| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Departments of Medical Oncology* and Colorectal Surgery, Saint Vincent’s Hospital, Darlinghurst, Australia; and the Schools of Medicine and Medical Sciences, University of New South Wales, Sydney, Australia
Tumors are often characterized by an imbalance in cytosine methylation as manifested both by hypermethylation of CpG islands and by genome hypomethylation. These epigenetic changes were assessed in colorectal neoplasia to determine whether they arose through a common mechanism or indeed were distinct and unrelated phenomena. Fresh representative samples of adenomas, hyperplastic polyps, colorectal cancers, and normal mucosa were used in this study. Global methylation levels were measured by analyzing the methyl-accepting capacity of DNA. Methylation of p16, hMLH1, and MINT 1, 2, 12, and 31 were assessed by bisulfite polymerase chain reaction. Microsatellite status was determined by polymerase chain reaction using six markers and hMLH1 and proliferating cell nuclear antigen expression was assessed by immunohistochemistry. Normal colonic mucosa had a higher endogenous 5-methyl cytosine content than all proliferative lesions of the colon (P < 0.001). The extent of demethylation in hyperplastic polyps and adenomas was significantly related to its proliferative rate. Right-sided hyperplastic polyps were more likely to be methylated than adenomas (odds ratio, 2.3; confidence interval, 1.1 to 4.6). There was no relationship between the level of global hypomethylation and hypermethylation. Some hyperplastic colorectal polyps have a propensity to develop dense CpG island methylation. Hypermethylation and hypomethylation contribute separately to the process of carcinogenesis.
This article has been cited by other articles:
 |
|
 |
|
  S. de Vogel, K. A.D. Wouters, R. W.H. Gottschalk, F. J. van Schooten, A. F.P.M. de Goeij, A. P. de Bruine, R. A. Goldbohm, P. A. van den Brandt, M. P. Weijenberg, and M. van Engeland Genetic Variants of Methyl Metabolizing Enzymes and Epigenetic Regulators: Associations with Promoter CpG Island Hypermethylation in Colorectal Cancer Cancer Epidemiol. Biomarkers Prev., November 1, 2009; 18(11): 3086 - 3096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Figueiredo, M. V. Grau, K. Wallace, A. J. Levine, L. Shen, R. Hamdan, X. Chen, R. S. Bresalier, G. McKeown-Eyssen, R. W. Haile, et al. Global DNA Hypomethylation (LINE-1) in the Normal Colon and Lifestyle Characteristics and Dietary and Genetic Factors Cancer Epidemiol. Biomarkers Prev., April 1, 2009; 18(4): 1041 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Lightfoot, J. H. Barrett, T. Bishop, E. L. Northwood, G. Smith, M. J.V. Wilkie, R. J.C. Steele, F. A. Carey, T. J. Key, R. Wolf, et al. Methylene Tetrahydrofolate Reductase Genotype Modifies the Chemopreventive Effect of Folate in Colorectal Adenoma, but not Colorectal Cancer Cancer Epidemiol. Biomarkers Prev., September 1, 2008; 17(9): 2421 - 2430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. de Vogel, B. W.C. Bongaerts, K. A.D. Wouters, A. D.M. Kester, L. J. Schouten, A. F.P.M. de Goeij, A. P. de Bruine, R. A. Goldbohm, P. A. van den Brandt, M. van Engeland, et al. Associations of dietary methyl donor intake with MLH1 promoter hypermethylation and related molecular phenotypes in sporadic colorectal cancer Carcinogenesis, September 1, 2008; 29(9): 1765 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grunau, M.-E. Brun, I. Rivals, J. Selves, W. Hindermann, M. Favre-Mercuret, G. Granier, and A. De Sario BAGE Hypomethylation, A New Epigenetic Biomarker for Colon Cancer Detection Cancer Epidemiol. Biomarkers Prev., June 1, 2008; 17(6): 1374 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Jung, E. M. Poole, J. Bigler, J. Whitton, J. D. Potter, and C. M. Ulrich DNA Methyltransferase and Alcohol Dehydrogenase: Gene-Nutrient Interactions in Relation to Risk of Colorectal Polyps Cancer Epidemiol. Biomarkers Prev., February 1, 2008; 17(2): 330 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. van den Donk, M. van Engeland, L. Pellis, B. J.M. Witteman, F. J. Kok, J. Keijer, and E. Kampman Dietary Folate Intake in Combination with MTHFR C677T Genotype and Promoter Methylation of Tumor Suppressor and DNA Repair Genes in Sporadic Colorectal Adenomas Cancer Epidemiol. Biomarkers Prev., February 1, 2007; 16(2): 327 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J J L Wong, N J Hawkins, and R L Ward Colorectal cancer: a model for epigenetic tumorigenesis Gut, January 1, 2007; 56(1): 140 - 148. