help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gerlini, G.
Right arrow Articles by Nestle, F. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gerlini, G.
Right arrow Articles by Nestle, F. O.
(American Journal of Pathology. 2004;165:1853-1863.)
© 2004 American Society for Investigative Pathology

Metastatic Melanoma Secreted IL-10 Down-Regulates CD1 Molecules on Dendritic Cells in Metastatic Tumor Lesions

Gianni Gerlini*, Adrian Tun-Kyi{dagger}, Christa Dudli{dagger}, Günter Burg{dagger}, Nicola Pimpinelli* and Frank O. Nestle{dagger}

From the Department of Dermatology,{dagger} University of Zurich Medical School, Zurich, Switzerland; and the Department of Dermatological Sciences,* University of Florence Medical School, Florence, Italy

CD1 molecules are expressed by antigen-presenting cells such as dendritic cells and mediate primary immune responses to lipids and glycolipids which have been shown to be expressed by various tumors. Glycolipids are expressed by melanoma cells but, despite their immunogenicity, no efficient spontaneous immune responses are elicited. As IL-10 has previously been shown to down-regulate CD1a on dendritic cells and is known to be expressed by various melanoma cell lines, we investigated if melanoma-derived IL-10 could down-regulate CD1 molecule expression on dendritic cells as a possible way to circumvent immune recognition. We found that CD1a, CD1b, CD1c, and CD1d were significantly down-regulated on dendritic cells in metastatic (n = 10) but not in primary melanoma lesions (n = 10). We further detected significantly higher IL-10 protein levels in metastatic than in primary melanomas. Moreover, supernatants from metastatic melanomas were significantly more effective in down-regulating CD1 molecules on dendritic cells than supernatants from primary melanoma cultures. This effect was blocked using a neutralizing IL-10 antibody in a dose dependent manner. Our findings suggest that metastatic but not primary melanomas can down-regulate CD1 molecules on infiltrating dendritic cells by secreting IL-10 which may represent a novel way to escape the immune response directed against the tumor.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Yuan, X. Qi, P. Tsang, S.-J. Kang, P. A. Illarionov, G. S. Besra, J. Gumperz, and P. Cresswell
Saposin B is the dominant saposin that facilitates lipid binding to human CD1d molecules
PNAS, March 27, 2007; 104(13): 5551 - 5556.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. R. Zubieta, D. Furman, M. Barrio, A. I. Bravo, E. Domenichini, and J. Mordoh
Galectin-3 Expression Correlates with Apoptosis of Tumor-Associated Lymphocytes in Human Melanoma Biopsies
Am. J. Pathol., May 1, 2006; 168(5): 1666 - 1675.
[Abstract] [Full Text] [PDF]

Home page
 Journal Watch DermatologyHome page
Metastatic Malignant Melanoma Creatively Avoids Immune Surveillance
Journal Watch Dermatology, February 2, 2005; 2005(202): 3 - 3.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Society for Investigative Pathology.