| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Departments of Pathology,* Genetics, and Biochemistry,** and Howard Hughes Medical Institute,¶ Stanford University Medical Center, Stanford, California; the Department of Statistics,|| Stanford University, Stanford, California; the Department of Pathology and Genetic Pathology Evaluation Centre, Vancouver General Hospital, Vancouver, British Columbia, Canada; the Department of Anatomical Pathology, University of Washington Medical Center, Seattle, Washington; and the Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, Ohio
Dermatofibrosarcoma protuberans (DFSP) is an aggressive spindle cell neoplasm. It is associated with the chromosomal translocation, t(17:22), which fuses the COL1A1 and PDGFß genes. We determined the characteristic gene expression profile of DFSP and characterized DNA copy number changes in DFSP by array-based comparative genomic hybridization (array CGH). Fresh frozen and formalin-fixed, paraffin-embedded samples of DFSP were analyzed by array CGH (four cases) and DNA microarray analysis of global gene expression (nine cases). The nine DFSPs were readily distinguished from 27 other diverse soft tissue tumors based on their gene expression patterns. Genes characteristically expressed in the DFSPs included PDGFß and its receptor, PDGFRB, APOD, MEOX1, PLA2R, and PRKCA. Array CGH of DNA extracted either from frozen tumor samples or from paraffin blocks yielded equivalent results. Large areas of chromosomes 17q and 22q, bounded by COL1A1 and PDGFß, respectively, were amplified in DFSP. Expression of genes in the amplified regions was significantly elevated. Our data shows that: 1) DFSP has a distinctive gene expression profile; 2) array CGH can be applied successfully to frozen or formalin-fixed, paraffin-embedded tumor samples; 3) a characteristic amplification of sequences from chromosomes 17q and 22q, demarcated by the COL1A1 and PDGFß genes, respectively, was associated with elevated expression of the amplified genes.
This article has been cited by other articles:
 |
|
 |
|
  F. Jaggi, M. A. Cabrita, A.-K. T. Perl, and G. Christofori Modulation of Endocrine Pancreas Development but not {beta}-Cell Carcinogenesis by Sprouty4 Mol. Cancer Res., March 1, 2008; 6(3): 468 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Lee, I. Espinosa, S. Vrijaldenhoven, S. Subramanian, K. D. Montgomery, S. Zhu, R. J. Marinelli, J. L. Peterse, N. Poulin, T. O. Nielsen, et al. Prognostic Significance of Macrophage Infiltration in Leiomyosarcomas Clin. Cancer Res., March 1, 2008; 14(5): 1423 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Leong, K. Niessen, I. Kulic, A. Raouf, C. Eaves, I. Pollet, and A. Karsan Jagged1-mediated Notch activation induces epithelial-to-mesenchymal transition through Slug-induced repression of E-cadherin J. Exp. Med., November 26, 2007; 204(12): 2935 - 2948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Garg, D. Chaurasiya, R. Rana, N. Jagadish, D. Kanojia, N. Dudha, N. Kamran, S. Salhan, A. Bhatnagar, S. Suri, et al. Sperm-Associated Antigen 9, a Novel Cancer Testis Antigen, Is a Potential Target for Immunotherapy in Epithelial Ovarian Cancer Clin. Cancer Res., March 1, 2007; 13(5): 1421 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Szollosi, B. Scholtz, K. Egervari, and Z. Nemes Transformed dermatofibrosarcoma protuberans: real time polymerase chain reaction detection of COL1A1-PDGFB fusion transcripts in sarcomatous areas J. Clin. Pathol., February 1, 2007; 60(2): 190 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. West, B. P. Rubin, M. A. Miller, S. Subramanian, G. Kaygusuz, K. Montgomery, S. Zhu, R. J. Marinelli, A. De Luca, E. Downs-Kelly, et al. A landscape effect in tenosynovial giant-cell tumor from activation of CSF1 expression by a translocation in a minority of tumor cells PNAS, January 17, 2006; 103(3): 690 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Nambiar, S. R. Boutin, R. Raja, and D. W. Rosenberg Global Gene Expression Profiling: A Complement to Conventional Histopathologic Analysis of Neoplasia Vet. Pathol., November 1, 2005; 42(6): 735 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Baird, S. Davis, C. R. Antonescu, U. L. Harper, R. L. Walker, Y. Chen, A. A. Glatfelter, P. H. Duray, and P. S. Meltzer Gene Expression Profiling of Human Sarcomas: Insights into Sarcoma Biology Cancer Res., October 15, 2005; 65(20): 9226 - 9235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Levin, D. Ghosh, K. R. Cho, and S. L. R. Kardia A model-based scan statistic for identifying extreme chromosomal regions of gene expression in human tumors Bioinformatics, June 15, 2005; 21(12): 2867 - 2874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. P. Li, J. A. Fletcher, M. C. Heinrich, J. E. Garber, S. E. Sallan, C. Curiel-Lewandrowski, A. Duensing, M. van de Rijn, L. E. Schnipper, and G. D. Demetri Familial Gastrointestinal Stromal Tumor Syndrome: Phenotypic and Molecular Features in a Kindred J. Clin. Oncol., April 20, 2005; 23(12): 2735 - 2743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. McArthur, G. D. Demetri, A. van Oosterom, M. C. Heinrich, M. Debiec-Rychter, C. L. Corless, Z. Nikolova, S. Dimitrijevic, and J. A. Fletcher Molecular and Clinical Analysis of Locally Advanced Dermatofibrosarcoma Protuberans Treated With Imatinib: Imatinib Target Exploration Consortium Study B2225 J. Clin. Oncol., February 1, 2005; 23(4): 866 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. DeVries, S. Nyante, J. Korkola, R. Segraves, K. Nakao, D. Moore, H. Bae, M. Wilhelm, S. Hwang, and F. Waldman Array-Based Comparative Genomic Hybridization from Formalin-Fixed, Paraffin-Embedded Breast Tumors J. Mol. Diagn., February 1, 2005; 7(1): 65 - 71. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
