help button home button Am J Pathol Epitomics, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hong, K. U.
Right arrow Articles by Stripp, B. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hong, K. U.
Right arrow Articles by Stripp, B. R.
(American Journal of Pathology. 2004;164:577-588.)
© 2004 American Society for Investigative Pathology

Basal Cells Are a Multipotent Progenitor Capable of Renewing the Bronchial Epithelium

Kyung U. Hong*, Susan D. Reynolds*{dagger}, Simon Watkins{ddagger}, Elaine Fuchs§ and Barry R. Stripp*{dagger}{ddagger}

From the Department of Environmental Medicine,* University of Rochester, Rochester, New York; the Departments of Environmental and Occupational Health,{dagger} and Cell Biology and Physiology,{ddagger} University of Pittsburgh, Pittsburgh, Pennsylvania; and the Laboratory of Mammalian Cell Biology and Development,§ Rockefeller University, New York, New York

Commitment of the pulmonary epithelium to bronchial and bronchiolar airway lineages occurs during the transition from pseudoglandular to cannalicular phases of lung development, suggesting that regional differences exist with respect to the identity of stem and progenitor cells that contribute to epithelial maintenance in adulthood. We previously defined a critical role for Clara cell secretory protein-expressing (CE) cells in renewal of bronchiolar airway epithelium following injury. Even though CE cells are also the principal progenitor for maintenance of the bronchial airway epithelium, CE cell injury is resolved through a mechanism involving recruitment of a second progenitor cell population that we now identify as a GSI-B4 reactive, cytokeratin-14-expressing basal cell. These cells exhibit multipotent differentiation capacity as assessed by analysis of cellular phenotype within clones of LacZ-tagged cells. Clones were derived from K14-expressing cells tagged in a cell-type-specific fashion by ligand-regulable Cre recombinase-mediated genomic rearrangement of the ROSA26 recombination substrate allele. We conclude that basal cells represent an alternative multipotent progenitor cell population of bronchial airways and that progenitor cell selection is dictated by the type of airway injury.




This article has been cited by other articles:


Home page
 Eur Respir JHome page
S. de Seranno and R. Meuwissen
Progress and applications of mouse models for human lung cancer
Eur. Respir. J., February 1, 2010; 35(2): 426 - 443.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. M. Hoffman, A. Shifren, M. R. Mazan, A. M. Gruntman, K. M. Lascola, R. D. Nolen-Walston, C. F. Kim, L. Tsai, R. A. Pierce, R. P. Mecham, et al.
Matrix modulation of compensatory lung regrowth and progenitor cell proliferation in mice
Am J Physiol Lung Cell Mol Physiol, February 1, 2010; 298(2): L158 - L168.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Morimoto, Z. Liu, H.-T. Cheng, N. Winters, D. Bader, and R. Kopan
Canonical Notch signaling in the developing lung is required for determination of arterial smooth muscle cells and selection of Clara versus ciliated cell fate
J. Cell Sci., January 15, 2010; 123(2): 213 - 224.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
M. Konigshoff and O. Eickelberg
WNT Signaling in Lung Disease: A Failure or a Regeneration Signal?
Am. J. Respir. Cell Mol. Biol., January 1, 2010; 42(1): 21 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. C. Zemke, R. M. Teisanu, A. Giangreco, J. A. Drake, B. L. Brockway, S. D. Reynolds, and B. R. Stripp
{beta}-Catenin Is Not Necessary for Maintenance or Repair of the Bronchiolar Epithelium
Am. J. Respir. Cell Mol. Biol., November 1, 2009; 41(5): 535 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. Maouche, M. Polette, T. Jolly, K. Medjber, I. Cloez-Tayarani, J.-P. Changeux, H. Burlet, C. Terryn, C. Coraux, J.-M. Zahm, et al.
{alpha}7 Nicotinic Acetylcholine Receptor Regulates Airway Epithelium Differentiation by Controlling Basal Cell Proliferation
Am. J. Pathol., November 1, 2009; 175(5): 1868 - 1882.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J.-A. Park and D. J. Tschumperlin
Chronic Intermittent Mechanical Stress Increases MUC5AC Protein Expression
Am. J. Respir. Cell Mol. Biol., October 1, 2009; 41(4): 459 - 466.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Giangreco, E. N. Arwert, I. R. Rosewell, J. Snyder, F. M. Watt, and B. R. Stripp
From the Cover: Stem cells are dispensable for lung homeostasis but restore airways after injury
PNAS, June 9, 2009; 106(23): 9286 - 9291.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
K. B. Adler and S. Matalon
Highlights of the June Issue
Am. J. Respir. Cell Mol. Biol., June 1, 2009; 40(6): 631 - 632.
[Full Text] [PDF]

