| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From Institut National de la Santé et de la Recherche Mèdicale (INSERM) Unité Mixte de Recherche Santé (UMRS) 514,* Laboratoire Pol Bouin, Reims, France; the Laboratory of Tumor and Developmental Biology, University of Liège, Liège, Belgium; and the Department for Molecular Biomedical Research, Vlaams Interuni-versitair Institut Voor Biotechnologie-Ghent University, Ghent, Belgium
The disorganization of E-cadherin/catenin complexes and the overexpression of matrix metalloproteinases (MMPs) are frequently involved in the capacity of epithelial cells to acquire an invasive phenotype. The functional link between E-cadherin and MMPs was studied by transfecting invasive bronchial BZR tumor cells with human E-cadherin cDNA. Using different in vitro (cell dispersion, modified Boyden chamber) and in vivo assays (human airway epithelial xenograft), we showed that E-cadherin-positive clones displayed a decrease of invasive abilities. As shown by immunoprecipitation, the re-expressed E-cadherin was able to sequestrate one part of free cytoplasmic ß-catenin in BZR cells. The decrease of ß-catenin transcriptional activity in E-cadherin-transfected clones was demonstrated using the TOP-FLASH reporter construct. Finally, we observed a decrease of MMP-1, MMP-3, MMP-9, and MT1-MMP, both at the mRNA and at the protein levels, in E-cadherin-positive clones whereas no changes in MMP-2, TIMP-1, or TIMP-2 were observed when compared with control clones. Moreover, zymography analysis revealed a loss of MMP-2 activation ability in E-cadherin-positive clones treated with the concanavalin A lectin. These data demonstrate a direct role of E-cadherin/catenin complex organization in the regulation of MMPs and suggest an implication of this regulation in the expression of an invasive phenotype by bronchial tumor cells.
This article has been cited by other articles:
 |
|
 |
|
  U. Ullmann, C. Gilles, M. De Rycke, H. Van de Velde, K. Sermon, and I. Liebaers GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions Mol. Hum. Reprod., March 1, 2008; 14(3): 169 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Pollack, R. Sarkozi, Z. Banki, E. Feifel, S. Wehn, G. Gstraunthaler, H. Stoiber, G. Mayer, R. Montesano, F. Strutz, et al. Oncostatin M-induced effects on EMT in human proximal tubular cells: differential role of ERK signaling Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1714 - F1726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-W. Qian, J. Xie, F. Ye, and S.-J. Gao Kaposi's Sarcoma-Associated Herpesvirus Infection Promotes Invasion of Primary Human Umbilical Vein Endothelial Cells by Inducing Matrix Metalloproteinases J. Virol., July 1, 2007; 81(13): 7001 - 7010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-r. Zhao, W. Qiu, Y.-x. Li, Z.-b. Zhang, D. Li, and Y.-l. Wang Dual effect of transforming growth factor {beta}1 on cell adhesion and invasion in human placenta trophoblast cells. Reproduction, August 1, 2006; 132(2): 333 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C. Lunter, J. W.J. van Kilsdonk, H. van Beek, I. M.H.A. Cornelissen, M. Bergers, P. H.G.M. Willems, G. N.P. van Muijen, and G. W.M. Swart Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166/MEMD), a Novel Actor in Invasive Growth, Controls Matrix Metalloproteinase Activity Cancer Res., October 1, 2005; 65(19): 8801 - 8808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Jorda, D. Olmeda, A. Vinyals, E. Valero, E. Cubillo, A. Llorens, A. Cano, and A. Fabra Upregulation of MMP-9 in MDCK epithelial cell line in response to expression of the Snail transcription factor J. Cell Sci., August 1, 2005; 118(15): 3371 - 3385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lyu and C.-K. Joo Wnt-7a Up-regulates Matrix Metalloproteinase-12 Expression and Promotes Cell Proliferation in Corneal Epithelial Cells during Wound Healing J. Biol. Chem., June 3, 2005; 280(22): 21653 - 21660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Margulis, W. Zhang, A. Alt-Holland, H. C. Crawford, N. E. Fusenig, and J. A. Garlick E-cadherin Suppression Accelerates Squamous Cell Carcinoma Progression in Three-Dimensional, Human Tissue Constructs Cancer Res., March 1, 2005; 65(5): 1783 - 1791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lemmens, P. Fransen, S. U. Sys, D. L. Brutsaert, and G. W. De Keulenaer Neuregulin-1 Induces a Negative Inotropic Effect in Cardiac Muscle: Role of Nitric Oxide Synthase Circulation, January 27, 2004; 109(3): 324 - 326. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
