| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Departments of Pediatrics,* Cell Biology and Physiology, and Medicine, Washington University School of Medicine, St. Louis; and the Saint Louis Children’s Hospital, Saint Louis, Missouri
Matrilysin (matrix metalloproteinase-7) is highly expressed in lungs of patients with pulmonary fibrosis and other conditions associated with airway and alveolar injury. Although matrilysin is required for closure of epithelial wounds ex vivo, the mechanism of its action in repair is unknown. We demonstrate that matrilysin mediates shedding of E-cadherin ectodomain from injured lung epithelium both in vitro and in vivo. In alveolar-like epithelial cells, transfection of activated matrilysin resulted in shedding of E-cadherin and accelerated cell migration. In vivo, matrilysin co-localized with E-cadherin at the basolateral surfaces of migrating tracheal epithelium, and the reorganization of cell-cell junctions seen in wild-type injured tissue was absent in matrilysin-null samples. E-cadherin ectodomain was shed into the bronchoalveolar lavage fluid of bleomycin-injured wild-type mice, but was not shed in matrilysin-null mice. These findings identify E-cadherin as a novel substrate for matrilysin and indicate that shedding of E-cadherin ectodomain is required for epithelial repair.
This article has been cited by other articles:
 |
|
 |
|
  M. Swee, C. L. Wilson, Y. Wang, J. K. McGuire, and W. C. Parks Matrix metalloproteinase-7 (matrilysin) controls neutrophil egress by generating chemokine gradients J. Leukoc. Biol., June 1, 2008; 83(6): 1404 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chen, J. K. McGuire, R. C. Hackman, K.-H. Kim, R. A. Black, K. Poindexter, W. Yan, P. Liu, A. J. Chen, W. C. Parks, et al. Tissue Inhibitor of Metalloproteinase-1 Moderates Airway Re-Epithelialization by Regulating Matrilysin Activity Am. J. Pathol., May 1, 2008; 172(5): 1256 - 1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cao, C. Chiarelli, O. Richman, K. Zarrabi, P. Kozarekar, and S. Zucker Membrane Type 1 Matrix Metalloproteinase Induces Epithelial-to-Mesenchymal Transition in Prostate Cancer J. Biol. Chem., March 7, 2008; 283(10): 6232 - 6240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Fattman, F. Gambelli, G. Hoyle, B. R. Pitt, and L. A. Ortiz Epithelial expression of TIMP-1 does not alter sensitivity to bleomycin-induced lung injury in C57BL/6 mice Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L572 - L581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. T. Sawey, J. A. Johnson, and H. C. Crawford Matrix metalloproteinase 7 controls pancreatic acinar cell transdifferentiation by activating the Notch signaling pathway PNAS, December 4, 2007; 104(49): 19327 - 19332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Kassim, S. A. Gharib, B. H. Mecham, T. P. Birkland, W. C. Parks, and J. K. McGuire Individual Matrix Metalloproteinases Control Distinct Transcriptional Responses in Airway Epithelial Cells Infected with Pseudomonas aeruginosa Infect. Immun., December 1, 2007; 75(12): 5640 - 5650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Harris and J. D. Aplin Vascular Remodeling and Extracellular Matrix Breakdown in the Uterine Spiral Arteries During Pregnancy Reproductive Sciences, December 1, 2007; 14(8_suppl): 28 - 34. [Abstract] [PDF]
|
|
|
|
 |
|
 G. Chen, E. A. Bridenbaugh, A. D. Akintola, J. M. Catania, V. S. Vaidya, J. V. Bonventre, A. C. Dearman, H. W. Sampson, D. C. Zawieja, R. C. Burghardt, et al. Increased susceptibility of aging kidney to ischemic injury: identification of candidate genes changed during aging, but corrected by caloric restriction Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1272 - F1281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Navaratna, P. G. McGuire, G. Menicucci, and A. Das Proteolytic Degradation of VE-Cadherin Alters the Blood-Retinal Barrier in Diabetes Diabetes, September 1, 2007; 56(9): 2380 - 2387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wu, K.-J. Rhee, M. Zhang, A. Franco, and C. L. Sears Bacteroides fragilis toxin stimulates intestinal epithelial cell shedding and {gamma}-secretase-dependent E-cadherin cleavage J. Cell Sci., June 1, 2007; 120(11): 1944 - 1952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Reynolds, H. Shen, P. R. Reynolds, T. Betsuyaku, J. M. Pilewski, F. Gambelli, M. DeGuiseppe, L. A. Ortiz, and B. R. Stripp Molecular and functional properties of lung SP cells Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L972 - L983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. Hojilla, I. Kim, Z. Kassiri, J. E. Fata, H. Fang, and R. Khokha Metalloproteinase axes increase beta-catenin signaling in primary mouse mammary epithelial cells lacking TIMP3 J. Cell Sci., March 15, 2007; 120(6): 1050 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Greenlee, Z. Werb, and F. Kheradmand Matrix Metalloproteinases in Lung: Multiple, Multifarious, and Multifaceted Physiol Rev, January 1, 2007; 87(1): 69 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Puchelle, J.