help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamashiro, K.
Right arrow Articles by Adamis, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamashiro, K.
Right arrow Articles by Adamis, A. P.
(American Journal of Pathology. 2003;163:253-259.)
© 2003 American Society for Investigative Pathology

Platelets Accumulate in the Diabetic Retinal Vasculature Following Endothelial Death and Suppress Blood-Retinal Barrier Breakdown

Kenji Yamashiro*{dagger}, Akitaka Tsujikawa{dagger}, Susumu Ishida*{ddagger}, Tomohiko Usui*§, Yuichi Kaji*§, Yoshihito Honda{dagger}, Yuichiro Ogura and Anthony P. Adamis||

From the Angiogenesis/Retina Research Laboratory,* Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, Massachusetts; the Department of Ophthalmology and Visual Sciences,{dagger} Kyoto University Graduate School of Medicine, Kyoto, Japan; the Department of Ophthalmology,{ddagger} Keio University School of Medicine, Tokyo, Japan; the Department of Ophthalmology,§ Faculty of Medicine, University of Tokyo, Tokyo, Japan; the Department of Ophthalmology, Nagoya City University Medical School, Nagoya, Japan; and Eyetech Research Center,|| Woburn, Massachusetts

Platelet microthrombi are present in the diabetic retinal vasculature of humans and rodents; however, the mechanisms and consequences of their presence have not been defined. The current study demonstrates that platelet containing microthrombi accumulate in the retinal vasculature of the rat within 2 weeks of experimental diabetes, a timepoint at which leukocyte-mediated endothelial cell injury and death are known to occur. Platelet accumulation increased with the duration of diabetes, and crossover experiments revealed that maximal platelet accumulation required both diabetic platelets and a diabetic endothelium. Platelet accumulation also coincided with the expression of Fas and FasL in the diabetic retina. When endothelial cell apoptosis was inhibited with an anti-FasL neutralizing antibody, platelet accumulation was effectively suppressed. When platelets were depleted from the systemic circulation with an anti-platelet antibody, blood-retinal barrier breakdown worsened in the diabetic animals. These findings suggest that platelet accumulation in the diabetic retinal vasculature is secondary to endothelial cell death and serves, in part, to suppress blood-retinal barrier breakdown.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Kisucka, C. E. Butterfield, D. G. Duda, S. C. Eichenberger, S. Saffaripour, J. Ware, Z. M. Ruggeri, R. K. Jain, J. Folkman, and D. D. Wagner
Platelets and platelet adhesion support angiogenesis while preventing excessive hemorrhage
PNAS, January 24, 2006; 103(4): 855 - 860.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
K. Musashi, J. Kiryu, K. Miyamoto, S. Miyahara, H. Katsuta, H. Tamura, F. Hirose, and N. Yoshimura
Thrombin Inhibitor Reduces Leukocyte-Endothelial Cell Interactions and Vascular Leakage after Scatter Laser Photocoagulation
Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2561 - 2566.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
H. Tamura, K. Miyamoto, J. Kiryu, S. Miyahara, H. Katsuta, F. Hirose, K. Musashi, and N. Yoshimura
Intravitreal Injection of Corticosteroid Attenuates Leukostasis and Vascular Leakage in Experimental Diabetic Retina
Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1440 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Zheng, C. Szabo, and T. S. Kern
Poly(ADP-Ribose) Polymerase Is Involved in the Development of Diabetic Retinopathy via Regulation of Nuclear Factor-{kappa}B
Diabetes, November 1, 2004; 53(11): 2960 - 2967.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. M. A. El-Asrar, L. Dralands, L. Missotten, I. A. Al-Jadaan, and K. Geboes
Expression of Apoptosis Markers in the Retinas of Human Subjects with Diabetes
Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2760 - 2766.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
N. Cabeza, Z. Li, C. Schulz, E. Kremmer, S. Massberg, A. Bultmann, and M. Gawaz
Surface Expression of Collagen Receptor Fc Receptor-{gamma}/Glycoprotein VI Is Enhanced on Platelets in Type 2 Diabetes and Mediates Release of CD40 Ligand and Activation of Endothelial Cells
Diabetes, August 1, 2004; 53(8): 2117 - 2121.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. Brill, H. Elinav, and D. Varon
Differential role of platelet granular mediators in angiogenesis
Cardiovasc Res, August 1, 2004; 63(2): 226 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Miyahara, J. Kiryu, K. Yamashiro, K. Miyamoto, F. Hirose, H. Tamura, H. Katsuta, K. Nishijima, A. Tsujikawa, and Y. Honda
Simvastatin Inhibits Leukocyte Accumulation and Vascular Permeability in the Retinas of Rats with Streptozotocin-Induced Diabetes
Am. J. Pathol., May 1, 2004; 164(5): 1697 - 1706.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society for Investigative Pathology.