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(American Journal of Pathology. 2004;165:533-540.)
© 2004 American Society for Investigative Pathology

Phenotypic Switch from Paracrine to Autocrine Role of Hepatocyte Growth Factor in an Androgen-Independent Human Prostatic Carcinoma Cell Line, CWR22R

Koh-ichi Nakashiro^*, Shingo Hara^*, Yuji Shinohara^*, Miho Oyasu, Hitoshi Kawamata, Satoru Shintani^*, Hiroyuki Hamakawa^* and Ryoichi Oyasu

From the Department of Oral and Maxillofacial Surgery,^* Ehime University School of Medicine, Ehime, Japan; the Department of Surgical and Molecular Pathology, Dokkyo University School of Medicine, Tochigi, Japan; and the Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, Illinois

Support mechanisms involved in growth of androgen-independent prostate cancer are primarily unknown. Hepatocyte growth factor (HGF)/Met has been suggested to be one of them based primarily on immunohistochemical studies. We conducted a series of experiments to assess the role of the HGF/Met system in an androgen-dependent human prostate carcinoma, CWR22 and its androgen-independent derivative, CWR22R. We found that action of HGF changed from paracrine to autocrine in progression to androgen-independent state. CWR22 tumors did not express HGF but expressed Met, whereas prostate stromal cells expressed HGF at a high level. Growth of CWR22 was stimulated either by addition of HGF to the culture or by the presence of prostate stromal cells. On the other hand, CWR22R cells expressed both HGF and Met. Knockdown of Met expression by RNA interference method suppressed the growth of CWR22R cells. Our data suggest that HGF is intimately involved in growth of human prostate cancer and that progression from the androgen-dependent to the androgen-independent state is associated with an adaptive switch in support mechanism from paracrine to autocrine. Our data offer one mechanism to account for androgen-independent human cancer growth.

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