| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Laboratori de Neuropatología Molecular,* Departament de Ciències Mèdiques Bàsiques, Universitat de Lleida, Lleida, Spain; the Institut de Neuropatologia, Servei d’Anatomia Patològica, Hospital de Bellvitge, Universitat de Barcelona, Hospitalet de Llobregat, Barcelona, Spain; the Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, New York, New York; and the Division of Hematology, Department of Medicine, Weill Medical College of Cornell University, New York, New York
The pro-form of nerve growth factor (pro-NGF) has been shown to be a high affinity ligand for p75NTR and to induce apoptosis through this receptor. It has been reported that pro-NGF, rather than mature NGF, is the predominant form of this neurotrophin in human brain. In the present work we studied the potential involvement of pro-NGF purified from human brains affected by Alzheimer’s disease (AD), where it is especially abundant, in the neuronal apoptosis observed in this disease. Western blot analysis of human brain tissue showed the existence of several pro-NGF forms. Some of these pro-NGF forms were significantly increased in AD brain cortex in a disease stage-dependent manner. Pro-NGF, purified by chromatography from human AD brains, induced apoptotic cell death in sympathetic neurons and in a p75NTR stably transfected cell line. Blocking p75NTR in cell culture abolished neuronal apoptosis caused by pro-NGF. p75NTR-transfected cells underwent apoptosis in the presence of pro-NGF while control wild-type cells did not. Taken together, these results indicate that pro-NGF purified from AD human brains can induce apoptosis in neuronal cell cultures through its interaction with the p75NTR receptor.
This article has been cited by other articles:
 |
|
 |
|
  F. Ferraro, X.-M. Ma, J. A. Sobota, B. A. Eipper, and R. E. Mains Kalirin/Trio Rho Guanine Nucleotide Exchange Factors Regulate a Novel Step in Secretory Granule Maturation Mol. Biol. Cell, December 1, 2007; 18(12): 4813 - 4825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. AL-SHAWI, A. HAFNER, S. CHUN, S. RAZA, K. CRUTCHER, C. THRASIVOULOU, P. SIMONS, and T. COWEN ProNGF, Sortilin, and Age-related Neurodegeneration Ann. N.Y. Acad. Sci., November 1, 2007; 1119(1): 208 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kumar, R. A. Sinha, M. Tiwari, L. Pal, A. Shrivastava, R. Singh, K. Kumar, S. Kumar Gupta, and M. M. Godbole Increased Pro-Nerve Growth Factor and p75 Neurotrophin Receptor Levels in Developing Hypothyroid Rat Cerebral Cortex Are Associated with Enhanced Apoptosis Endocrinology, October 1, 2006; 147(10): 4893 - 4903. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tan and R. K. Shepherd Aminoglycoside-Induced Degeneration of Adult Spiral Ganglion Neurons Involves Differential Modulation of Tyrosine Kinase B and p75 Neurotrophin Receptor Signaling Am. J. Pathol., August 1, 2006; 169(2): 528 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Podlesniy, A. Kichev, C. Pedraza, J. Saurat, M. Encinas, B. Perez, I. Ferrer, and C. Espinet Pro-NGF from Alzheimer's Disease and Normal Human Brain Displays Distinctive Abilities to Induce Processing and Nuclear Translocation of Intracellular Domain of p75NTR and Apoptosis Am. J. Pathol., July 1, 2006; 169(1): 119 - 131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. K. Teng, K. K. Teng, R. Lee, S. Wright, S. Tevar, R. D. Almeida, P. Kermani, R. Torkin, Z.-Y. Chen, F. S. Lee, et al. ProBDNF Induces Neuronal Apoptosis via Activation of a Receptor Complex of p75NTR and Sortilin J. Neurosci., June 1, 2005; 25(22): 5455 - 5463. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