Home page
 CarcinogenesisHome page
E. Habib Dakir, L. Feigenbaum, and R. I. Linnoila
Constitutive expression of human keratin 14 gene in mouse lung induces premalignant lesions and squamous differentiation
Carcinogenesis, December 1, 2008; 29(12): 2377 - 2384.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
E.L. Rawlins, T. Okubo, J. Que, Y. Xue, C. Clark, X. Luo, and B.L.M. Hogan
Epithelial Stem/Progenitor Cells in Lung Postnatal Growth, Maintenance, and Repair
Cold Spring Harb Symp Quant Biol, November 26, 2008; (2008) sqb.2008.73.037v2.
[Abstract] [PDF]

Home page
 Proc Am Thorac SocHome page
W. V. Cardoso and J. A. Whitsett
Resident Cellular Components of the Lung: Developmental Aspects
Proceedings of the ATS, September 15, 2008; 5(7): 767 - 771.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
R. G. Crystal, S. H. Randell, J. F. Engelhardt, J. Voynow, and M. E. Sunday
Airway Epithelial Cells: Current Concepts and Challenges
Proceedings of the ATS, September 15, 2008; 5(7): 772 - 777.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
T. W. Gilbert, S. Gilbert, M. Madden, S. D. Reynolds, and S. F. Badylak
Morphologic Assessment of Extracellular Matrix Scaffolds for Patch Tracheoplasty in a Canine Model
Ann. Thorac. Surg., September 1, 2008; 86(3): 967 - 974.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. L. Rawlins
Lung Epithelial Progenitor Cells: Lessons from Development
Proceedings of the ATS, August 15, 2008; 5(6): 675 - 681.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
H. J. Rippon, S. Lane, M. Qin, N.-S. Ismail, M. R. Wilson, M. Takata, and A. E. Bishop
Embryonic Stem Cells as a Source of Pulmonary Epithelium In Vitro and In Vivo
Proceedings of the ATS, August 15, 2008; 5(6): 717 - 722.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Kida, M. L. Mucenski, A. R. Thitoff, T. D. Le Cras, K.-S. Park, M. Ikegami, W. Muller, and J. A. Whitsett
GP130-STAT3 Regulates Epithelial Cell Migration and Is Required for Repair of the Bronchiolar Epithelium
Am. J. Pathol., June 1, 2008; 172(6): 1542 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. Jakiela, R. Brockman-Schneider, S. Amineva, W.-M. Lee, and J. E. Gern
Basal Cells of Differentiated Bronchial Epithelium Are More Susceptible to Rhinovirus Infection
Am. J. Respir. Cell Mol. Biol., May 1, 2008; 38(5): 517 - 523.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
P. Chen, J. K. McGuire, R. C. Hackman, K.-H. Kim, R. A. Black, K. Poindexter, W. Yan, P. Liu, A. J. Chen, W. C. Parks, et al.
Tissue Inhibitor of Metalloproteinase-1 Moderates Airway Re-Epithelialization by Regulating Matrilysin Activity
Am. J. Pathol., May 1, 2008; 172(5): 1256 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
V. Dave, S. E. Wert, T. Tanner, A. R. Thitoff, D. E. Loudy, and J. A. Whitsett
Conditional Deletion of Pten Causes Bronchiolar Hyperplasia
Am. J. Respir. Cell Mol. Biol., March 1, 2008; 38(3): 337 - 345.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. F. Kim
Paving the road for lung stem cell biology: bronchioalveolar stem cells and other putative distal lung stem cells
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1092 - L1098.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. P. Wong, A. E. Dutly, A. Sacher, H. Lee, D. M. Hwang, M. Liu, S. Keshavjee, J. Hu, and T. K. Waddell
Targeted cell replacement with bone marrow cells for airway epithelial regeneration
Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L740 - L752.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. R. Loebinger and S. M. Janes
Stem Cells for Lung Disease
Chest, July 1, 2007; 132(1): 279 - 285.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
D. Angeloni
Molecular analysis of deletions in human chromosome 3p21 and the role of resident cancer genes in disease
Briefings in Functional Genomics, May 24, 2007; (2007) elm007v1.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. Heguy, B.-G. Harvey, P. L. Leopold, I. Dolgalev, T. Raman, and R. G. Crystal
Responses of the human airway epithelium transcriptome to in vivo injury
Physiol Genomics, April 24, 2007; 29(2): 139 - 148.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. D. Reynolds, H. Shen, P. R. Reynolds, T. Betsuyaku, J. M. Pilewski, F. Gambelli, M. DeGuiseppe, L. A. Ortiz, and B. R. Stripp
Molecular and functional properties of lung SP cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L972 - L983.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Giangreco, K. R. Groot, and S. M. Janes
Lung Cancer and Lung Stem Cells: Strange Bedfellows?
Am. J. Respir. Crit. Care Med., March 15, 2007; 175(6): 547 - 553.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. L. Rawlins, L. E. Ostrowski, S. H. Randell, and B. L. M. Hogan
Inaugural Article: Lung development and repair: Contribution of the ciliated lineage
PNAS, January 9, 2007; 104(2): 410 - 417.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Estrach, C. A. Ambler, C. L. Lo Celso, K. Hozumi, and F. M. Watt
Jagged 1 is a {beta}-catenin target gene required for ectopic hair follicle formation in adult epidermis
Development, November 15, 2006; 133(22): 4427 - 4438.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. H. Randell
Airway Epithelial Stem Cells and the Pathophysiology of Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2006; 3(8): 718 - 725.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Tian, R. Mahmood, R. Hnasko, and J. Locker
Loss of Nkx2.8 Deregulates Progenitor Cells in the Large Airways and Leads to Dysplasia
Cancer Res., November 1, 2006; 66(21): 10399 - 10407.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
R. J. Homer, Z. Zhu, L. Cohn, C. G. Lee, W. I. White, S. Chen, and J. A. Elias
Differential expression of chitinases identify subsets of murine airway epithelial cells in allergic inflammation
Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L502 - L511.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Ibricevic, A. Pekosz, M. J. Walter, C. Newby, J. T. Battaile, E. G. Brown, M. J. Holtzman, and S. L. Brody
Influenza Virus Receptor Specificity and Cell Tropism in Mouse and Human Airway Epithelial Cells
J. Virol., August 1, 2006; 80(15): 7469 - 7480.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. L. Rawlins and B. L. M. Hogan
Epithelial stem cells of the lung: privileged few or opportunities for many?
Development, July 1, 2006; 133(13): 2455 - 2465.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T.-Y. Ling, M.-D. Kuo, C.-L. Li, A. L. Yu, Y.-H. Huang, T.-J. Wu, Y.-C. Lin, S.-H. Chen, and J. Yu
Identification of pulmonary Oct-4+ stem/progenitor cells and demonstration of their susceptibility to SARS coronavirus (SARS-CoV) infection in vitro
PNAS, June 20, 2006; 103(25): 9530 - 9535.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
D. J. Weiss, M. A. Berberich, Z. Borok, D. B. Gail, J. K. Kolls, C. Penland, and D. J. Prockop
Adult Stem Cells, Lung Biology, and Lung Disease
Proceedings of the ATS, May 1, 2006; 3(3): 193 - 207.
[Full Text] [PDF]

