help button home button Am J Pathol ASIP MEMBERSHIP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kataoka, K.
Right arrow Articles by Huh, N.-h.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kataoka, K.
Right arrow Articles by Huh, N.-h.
(American Journal of Pathology. 2003;163:1227-1231.)
© 2003 American Society for Investigative Pathology

Short Communication

Participation of Adult Mouse Bone Marrow Cells in Reconstitution of Skin

Ken Kataoka*, Reinhold J. Medina*, Tomofumi Kageyama*, Masahiro Miyazaki*, Tadashi Yoshino{dagger}, Teruhiko Makino{ddagger} and Nam-ho Huh*

From the Departments of Cell Biology* and Pathology,{dagger} Okayama University Graduate School of Medicine and Dentistry, Shikatachou, Okayama; and the Department of Dermatology,{ddagger} Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan

Results of recent studies have indicated that bone marrow cells can differentiate into various cells of ectodermal, mesodermal, and endodermal origins when transplanted into the body. However, the problems associated with those experiments such as the long latent period, rareness of the event, and difficulty in controlling the processes have hampered detailed mechanistic studies. In the present study, we examined the potency of mouse bone marrow cells to differentiate into cells comprising skin tissues using a skin reconstitution assay. Bone marrow cells from adult green fluorescent protein (GFP)-transgenic mice were transplanted in a mixture of embryonic mouse skin cells (17.5 days post-coitus) onto skin defects made on the backs of nude mice. Within 3 weeks, fully differentiated skin with hair was reconstituted. GFP-positive cells were found in the epidermis, hair follicles, sebaceous glands, and dermis. The localization and morphology of the cells, results of immunohistochemistry, and results of specific staining confirmed that the bone marrow cells had differentiated into epidermal keratinocytes, sebaceous gland cells, follicular epithelial cells, dendritic cells, and endothelial cells under the present conditions. These results indicate that this system is suitable for molecular and cellular mechanistic studies on differentiation of stem cells to various epidermal and dermal cells.




This article has been cited by other articles:


Home page
 Stem CellsHome page
M. Kucia, R. Reca, K. Miekus, J. Wanzeck, W. Wojakowski, A. Janowska-Wieczorek, J. Ratajczak, and M. Z. Ratajczak
Trafficking of Normal Stem Cells and Metastasis of Cancer Stem Cells Involve Similar Mechanisms: Pivotal Role of the SDF-1-CXCR4 Axis
Stem Cells, August 1, 2005; 23(7): 879 - 894.
[Abstract] [Full Text] [PDF]

Home page
 JRSMHome page
H T Hassan and M El-Sheemy
Adult bone-marrow stem cells and their potential in medicine
J R Soc Med, October 1, 2004; 97(10): 465 - 471.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society for Investigative Pathology.