help button home button Am J Pathol Epitomics Buy 2 Antibodies Get 1 Free Special Offer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van den Boom, J.
Right arrow Articles by Hanash, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van den Boom, J.
Right arrow Articles by Hanash, S. M.
(American Journal of Pathology. 2003;163:1033-1043.)
© 2003 American Society for Investigative Pathology

Characterization of Gene Expression Profiles Associated with Glioma Progression Using Oligonucleotide-Based Microarray Analysis and Real-Time Reverse Transcription-Polymerase Chain Reaction

Jörg van den Boom*, Marietta Wolter*, Rork Kuick{dagger}, David E. Misek{dagger}, Andrew S. Youkilis{ddagger}, Daniel S. Wechsler{dagger}, Clemens Sommer§, Guido Reifenberger* and Samir M. Hanash{dagger}

From the Department of Neuropathology,*Heinrich-Heine-University, Düsseldorf, Germany; the Laboratory of Neuropathology,§University of Ulm, Ulm, Germany; and the Department of Pediatrics{dagger}and Neurosurgery,{ddagger}University of Michigan Medical Center, Ann Arbor, Michigan

Diffuse astrocytoma of World Health Organization (WHO) grade II has an inherent tendency to spontaneously progress to anaplastic astrocytoma (WHO grade III) and/or glioblastoma (WHO grade IV). The molecular basis of astrocytoma progression is still poorly understood, in particular with respect to the progression-associated changes at the mRNA level. Therefore, we compared the transcriptional profile of approximately 6800 genes in primary WHO grade II gliomas and corresponding recurrent high-grade (WHO grade III or IV) gliomas from eight patients using oligonucleotide-based microarray analysis. We identified 66 genes whose mRNA levels differed significantly (P < 0.01, >=2-fold change) between the primary and recurrent tumors. The microarray data were corroborated by real-time reverse transcription-polymerase chain reaction analysis of 12 selected genes, including 7 genes with increased expression and 5 genes with reduced expression on progression. In addition, the expression of these 12 genes was determined in an independent series of 43 astrocytic gliomas (9 diffuse astrocytomas, 10 anaplastic astrocytomas, 17 primary, and 7 secondary glioblastomas). These analyses confirmed that the transcript levels of nine of the selected genes (COL4A2, FOXM1, MGP, TOP2A, CENPF, IGFBP4, VEGFA, ADD3, and CAMK2G) differed significantly in WHO grade II astrocytomas as compared to anaplastic astrocytomas and/or glioblastomas. Thus, we identified and validated a set of interesting candidate genes whose differential expression likely plays a role in astrocytoma progression.




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
S. P. Reddy, R. Britto, K. Vinnakota, H. Aparna, H. K. Sreepathi, B. Thota, A. Kumari, B.M. Shilpa, M. Vrinda, S. Umesh, et al.
Novel Glioblastoma Markers with Diagnostic and Prognostic Value Identified through Transcriptome Analysis
Clin. Cancer Res., May 15, 2008; 14(10): 2978 - 2987.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
L. P. Petalidis, A. Oulas, M. Backlund, M. T. Wayland, L. Liu, K. Plant, L. Happerfield, T. C. Freeman, P. Poirazi, and V. P. Collins
Improved grading and survival prediction of human astrocytic brain tumors by artificial neural network analysis of gene expression microarray data
Mol. Cancer Ther., May 1, 2008; 7(5): 1013 - 1024.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Petrovic, R. H. Costa, L. F. Lau, P. Raychaudhuri, and A. L. Tyner
FoxM1 Regulates Growth Factor-induced Expression of Kinase-interacting Stathmin (KIS) to Promote Cell Cycle Progression
J. Biol. Chem., January 4, 2008; 283(1): 453 - 460.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. P. Piccaluga, C. Agostinelli, A. Califano, A. Carbone, L. Fantoni, S. Ferrari, A. Gazzola, A. Gloghini, S. Righi, M. Rossi, et al.
Gene Expression Analysis of Angioimmunoblastic Lymphoma Indicates Derivation from T Follicular Helper Cells and Vascular Endothelial Growth Factor Deregulation
Cancer Res., November 15, 2007; 67(22): 10703 - 10710.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
F. B. Furnari, T. Fenton, R. M. Bachoo, A. Mukasa, J. M. Stommel, A. Stegh, W. C. Hahn, K. L. Ligon, D. N. Louis, C. Brennan, et al.
Malignant astrocytic glioma: genetics, biology, and paths to treatment
Genes & Dev., November 1, 2007; 21(21): 2683 - 2710.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
D. Krex, B. Klink, C. Hartmann, A. von Deimling, T. Pietsch, M. Simon, M. Sabel, J. P. Steinbach, O. Heese, G. Reifenberger, et al.
Long-term survival with glioblastoma multiforme
Brain, October 1, 2007; 130(10): 2596 - 2606.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
J. BANYARD, L. M. HUTCHINSON, and B. R. ZETTER
Thymosin beta-NB Is the Human Isoform of Rat Thymosin beta15
Ann. N.Y. Acad. Sci., September 1, 2007; 1112(1): 286 - 296.
[Abstract] [Full Text] [PDF]

