Meehan, C. Askew, and D. Cosgrove Matrix Metalloproteinase Dysregulation in the Stria Vascularis of Mice with Alport Syndrome: Implications for Capillary Basement Membrane Pathology Am. J. Pathol., May 1, 2005; 166(5): 1465 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Bourgier, V Haydont, F Milliat, A Francois, V Holler, P Lasser, J Bourhis, D Mathe, and M-C Vozenin-Brotons Inhibition of Rho kinase modulates radiation induced fibrogenic phenotype in intestinal smooth muscle cells through alteration of the cytoskeleton and connective tissue growth factor expression Gut, March 1, 2005; 54(3): 336 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhou, J. B. Trudeau, K. J. Schoonover, J. I. Lundin, S. M. Barnes, M. J. Cundall, and S. E. Wenzel Interleukin-13 augments transforming growth factor-{beta}1-induced tissue inhibitor of metalloproteinase-1 expression in primary human airway fibroblasts Am J Physiol Cell Physiol, February 1, 2005; 288(2): C435 - C442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Strup-Perrot, D. Mathe, C. Linard, D. Violot, F. Milliat, A. Francois, J. Bourhis, and M.-C. Vozenin-Brotons Global gene expression profiles reveal an increase in mRNA levels of collagens, MMPs, and TIMPs in late radiation enteritis Am J Physiol Gastrointest Liver Physiol, October 1, 2004; 287(4): G875 - G885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-A. Choi, H.-K. Lim, J.-R. Kim, C.-H. Lee, Y.-J. Kim, S.-S. Kang, and S.-H. Baek Group IB Secretory Phospholipase A2 Promotes Matrix Metalloproteinase-2-mediated Cell Migration via the Phosphatidylinositol 3-Kinase and Akt Pathway J. Biol. Chem., August 27, 2004; 279(35): 36579 - 36585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yamagami, S. Yokoo, T. Mimura, and S. Amano Effects of TGF-{beta}2 on Immune Response-Related Gene Expression Profiles in the Human Corneal Endothelium Invest. Ophthalmol. Vis. Sci., February 1, 2004; 45(2): 515 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Chitapanarux, S. L. Chen, H. Lee, A. C. Melton, and H. F. Yee Jr. C-type natriuretic peptide induces human colonic myofibroblast relaxation Am J Physiol Gastrointest Liver Physiol, January 1, 2004; 286(1): G31 - G36. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