Home page
 J. Immunol.Home page
B. N. Gomperts, J. A. Belperio, P. N. Rao, S. H. Randell, M. C. Fishbein, M. D. Burdick, and R. M. Strieter
Circulating Progenitor Epithelial Cells Traffic via CXCR4/CXCL12 in Response to Airway Injury
J. Immunol., February 1, 2006; 176(3): 1916 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. Loi, T. Beckett, K. K. Goncz, B. T. Suratt, and D. J. Weiss
Limited Restoration of Cystic Fibrosis Lung Epithelium In Vivo with Adult Bone Marrow-derived Cells
Am. J. Respir. Crit. Care Med., January 15, 2006; 173(2): 171 - 179.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
C. Coraux, R. Hajj, P. Lesimple, and E. Puchelle
In vivo models of human airway epithelium repair and regeneration
Eur. Respir. Rev., December 1, 2005; 14(97): 131 - 136.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. A. Voynow, B. M. Fischer, B. C. Roberts, and A. D. Proia
Basal-like Cells Constitute the Proliferating Cell Population in Cystic Fibrosis Airways
Am. J. Respir. Crit. Care Med., October 15, 2005; 172(8): 1013 - 1018.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. A. Benitah, M. Frye, M. Glogauer, and F. M. Watt
Stem Cell Depletion Through Epidermal Deletion of Rac1
Science, August 5, 2005; 309(5736): 933 - 935.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Petiot, C. L. Perriton, C. Dickson, and M. J. Cohn
Development of the mammalian urethra is controlled by Fgfr2-IIIb
Development, May 15, 2005; 132(10): 2441 - 2450.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Coraux, B. Nawrocki-Raby, J. Hinnrasky, C. Kileztky, D. Gaillard, C. Dani, and E. Puchelle
Embryonic Stem Cells Generate Airway Epithelial Tissue
Am. J. Respir. Cell Mol. Biol., February 1, 2005; 32(2): 87 - 92.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
J. CALBO, R. MEUWISSEN, E. V. MONTFORT, O. V. TELLINGEN, and A. BERNS
Genotype-Phenotype Relationships in a Mouse Model for Human Small-Cell Lung Cancer
Cold Spring Harb Symp Quant Biol, January 1, 2005; 70(0): 225 - 232.
[Abstract] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. M. Evans, O. W. Williams, M. J. Tuvim, R. Nigam, G. P. Mixides, M. R. Blackburn, F. J. DeMayo, A. R. Burns, C. Smith, S. D. Reynolds, et al.
Mucin Is Produced by Clara Cells in the Proximal Airways of Antigen-Challenged Mice
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 382 - 394.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. J. Dubin and J. K. Kolls
Further defining lung SP cells: their origin and their heterogeneity, now if we only knew their fate!
Am J Physiol Lung Cell Mol Physiol, September 1, 2004; 287(3): L475 - L476.
[Full Text] [PDF]

Home page
 ThoraxHome page
D Faratian
Bronchial basal cell and epithelial repair
Thorax, August 1, 2004; 59(8): 693 - 693.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Society for Investigative Pathology.