Home page
 Neuro OncolHome page
T.-T. Tran, M. Uhl, J. Y. Ma, L. Janssen, V. Sriram, S. Aulwurm, I. Kerr, A. Lam, H. K. Webb, A. M. Kapoun, et al.
Inhibiting TGF-{beta} signaling restores immune surveillance in the SMA-560 glioma model
Neuro-oncol, July 1, 2007; 9(3): 259 - 270.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
R. Martinez, F. Setien, C. Voelter, S. Casado, M. P. Quesada, G. Schackert, and M. Esteller
CpG island promoter hypermethylation of the pro-apoptotic gene caspase-8 is a common hallmark of relapsed glioblastoma multiforme
Carcinogenesis, June 1, 2007; 28(6): 1264 - 1268.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
I. Perez de Castro, G. de Carcer, and M. Malumbres
A census of mitotic cancer genes: new insights into tumor cell biology and cancer therapy
Carcinogenesis, May 1, 2007; 28(5): 899 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Pfister, C. Schlaeger, F. Mendrzyk, A. Wittmann, A. Benner, A. Kulozik, W. Scheurlen, B. Radlwimmer, and P. Lichter
Array-based profiling of reference-independent methylation status (aPRIMES) identifies frequent promoter methylation and consecutive downregulation of ZIC2 in pediatric medulloblastoma
Nucleic Acids Res., April 1, 2007; 35(7): e51 - e51.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Tan, P. Raychaudhuri, and R. H. Costa
Chk2 Mediates Stabilization of the FoxM1 Transcription Factor To Stimulate Expression of DNA Repair Genes
Mol. Cell. Biol., February 1, 2007; 27(3): 1007 - 1016.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Takahashi, C. Furukawa, A. Takano, N. Ishikawa, T. Kato, S. Hayama, C. Suzuki, W. Yasui, K. Inai, S. Sone, et al.
The Neuromedin U-Growth Hormone Secretagogue Receptor 1b/Neurotensin Receptor 1 Oncogenic Signaling Pathway as a Therapeutic Target for Lung Cancer
Cancer Res., October 1, 2006; 66(19): 9408 - 9419.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. K. Radhakrishnan, U. G. Bhat, D. E. Hughes, I-C. Wang, R. H. Costa, and A. L. Gartel
Identification of a Chemical Inhibitor of the Oncogenic Transcription Factor Forkhead Box M1
Cancer Res., October 1, 2006; 66(19): 9731 - 9735.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Liu, B. Dai, S.-H. Kang, K. Ban, F.-J. Huang, F. F. Lang, K. D. Aldape, T.-x. Xie, C. E. Pelloski, K. Xie, et al.
FoxM1B Is Overexpressed in Human Glioblastomas and Critically Regulates the Tumorigenicity of Glioma Cells.
Cancer Res., April 1, 2006; 66(7): 3593 - 3602.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
E. Colombo, S. G. Giannelli, R. Galli, E. Tagliafico, C. Foroni, E. Tenedini, S. Ferrari, S. Ferrari, G. Corte, A. Vescovi, et al.
Embryonic Stem-Derived Versus Somatic Neural Stem Cells: A Comparative Analysis of Their Developmental Potential and Molecular Phenotype
Stem Cells, April 1, 2006; 24(4): 825 - 834.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
I.-M. Kim, T. Ackerson, S. Ramakrishna, M. Tretiakova, I-C. Wang, T. V. Kalin, M. L. Major, G. A. Gusarova, H. M. Yoder, R. H. Costa, et al.
The Forkhead Box m1 Transcription Factor Stimulates the Proliferation of Tumor Cells during Development of Lung Cancer
Cancer Res., February 15, 2006; 66(4): 2153 - 2161.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. V. Kalin, I-C. Wang, T. J. Ackerson, M. L. Major, C. J. Detrisac, V. V. Kalinichenko, A. Lyubimov, and R. H. Costa
