help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lippens, S.
Right arrow Articles by Declercq, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lippens, S.
Right arrow Articles by Declercq, W.
(American Journal of Pathology. 2004;165:833-841.)
© 2004 American Society for Investigative Pathology

Vitamin D3 Induces Caspase-14 Expression in Psoriatic Lesions and Enhances Caspase-14 Processing in Organotypic Skin Cultures

Saskia Lippens*, Mark Kockx{dagger}, Geertrui Denecker*, Michiel Knaapen{ddagger}, An Verheyen{dagger}, Ruben Christiaen*, Erwin Tschachler§, Peter Vandenabeele* and Wim Declercq*

From the Department of Molecular Biomedical Research,* Molecular Signaling and Cell Death Unit, Flanders Interuniversity Institute for Biotechnology (VIB) and Ghent University, Zwijnaarde, Belgium; the Department of Pathology,{dagger} Middelheim Hospital, Antwerpen, Belgium; HistoGenex Naamloze Vennootrchap,{ddagger} Edegem, Belgium; and the Department of Dermatology,§ Vienna University Medical School, Wien, Austria

Caspase-14 is a nonapoptotic caspase family member whose expression in the epidermis is confined to the suprabasal layers, which consist of differentiating keratinocytes. Proteolytic activation of this caspase is observed in the later stages of epidermal differentiation. In psoriatic skin, a dramatic decrease in caspase-14 expression in the parakeratotic plugs was observed. Topical treatment of psoriatic lesions with a vitamin D3 analogue resulted in a decrease of the psoriatic phenotype and an increase in caspase-14 expression in the parakeratotic plugs. To investigate whether vitamin D3 directly affects caspase-14 expression levels, we used keratinocyte cell cultures. 1{alpha},25-Dihydroxycholecalciferol, the biologically active form of vitamin D3, increased caspase-14 expression, whereas retinoic acid inhibited it. Moreover, retinoic acid repressed the vitamin D3-induced caspase-14 expression level. In addition, the use of organotypic skin cultures demonstrated that 1{alpha},25-dihydroxycholecalciferol enhanced epidermal differentiation and caspase-14 activation, whereas retinoic acid completely blocked caspase-14 processing. Our data indicate that caspase-14 plays an important role in terminal epidermal differentiation, and its absence may contribute to the psoriatic phenotype.




This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
G. Denecker, P. Ovaere, P. Vandenabeele, and W. Declercq
Caspase-14 reveals its secrets
J. Cell Biol., February 6, 2008; 180(3): 451 - 458.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Krajewska, H. Kim, E. Shin, S. Kennedy, M. J. Duffy, Y. F. Wong, D. Marr, J. Mikolajczyk, A. Shabaik, I. Meinhold-Heerlein, et al.
Tumor-Associated Alterations in Caspase-14 Expression in Epithelial Malignancies
Clin. Cancer Res., August 1, 2005; 11(15): 5462 - 5471.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Society for Investigative Pathology.