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(American Journal of Pathology. 2004;164:861-871.)
© 2004 American Society for Investigative Pathology

CD208/Dendritic Cell-Lysosomal Associated Membrane Protein Is a Marker of Normal and Transformed Type II Pneumocytes

Bruno Salaun*, Blandine de Saint-Vis*, Nathalie Pacheco{dagger}, Yves Pacheco{dagger}, Arnaud Riesler{dagger}, Sylvie Isaac{dagger}, Caroline Leroux{ddagger}, Valérie Clair-Moninot*, Jean-Jacques Pin*, Janice Griffith§, Isabelle Treilleux, Sophie Goddard, Jean Davoust||, Monique Kleijmeer§ and Serge Lebecque*{dagger}

From the Laboratory for Immunological Research,*Schering Plough Research Institute, Dardilly, France; the Centre Hospitalier Lyon Sud,{dagger}Pierre-Bénite, France; Unité Mixte de Recherche 754 Rétrovirus et Pathologie Comparée,{ddagger}Institut National de la Recherche Agronomique/ Université Claude Bernard/ Ecole Nationale Vétérinaire de Lyon Université Claude Bernard, Lyon, France; the Department of Cell Biology and Institute of Biomembranes,§Utrecht University School of Medecine, Utrecht, The Netherlands; Department of Pathology,Centre Léon Bérard, Lyon, France and UMR 8115 GENETHON–BP 60,||Evry, France

Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208, a member of the lysosomal associated membrane protein (LAMP) family, is specifically expressed by human DCs on activation. However, its mouse counterpart could not be detected in mature DCs. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B, as well as with intracellular MHC class II molecules that accumulate in the same organelles. Expression of DC-LAMP was also occasionally detected at the cell surface of type II pneumocytes. Interestingly, human bronchioloalveolar carcinoma tumor cells, which correspond to transformed type II pneumocytes, express DC-LAMP. Similar observations were made in the Jaagsiekte sheep retrovirus-associated ovine pulmonary adenocarcinoma, a model of human bronchioloalveolar carcinoma. This study establishes that DC-LAMP is constitutively expressed in normal type II pneumocytes. Furthermore, DC-LAMP appears to be a marker of transformed type II pneumocytes as well, an observation that may help the study and the classification of human lung adenocarcinomas.




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