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(American Journal of Pathology. 2004;164:1849-1856.)
© 2004 American Society for Investigative Pathology

Electrophysiological Properties of the Airway

Epithelium in the Murine, Ovalbumin Model of Allergic Airway Disease

Michelle M. Cloutier^*, Linda Guernsey^*, Carol A. Wu and Roger S. Thrall

From the Departments of Pediatrics^* and Medicine, University of Connecticut Health Center, Farmington, Connecticut

The electrophysiological properties of cultured tracheal cells (CTCs) were examined in a murine (C57BL/6J), ovalbumin (OVA)-induced model of allergic airway disease (AAD) at early (3-day OVA-aerosol) and peak (10-day OVA-aerosol) periods of inflammation. Transepithelial potential difference, short-circuit current (Isc), and resistance (R_T) were lower in CTCs from 10-day OVA-aerosol animals compared to CTCs from naïve mice. In cells cultured for 5 weeks, R_T was greater in naive CTCs than in 10-day OVA-aerosol CTCs at all times (P < 0.01). The Isc response to mucosal amiloride (10^–4 mol/L) was increased in CTCs from 10-day OVA-aerosol mice compared to naïve mice (6.0 ± 0.37 µA/cm² versus 1.8 ± 0.56 µA/cm²; P < 0.001) with intermediate values for CTCs from 3-day OVA-aerosol mice. The cAMP-induced increase in Isc was blunted in 10-day OVA-aerosol animals compared to CTCs from naïve mice (9 ± 12% versus 39 ± 7%; P < 0.01) with intermediate values for CTCs from 3-day OVA-aerosol mice. There was no difference in mannitol flux in naïve compared to 10-day OVA-aerosol CTCs. Similar results were found using intact tracheas mounted in a perfusion chamber. These data demonstrate changes in airway epithelial cell function in the OVA-induced model of AAD that may contribute to the pathogenesis of airway inflammation.

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