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(American Journal of Pathology. 2003;163:1743-1750.)
© 2003 American Society for Investigative Pathology

Technical Advance

Function and Structure of Pressurized and Perfused Porcine Carotid Arteries

Effects of in Vitro Balloon Angioplasty

Jop Perrée*, Ton G. van Leeuwen*, Raphaella Kerindongo*, Jos A. E. Spaan{dagger} and Ed VanBavel{dagger}

From the Laser Center* and the Department of Medical Physics,{dagger} Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

In this report we describe the application of an in vitro pressure-perfusion system for study of functional/structural changes after in vitro balloon dilation injury. Pig carotid arteries were perfused at P = 100 mm Hg and Q = 100 ml/min, balloon angioplastied (BA), and cultured under these hemodynamic conditions for 4 or 8 days (n = 5 BA and 6 controls for each time point). To assess endothelial function, outer diameter changes in response to bradykinin (BK) were measured daily. Remodeling was determined from the shift in pressure-passive diameter relation, as obtained after papaverine addition. Arterial samples were processed for histology. Control arteries showed spontaneous tone, BK-induced relaxation, and inward remodeling that was more pronounced at day 8 (ratio end-to-start passive diameter at P = 100 mm Hg, 0.69 ± 0.04; P < 0.001) than at day 4 (0.85 ± 0.03, P = 0.03). Intimal hyperplasia was detectable in these control vessels at day 8 with accumulation of {alpha}-smooth muscle actin-positive cells around the lumen. Angioplasty caused ruptures and dissections and abolished tone that returned after 5 days of perfusion along with BK-dependent relaxation. No significant inward remodeling or intimal hyperplasia was observed at day 8 after angioplasty. Thus, BA inhibits remodeling, which occurs after in vitro perfusion of conductance arteries.






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Copyright © 2003 by the American Society for Investigative Pathology.