This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hanamoto, H. Articles by Yoshie, O. Search for Related Content PubMed PubMed Citation Articles by Hanamoto, H. Articles by Yoshie, O.

(American Journal of Pathology. 2004;164:997-1006.)
© 2004 American Society for Investigative Pathology

Expression of CCL28 by Reed-Sternberg Cells Defines a Major Subtype of Classical Hodgkin’s Disease with Frequent Infiltration of Eosinophils and/or Plasma Cells

Hitoshi Hanamoto^*, Takashi Nakayama, Hajime Miyazato^*, Sumio Takegawa, Kunio Hieshima, Yoichi Tatsumi^*, Akihisa Kanamaru^* and Osamu Yoshie

From the Department of Hematology, Nephrology, and Rheumatology,^* and the Department of Microbiology, Kinki University School of Medicine, Osaka, Japan

Classical Hodgkin’s disease (HD) is characterized by rare neoplastic Hodgkin and Reed-Sternberg (H-RS) cells within abundant reactive cellular backgrounds. In most cases, H-RS cells originate from the B-cell lineage, but their immunophenotypes are unusual. Here we newly found frequent expression of chemokine receptors CXCR6 and CCR10 and their respective ligands CXCL16 and CCL28 in HD-derived cell lines. CCR10 is known to be selectively expressed by plasma cells, whereas CCL28 attracts eosinophils via CCR3 and plasma cells via CCR10 and CCR3. Therefore, we examined their expression in HD tissues by immunohistochemistry. We found that H-RS cells in 15 of 19 cases were positive for CCL28. Among them, seven cases were also positive for CCR10, suggesting a potential autocrine effect. In situ hybridization confirmed the expression of CCL28 mRNA in H-RS cells. The CCL28 positivity in H-RS cells did not significantly correlate with that of LMP-1, CCL17, CCL22, or CCL11. However, it significantly correlated with the background accumulation of eosinophils, plasma cells, and CCR10⁺ cells. Thus, the production of CCL28 by H-RS cells may play a major role in tissue accumulation of eosinophils and/or plasma cells in classical HD. The frequent expression of CCR10 in H-RS cells themselves also supports their close relationship to plasma cells.

This article has been cited by other articles:

				A. Navarro, T. Diaz, A. Martinez, A. Gaya, A. Pons, B. Gel, C. Codony, G. Ferrer, C. Martinez, E. Montserrat, et al. Regulation of JAK2 by miR-135a: prognostic impact in classic Hodgkin lymphoma Blood, October 1, 2009; 114(14): 2945 - 2951. [Abstract] [Full Text] [PDF]

				J. Wang, Y. Lu, J. Wang, A. E. Koch, J. Zhang, and R. S. Taichman CXCR6 Induces Prostate Cancer Progression by the AKT/Mammalian Target of Rapamycin Signaling Pathway Cancer Res., December 15, 2008; 68(24): 10367 - 10377. [Abstract] [Full Text] [PDF]

				O. Morteau, C. Gerard, B. Lu, S. Ghiran, M. Rits, Y. Fujiwara, Y. Law, K. Distelhorst, E. M. Nielsen, E. D. Hill, et al. An Indispensable Role for the Chemokine Receptor CCR10 in IgA Antibody-Secreting Cell Accumulation J. Immunol., November 1, 2008; 181(9): 6309 - 6315. [Abstract] [Full Text] [PDF]

				Y. Lu, J. Wang, Y. Xu, A. E. Koch, Z. Cai, X. Chen, D. L. Galson, R. S. Taichman, and J. Zhang CXCL16 Functions as a Novel Chemotactic Factor for Prostate Cancer Cells In vitro Mol. Cancer Res., April 1, 2008; 6(4): 546 - 554. [Abstract] [Full Text] [PDF]

				D. Re, R. Kuppers, and V. Diehl Molecular Pathogenesis of Hodgkin's Lymphoma J. Clin. Oncol., September 10, 2005; 23(26): 6379 - 6386. [Abstract] [Full Text] [PDF]