-M. Zahm, J.-M. Tournier, and C. Coraux Airway Epithelial Repair, Regeneration, and Remodeling after Injury in Chronic Obstructive Pulmonary Disease Proceedings of the ATS, November 1, 2006; 3(8): 726 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Illman, K. Lehti, J. Keski-Oja, and J. Lohi Epilysin (MMP-28) induces TGF-{beta} mediated epithelial to mesenchymal transition in lung carcinoma cells J. Cell Sci., September 15, 2006; 119(18): 3856 - 3865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Kelly, R. Leigh, S. E. Gilpin, E. Cheng, G. E. M. Martin, K. Radford, G. Cox, and J. Gauldie Cell-specific Gene Expression in Patients with Usual Interstitial Pneumonia Am. J. Respir. Crit. Care Med., September 1, 2006; 174(5): 557 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zhang, G. M. Dailey, E. Kwan, B. M. Glasheen, G. E. Sroga, and A. Page-McCaw An MMP liberates the Ninjurin A ectodomain to signal a lossof cell adhesion Genes & Dev., July 15, 2006; 20(14): 1899 - 1910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Koli, M. Myllarniemi, K. Vuorinen, K. Salmenkivi, M. J. Ryynanen, V. L. Kinnula, and J. Keski-Oja Bone Morphogenetic Protein-4 Inhibitor Gremlin Is Overexpressed in Idiopathic Pulmonary Fibrosis Am. J. Pathol., July 1, 2006; 169(1): 61 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chen, A. S. Farivar, M. S. Mulligan, and D. K. Madtes Tissue Inhibitor of Metalloproteinase-1 Deficiency Abrogates Obliterative Airway Disease after Heterotopic Tracheal Transplantation Am. J. Respir. Cell Mol. Biol., April 1, 2006; 34(4): 464 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Covington, R. C. Burghardt, and A. R. Parrish Ischemia-induced cleavage of cadherins in NRK cells requires MT1-MMP (MMP-14) Am J Physiol Renal Physiol, January 1, 2006; 290(1): F43 - F51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Davis and D. R. Senger Endothelial Extracellular Matrix: Biosynthesis, Remodeling, and Functions During Vascular Morphogenesis and Neovessel Stabilization Circ. Res., November 25, 2005; 97(11): 1093 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Ward, I A Forrest, D M Murphy, G E Johnson, H Robertson, T E Cawston, A J Fisher, J H Dark, J L Lordan, J A Kirby, et al. Phenotype of airway epithelial cells suggests epithelial to mesenchymal cell transition in clinically stable lung transplant recipients Thorax, October 1, 2005; 60(10): 865 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-H. Kim, K. Burkhart, P. Chen, C. W. Frevert, J. Randolph-Habecker, R. C. Hackman, P. D. Soloway, and D. K. Madtes Tissue Inhibitor of Metalloproteinase-1 Deficiency Amplifies Acute Lung Injury in Bleomycin-Exposed Mice Am. J. Respir. Cell Mol. Biol., September 1, 2005; 33(3): 271 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Jiang, G. Davies, T. A. Martin, C. Parr, G. Watkins, M. D. Mason, K. Mokbel, and R. E. Mansel Targeting Matrilysin and Its Impact on Tumor Growth In vivo: The Potential Implications in Breast Cancer Therapy Clin. Cancer Res., August 15, 2005; 11(16): 6012 - 6019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Covington, K. J. Bayless, R. C. Burghardt, G. E. Davis, and A. R. Parrish Ischemia-induced cleavage of cadherins in NRK cells: evidence for a role of metalloproteinases Am J Physiol Renal Physiol, August 1, 2005; 289(2): F280 - F288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Lazar, P. J. Christensen, M. Du, B. Yu, N. M. Subbotina, K. E. Hanson, J. M. Hansen, E. S. White, R. H. Simon, and T. H. Sisson Plasminogen Activator Inhibitor-1 Impairs Alveolar Epithelial Repair by Binding to Vitronectin Am. J. Respir. Cell Mol. Biol., December 1, 2004; 31(6): 672 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Park, T. J. Foster, E. Nishi, S. J. Duncan, M. Klagsbrun, and Y. Chen Activation of Syndecan-1 Ectodomain Shedding by Staphylococcus aureus {alpha}-Toxin and {beta}-Toxin J. Biol. Chem., January 2, 2004; 279(1): 251 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kaminski, J. A. Belperio, P. B. Bitterman, L. Chen, S. W. Chensue, A. M.K. Choi, S. Dacic, J. H. Dauber, R. M. du Bois, J. J. Enghild, et al. Idiopathic Pulmonary Fibrosis Am. J. Respir. Cell Mol. Biol., September 1, 2003; 29(3): S1 - 105. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
