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(American Journal of Pathology. 2004;165:1141-1150.)
© 2004 American Society for Investigative Pathology

Deficient Eosinophil Chemotaxis-Promoting Activity of Genetically Normal Mast Cells Transplanted into Subcutaneous Tissue of Mitfmi-vga9/Mitfmi-vga9 Mice

Comparison of the Activity and Mast Cell Distribution Pattern with KitW/KitW-vMice

Keisuke Oboki, Eiichi Morii and Yukihiko Kitamura

From the Department of Pathology, Graduate School of Frontier Bioscience and Medical School, Osaka University, Osaka, Japan

Despite the practical lack of mast cells in the skin tissue of WBB6F1-KitW/KitW-v, the skin tissue of WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice contains one third of mast cells than that of WBB6F1-+/+ mice. We attempted to investigate the function of the decreased but appreciable number of mast cells in the skin of WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice. The substance P (SP)-induced eosinophil infiltration was examined using air-bleb assay. The air-bleb membrane was composed of the subcutaneous connective tissue. Unexpectedly, we found that the air-bleb membranes formed in the back of WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice contained no mast cells. The WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice showed impaired SP-induced eosinophil infiltration as observed in WBB6F1-KitW/KitW-v mice, indicating that mast cells detected in the dermis of WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice did not help SP-induced eosinophil infiltration. Subcutaneous transplantation of cultured mast cells from WBB6F1-+/+ mice normalized SP-induced eosinophil infiltration in WBB6F1-KitW/KitW-v mice but not in WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice. The greater number and the more dispersed distribution pattern of mast cells that appeared in the subcutaneous connective tissue of WBB6F1-KitW/KitW-v mice after the transplantation appeared to explain the difference between WBB6F1-KitW/KitW-v and WBB6F1-Mitfmi-vga9/Mitfmi-vga9 mice.






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Copyright © 2004 by the American Society for Investigative Pathology.