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(American Journal of Pathology. 2004;164:2279-2288.)
© 2004 American Society for Investigative Pathology

Male Infertility and DNA Damage in Doppel Knockout and Prion Protein/Doppel Double-Knockout Mice

Derek Paisley*, Stephen Banks{dagger}, Jim Selfridge{ddagger}, Neil F. McLennan*§, Ann-Marie Ritchie*, Carolanne McEwan*, D. Stewart Irvine{dagger}, Philippa T. K. Saunders{dagger}, Jean C. Manson and David W. Melton*

From the Molecular Medicine Centre,*Sir Alastair Currie Cancer Research UK Laboratories, the Medical Research Council Human Reproductive Sciences Unit,{dagger}Centre for Reproductive Biology, the Institute of Cell and Molecular Biology,{ddagger}and the National Creutzfeldt-Jakob Disease Surveillance Unit,§University of Edinburgh, Edinburgh; and the Neuropathogenesis Unit,Institute for Animal Health, Edinburgh, Scotland

The prion protein (PrP) and Doppel (Dpl) have many structural and biochemical properties in common, leading to the suggestion that the lack of an obvious phenotype in PrP-deficient mice maybe because of compensation by Dpl. To test this hypothesis and also investigate the function of Dpl we have generated Prnd–/– and Prnp–/–/Prnd–/– mouse lines. Both develop normally and display an identical male sterility phenotype that differs from that reported for another Prnd–/– mouse line. Sperm from both our mutant lines were present at normal concentrations, had normal motility, and no morphological abnormalities. Despite only rarely fertilizing oocytes in vivo, because of an inability to perform the acrosome reaction, mutant sperm were capable of fertilization in vitro, albeit at reduced rates compared to wild type. Elevated levels of oxidative DNA damage were found in both types of mutant sperm and resulting embryos failed at an early stage. Therefore we found no evidence that Dpl compensates for the loss of PrP function in mutant mouse lines, but it does have an important anti-oxidant function necessary for sperm integrity and male fertility.




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