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Cadieux, T.-T. Ching, S. R. VandenBerg, and J. F. Costello Genome-wide Hypomethylation in Human Glioblastomas Associated with Specific Copy Number Alteration, Methylenetetrahydrofolate Reductase Allele Status, and Increased Proliferation. Cancer Res., September 1, 2006; 66(17): 8469 - 8476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Matsuzaki, G. Deng, H. Tanaka, S. Kakar, S. Miura, and Y. S. Kim The Relationship between Global Methylation Level, Loss of Heterozygosity, and Microsatellite Instability in Sporadic Colorectal Cancer Clin. Cancer Res., December 15, 2005; 11(24): 8564 - 8569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Ulrich Nutrigenetics in Cancer Research--Folate Metabolism and Colorectal Cancer J. Nutr., November 1, 2005; 135(11): 2698 - 2702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nishiyama, L. Qi, M. Lacey, and M. Ehrlich Both Hypomethylation and Hypermethylation in a 0.2-kb Region of a DNA Repeat in Cancer Mol. Cancer Res., November 1, 2005; 3(11): 617 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Woodson, D. J. Weisenberger, M. Campan, P. W. Laird, J. Tangrea, L. L. Johnson, A. Schatzkin, and E. Lanza Gene-Specific Methylation and Subsequent Risk of Colorectal Adenomas among Participants of the Polyp Prevention Trial Cancer Epidemiol. Biomarkers Prev., May 1, 2005; 14(5): 1219 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Pufulete, R Al-Ghnaniem, A Khushal, P Appleby, N Harris, S Gout, P W Emery, and T A B Sanders Effect of folic acid supplementation on genomic DNA methylation in patients with colorectal adenoma Gut, May 1, 2005; 54(5): 648 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Petko, M. Ghiassi, A. Shuber, J. Gorham, W. Smalley, M. K. Washington, S. Schultenover, S. Gautam, S. D. Markowitz, and W. M. Grady Aberrantly Methylated CDKN2A, MGMT, and MLH1 in Colon Polyps and in Fecal DNA from Patients with Colorectal Polyps Clin. Cancer Res., February 1, 2005; 11(3): 1203 - 1209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-C. Li, P. R. Carroll, and R. Dahiya Epigenetic Changes in Prostate Cancer: Implication for Diagnosis and Treatment J Natl Cancer Inst, January 19, 2005; 97(2): 103 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Frigola, X. Sole, M. F. Paz, V. Moreno, M. Esteller, G. Capella, and M. A. Peinado Differential DNA hypermethylation and hypomethylation signatures in colorectal cancer Hum. Mol. Genet., January 15, 2005; 14(2): 319 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Zauber, M Sabbath-Solitare, S Marotta, A Zauber, and T Bishop Comparative molecular pathology of sporadic hyperplastic polyps and neoplastic lesions from the same individual J. Clin. Pathol., October 1, 2004; 57(10): 1084 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Baker, Y Zhang, C Jin, and J R Jass Proximal versus distal hyperplastic polyps of the colorectum: different lesions or a biological spectrum? J. Clin. Pathol., October 1, 2004; 57(10): 1089 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Corwin The Concept of Epigenetics and Its Role in the Development of Cardiovascular Disease: Commentary on "New and Emerging Theories of Cardiovascular Disease" Biol Res Nurs, July 1, 2004; 6(1): 11 - 16. [PDF]
|
|
|
|
|
|
N. Yanagawa, G. Tamura, T. Honda, M. Endoh, S. Nishizuka, and T. Motoyama Demethylation of the Synuclein {gamma} Gene CpG Island in Primary Gastric Cancers and Gastric Cancer Cell Lines Clin. Cancer Res., April 1, 2004; 10(7): 2447 - 2451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawakami, A. Ruszkiewicz, G. Bennett, J. Moore, G. Watanabe, and B. Iacopetta The Folate Pool in Colorectal Cancers Is Associated with DNA Hypermethylation and with a Polymorphism in Methylenetetrahydrofolate Reductase Clin. Cancer Res., December 1, 2003; 9(16): 5860 - 5865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. van Engeland, M. P. Weijenberg, G. M. J. M. Roemen, M. Brink, A. P. de Bruine, R. A. Goldbohm, P. A. van den Brandt, S. B. Baylin, A. F. P. M. de Goeij, and J. G. Herman Effects of Dietary Folate and Alcohol Intake on Promoter Methylation in Sporadic Colorectal Cancer: The Netherlands Cohort Study on Diet and Cancer Cancer Res., June 15, 2003; 63(12): 3133 - 3137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Iacopetta Aberrant DNA Methylation: Have We Entered the Era of More Than One Type of Colorectal Cancer? Am. J. Pathol., April 1, 2003; 162(4): 1043 - 1045. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
