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(American Journal of Pathology. 2004;164:975-985.)
© 2004 American Society for Investigative Pathology

Intracellular Accumulation of Amyloidogenic Fragments of Amyloid-ß Precursor Protein in Neurons with Niemann-Pick Type C Defects Is Associated with Endosomal Abnormalities

Lee-Way Jin*, Izumi Maezawa*, Inez Vincent* and Thomas Bird{dagger}

From the Departments of Pathology*and Neurology,{dagger}University of Washington, Seattle, Washington

Niemann-Pick type C disease (NPC) is characterized by neurodegeneration secondary to impaired cholesterol trafficking and excessive glycosphingolipid storage. Abnormal cholesterol and ganglioside metabolism may influence the generation and aggregation of amyloidogenic fragments (ie, C99 and Aß) from amyloid-ß precursor protein (APP), crucial factors causing neurodegeneration in Alzheimer’s disease. To reveal whether abnormal accumulation and aggregation of APP fragments also occurs in NPC, we studied their expression in cultured cortical neurons treated with U18666A, a compound widely used to induce NPC defects, and also in brain tissues from NPC patients. U18666A treatment resulted in increased intraneuronal levels of C99 and insoluble Aß42, which were distributed among early and late endosomes, in compartments distinct from where endogenous cholesterol accumulates. Analyses of NPC brains revealed that C99 or other APP C-terminal fragments (APP-CTF), but not Aß42, accumulated in Purkinje cells, mainly in early endosomes. In contrast, in hippocampal pyramidal neurons, the major accumulated species was Aß42, in late endosomes. Similar to what has been shown in Alzheimer’s disease, cathepsin D, a lysosomal hydrolase, was redistributed to early endosomes in NPC Purkinje cells, where it co-localized with C99/APP-CTF. Our results suggest that endosomal abnormalities related to abnormal lipid trafficking in NPC may contribute to abnormal APP processing and Aß42/C99/APP-CTF deposition.




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