help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jensen, C. H.
Right arrow Articles by Bisgaard, H. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jensen, C. H.
Right arrow Articles by Bisgaard, H. C.
(American Journal of Pathology. 2004;164:1347-1359.)
© 2004 American Society for Investigative Pathology

Transit-Amplifying Ductular (Oval) Cells and Their Hepatocytic Progeny Are Characterized by a Novel and Distinctive Expression of Delta-Like Protein/Preadipocyte Factor 1/Fetal Antigen 1

Charlotte Harken Jensen*, Eva Irene Jauho{dagger}, Eric Santoni-Rugiu{ddagger}, Uffe Holmskov*, Børge Teisner*, Niels Tygstrup§ and Hanne Cathrine Bisgaard{dagger}

From the Department of Immunology and Microbiology,* Danish Stem Cell Research Center, University of Southern Denmark, Odense; the Department of Medical Biochemistry and Genetics,{dagger} The Panum Institute, University of Copenhagen, Copenhagen; and the Departments of Pathology{ddagger} and Hepatology,§ Rigshospitalet, Copenhagen, Denmark

Hepatic regeneration from toxic or surgical injury to the adult mammalian liver, endorses different cellular responses within the hepatic lineage. The molecular mechanisms determining commitment of a cell population at a specific lineage level to participate in liver repair as well as the fate of its progeny in the hostile environment created by the injury are not well defined. Based on the role of the Notch/Delta/Jagged system in cell fate specification and recent reports linking Notch signaling with normal bile duct formation in mouse and human liver, we examined the expression of Notch1, Notch2, Notch3, Delta1, Delta3, Jagged1, and Jagged2, and delta-like protein/preadipocyte factor 1/fetal antigen 1 (dlk) in four well-defined experimental rat models of liver injury and regeneration. Although Delta3 and Jagged2 were undetectable by reverse transcriptase-polymerase chain reaction and Northern blot, we observed the most significant up-regulation of all other transcripts in the 2-acetylaminofluorene-70% hepatectomy (AAF/PHx) model, in which liver mass is restored by proliferation and differentiation of transit-amplifying ductular (oval) cells. The most profound change was observed for dlk. Accordingly, immunohistochemical analyses in the AAF/PHx model showed a specific expression of dlk in atypical ductular structures composed of oval cells. Delta-like protein was not observed in proliferating hepatocytes or bile duct cells after partial hepatectomy or ligation of the common bile duct whereas clusters of dlk immunoreactive oval cells were found in both the retrorsine and the AAF/PHx models. Finally, we used dlk to isolate {alpha}-fetoprotein-positive cells from fetal and adult regenerating rat liver by a novel antibody panning technique.




This article has been cited by other articles:


Home page
 Circ. Res.Home page
N. A. Gude, G. Emmanuel, W. Wu, C. T. Cottage, K. Fischer, P. Quijada, J. A. Muraski, R. Alvarez, M. Rubio, E. Schaefer, et al.
Activation of Notch-Mediated Protective Signaling in the Myocardium
Circ. Res., May 9, 2008; 102(9): 1025 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
S. A. Ochsner, H. Strick-Marchand, Q. Qiu, S. Venable, A. Dean, M. Wilde, M. C. Weiss, and G. J. Darlington
Transcriptional Profiling of Bipotential Embryonic Liver Cells to Identify Liver Progenitor Cell Surface Markers
Stem Cells, October 1, 2007; 25(10): 2476 - 2487.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. M. Abdallah, M. Ding, C. H. Jensen, N. Ditzel, A. Flyvbjerg, T. G. Jensen, F. Dagnaes-Hansen, J. A. Gasser, and M. Kassem
Dlk1/FA1 Is a Novel Endocrine Regulator of Bone and Fat Mass and Its Serum Level Is Modulated by Growth Hormone
Endocrinology, July 1, 2007; 148(7): 3111 - 3121.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. Huang, X. Zhang, M. Zhang, J.-D. Zhu, Y.-L. Zhang, Y. Lin, K.-S. Wang, X.-F. Qi, Q. Zhang, G.-Z. Liu, et al.
Up-regulation of DLK1 as an imprinted gene could contribute to human hepatocellular carcinoma
Carcinogenesis, May 1, 2007; 28(5): 1094 - 1103.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. M. Abdallah, P. Boissy, Q. Tan, J. Dahlgaard, G. A. Traustadottir, K. Kupisiewicz, J. Laborda, J.-M. Delaisse, and M. Kassem
dlk1/FA1 Regulates the Function of Human Bone Marrow Mesenchymal Stem Cells by Modulating Gene Expression of Pro-inflammatory Cytokines and Immune Response-related Factors
J. Biol. Chem., March 9, 2007; 282(10): 7339 - 7351.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Nishikawa, Y. Doi, H. Watanabe, T. Tokairin, Y. Omori, M. Su, T. Yoshioka, and K. Enomoto
Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro
Am. J. Pathol., April 1, 2005; 166(4): 1077 - 1088.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Society for Investigative Pathology.