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(American Journal of Pathology. 2003;163:69-79.)
© 2003 American Society for Investigative Pathology

Molecular Characterization of NF-HEV, a Nuclear Factor Preferentially Expressed in Human High Endothelial Venules

Espen S. Baekkevold*, Myriam Roussigné{dagger}, Takeshi Yamanaka*, Finn-Eirik Johansen*, Frode L. Jahnsen*, François Amalric{dagger}, Per Brandtzaeg*, Monique Erard{ddagger}, Guttorm Haraldsen* and Jean-Philippe Girard{dagger}

From the Laboratory for Immunohistochemistry and Immunopathology (LIIPAT),* Institute and Department of Pathology, University of Oslo, Rikshospitalet, Oslo, Norway; the Laboratoire de Biologie Vasculaire,{dagger} and the Laboratoire des Interactions Acides Nucléiques/Protéines comme Cibles Pharmacologiques,{ddagger} Institut de Pharmacologie et de Biologie Structurale, CNRS-VMR 5089, Toulouse, France

Lymphocyte homing to secondary lymphoid tissue and lesions of chronic inflammation is directed by multi-step interactions between the circulating cells and the specialized endothelium of high endothelial venules (HEVs). In this study, we used the PCR-based method of suppression subtractive hybridization (SSH) to identify novel HEV genes by comparing freshly purified HEV endothelial cells (HEVECs) with nasal polyp-derived microvascular endothelial cells (PMECs). By this approach, we cloned the first nuclear factor preferentially expressed in HEVECs, designated nuclear factor from HEVs (NF-HEV). Virtual Northern and Western blot analyses showed strong expression of NF-HEV in HEVECs, compared to human umbilical vein endothelial cells (HUVECs) and PMECs. In situ hybridization and immunohistochemistry revealed that NF-HEV mRNA and protein are expressed at high levels and rather selectively by HEVECs in human tonsils, Peyers’s patches, and lymph nodes. The NF-HEV protein was found to contain a bipartite nuclear localization signal, and was targeted to the nucleus when ectopically expressed in HUVECs and HeLa cells. Furthermore, endogenous NF-HEV was found in situ to be confined to the nucleus of tonsillar HEVECs. Finally, threading and molecular modeling studies suggested that the amino-terminal part of NF-HEV (aa 1–60) corresponds to a novel homeodomain-like Helix-Turn-Helix (HTH) DNA-binding domain. Similarly to the atypical homeodomain transcription factor Prox-1, which plays a critical role in the induction of the lymphatic endothelium phenotype, NF-HEV may be one of the key nuclear factors that controls the specialized HEV phenotype.


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