This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wong, K. T. Articles by Deubel, V. Search for Related Content PubMed PubMed Citation Articles by Wong, K. T. Articles by Deubel, V.

(American Journal of Pathology. 2003;163:2127-2137.)
© 2003 American Society for Investigative Pathology

Animal Model

A Golden Hamster Model for Human Acute Nipah Virus Infection

K. Thong Wong^*, Isabelle Grosjean, Christine Brisson, Barissa Blanquier, Michelle Fevre-Montange, Arlette Bernard, Philippe Loth, Marie-Claude Georges-Courbot, Michelle Chevallier^¶, Hideo Akaoka, Philippe Marianneau, Sai Kit Lam^*, T. Fabian Wild and Vincent Deubel

From the Faculty of Medicine,^* University of Malaya, Kuala Lumpur, Malaysia; Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre de Recherche Mérieux-Pasteur, Lyon, France; INSERM U.433, Lyon, France; INSERM U.404, Centre d’Etudes et de Recherche en Virologie et Immunologie, Lyon, France; and the Laboratoire Marcel Mérieux,^¶ Lyon, France

A predominantly pig-to-human zoonotic infection caused by the novel Nipah virus emerged recently to cause severe morbidity and mortality in both animals and man. Human autopsy studies showed the pathogenesis to be related to systemic vasculitis that led to widespread thrombotic occlusion and microinfarction in most major organs especially in the central nervous system. There was also evidence of extravascular parenchymal infection, particularly near damaged vessels (Wong KT, Shieh WJ, Kumar S, Norain K, Abdullah W, Guarner J, Goldsmith CS, Chua KB, Lam SK, Tan CT, Goh KJ, Chong HT, Jusoh R, Rollin PE, Ksiazek TG, Zaki SR, Nipah Virus Pathology Working Group: Nipah virus infection: Pathology and pathogenesis of an emerging paramyxoviral zoonosis. Am J Pathol 2002, 161:2153–2167). We describe here a golden hamster (Mesocricetus auratus) model that appears to reproduce the pathology and pathogenesis of acute human Nipah infection. Hamsters infected by intranasal or intraperitoneal routes died within 9 to 29 days or 5 to 9 days, respectively. Pathological lesions were most severe and extensive in the hamster brain. Vasculitis, thrombosis, and more rarely, multinucleated endothelial syncytia, were found in blood vessels of multiple organs. Viral antigen and RNA were localized in both vascular and extravascular tissues including neurons, lung, kidney, and spleen, as demonstrated by immunohistochemistry and in situ hybridization, respectively. Paramyxoviral-type nucleocapsids were identified in neurons and in vessel walls. At the terminal stage of infection, virus and/or viral RNA could be recovered from most solid organs and urine, but not from serum. The golden hamster is proposed as a suitable model for further studies including pathogenesis studies, anti-viral drug testing, and vaccine development against acute Nipah infection.

This article has been cited by other articles:

				F. J. Torres-Velez, W.-J. Shieh, P. E. Rollin, T. Morken, C. Brown, T. G. Ksiazek, and S. R. Zaki Histopathologic and Immunohistochemical Characterization of Nipah Virus Infection in the Guinea Pig Veterinary Pathology, July 1, 2008; 45(4): 576 - 585. [Abstract] [Full Text] [PDF]

				K. C. Yaiw, J. Bingham, G. Crameri, B. Mungall, A. Hyatt, M. Yu, B. Eaton, D. Shamala, L.-F. Wang, and K. Thong Wong Tioman Virus, a Paramyxovirus of Bat Origin, Causes Mild Disease in Pigs and Has a Predilection for Lymphoid Tissues J. Virol., January 1, 2008; 82(1): 565 - 568. [Abstract] [Full Text] [PDF]

				B. A. Mungall, D. Middleton, G. Crameri, J. Bingham, K. Halpin, G. Russell, D. Green, J. McEachern, L. I. Pritchard, B. T. Eaton, et al. Feline Model of Acute Nipah Virus Infection and Protection with a Soluble Glycoprotein-Based Subunit Vaccine J. Virol., December 15, 2006; 80(24): 12293 - 12302. [Abstract] [Full Text] [PDF]

				K. Hagmaier, N. Stock, S. Goodbourn, L.-F. Wang, and R. Randall A single amino acid substitution in the V protein of Nipah virus alters its ability to block interferon signalling in cells from different species J. Gen. Virol., December 1, 2006; 87(12): 3649 - 3653. [Abstract] [Full Text] [PDF]

				H. M. Weingartl, Y. Berhane, J. L. Caswell, S. Loosmore, J.-C. Audonnet, J. A. Roth, and M. Czub Recombinant Nipah Virus Vaccines Protect Pigs against Challenge J. Virol., August 15, 2006; 80(16): 7929 - 7938. [Abstract] [Full Text] [PDF]

				M. C. Georges-Courbot, H. Contamin, C. Faure, P. Loth, S. Baize, P. Leyssen, J. Neyts, and V. Deubel Poly(I)-Poly(C12U) but Not Ribavirin Prevents Death in a Hamster Model of Nipah Virus Infection Antimicrob. Agents Chemother., May 1, 2006; 50(5): 1768 - 1772. [Abstract] [Full Text] [PDF]

				M. I. Bonaparte, A. S. Dimitrov, K. N. Bossart, G. Crameri, B. A. Mungall, K. A. Bishop, V. Choudhry, D. S. Dimitrov, L.-F. Wang, B. T. Eaton, et al. From The Cover: Ephrin-B2 ligand is a functional receptor for Hendra virus and Nipah virus PNAS, July 26, 2005; 102(30): 10652 - 10657. [Abstract] [Full Text] [PDF]

				B. B. Gowen, D. L. Barnard, D. F. Smee, M.-H. Wong, A. M. Pace, K.-H. Jung, S. G. Winslow, K. W. Bailey, L. M. Blatt, and R. W. Sidwell Interferon Alfacon-1 Protects Hamsters from Lethal Pichinde Virus Infection Antimicrob. Agents Chemother., June 1, 2005; 49(6): 2378 - 2386. [Abstract] [Full Text] [PDF]

				C. Vogt, M. Eickmann, S. Diederich, M. Moll, and A. Maisner Endocytosis of the Nipah Virus Glycoproteins J. Virol., March 15, 2005; 79(6): 3865 - 3872. [Abstract] [Full Text] [PDF]