Increased Levels of the FoxM1 Transcription Factor Accelerate Development and Progression of Prostate Carcinomas in both TRAMP and LADY Transgenic Mice
Cancer Res., February 1, 2006; 66(3): 1712 - 1720.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. A. Schwartz, R. J. Weil, R. C. Thompson, Y. Shyr, J. H. Moore, S. A. Toms, M. D. Johnson, and R. M. Caprioli
Proteomic-Based Prognosis of Brain Tumor Patients Using Direct-Tissue Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
Cancer Res., September 1, 2005; 65(17): 7674 - 7681.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. W. Vogel, Z. Zhuang, J. Li, H. Okamoto, M. Furuta, Y.-S. Lee, W. Zeng, E. H. Oldfield, A. O. Vortmeyer, and R. J. Weil
Proteins and Protein Pattern Differences between Glioma Cell Lines and Glioblastoma Multiforme
Clin. Cancer Res., May 15, 2005; 11(10): 3624 - 3632.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Liang, M. Diehn, N. Watson, A. W. Bollen, K. D. Aldape, M. K. Nicholas, K. R. Lamborn, M. S. Berger, D. Botstein, P. O. Brown, et al.
Gene expression profiling reveals molecularly and clinically distinct subtypes of glioblastoma multiforme
PNAS, April 19, 2005; 102(16): 5814 - 5819.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. M. Nigro, A. Misra, L. Zhang, I. Smirnov, H. Colman, C. Griffin, N. Ozburn, M. Chen, E. Pan, D. Koul, et al.
Integrated Array-Comparative Genomic Hybridization and Expression Array Profiles Identify Clinically Relevant Molecular Subtypes of Glioblastoma
Cancer Res., March 1, 2005; 65(5): 1678 - 1686.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Bostrom, A. F. Zebboudj, Y. Yao, T. S. Lin, and A. Torres
Matrix GLA Protein Stimulates VEGF Expression through Increased Transforming Growth Factor-{beta}1 Activity in Endothelial Cells
J. Biol. Chem., December 17, 2004; 279(51): 52904 - 52913.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
W. A. Freije, F. E. Castro-Vargas, Z. Fang, S. Horvath, T. Cloughesy, L. M. Liau, P. S. Mischel, and S. F. Nelson
Gene Expression Profiling of Gliomas Strongly Predicts Survival
Cancer Res., September 15, 2004; 64(18): 6503 - 6510.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
F. Bianchi, J. Hu, G. Pelosi, R. Cirincione, M. Ferguson, C. Ratcliffe, P. P. Di Fiore, K. Gatter, F. Pezzella, and U. Pastorino
Lung Cancers Detected by Screening with Spiral Computed Tomography Have a Malignant Phenotype when Analyzed by cDNA Microarray
Clin. Cancer Res., September 15, 2004; 10(18): 6023 - 6028.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
V. V. Kalinichenko, M. L. Major, X. Wang, V. Petrovic, J. Kuechle, H. M. Yoder, M. B. Dennewitz, B. Shin, A. Datta, P. Raychaudhuri, et al.
Foxm1b transcription factor is essential for development of hepatocellular carcinomas and is negatively regulated by the p19ARF tumor suppressor
Genes & Dev., April 1, 2004; 18(7): 830 - 850.
[Abstract] [Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
C. B. Knobbe, J. Reifenberger, B. Blaschke, and G. Reifenberger
Hypermethylation and Transcriptional Downregulation of the Carboxyl-Terminal Modulator Protein Gene in Glioblastomas
J Natl Cancer Inst, March 17, 2004; 96(6): 483 - 486.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society for Investigative Pathology.