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(American Journal of Pathology. 2005;166:811-822.)
© 2005 American Society for Investigative Pathology

Abnormal T-Cell Reactivity against Paternal Antigens in Spontaneous Abortion

Adoptive Transfer of Pregnancy-Induced CD4+CD25+ T Regulatory Cells Prevents Fetal Rejection in a Murine Abortion Model

Ana Claudia Zenclussen, Katrin Gerlof, Maria Laura Zenclussen, André Sollwedel, Annarosa Zambon Bertoja, Thomas Ritter, Katja Kotsch, Joachim Leber and Hans-Dieter Volk

From the Institute of Medical Immunology, Charité, Medical University Berlin, Berlin, Germany

Mammalian pregnancy is thought to be a state of immunological tolerance. The mechanisms underlying this phenomenon are still poorly understood. Here, we determined whether an inappropriate function of T regulatory (Treg) cells is involved in the pathogenesis of spontaneous abortion. We evaluated spleen and decidual lymphocytes from CBA/J mice undergoing immunological abortion (DBA/2J-mated) or having normal pregnancy (BALB/c-mated) on day 14 of gestation for ex vivo cytokine production after PMA or paternal antigen (alloantigen) stimulation. Treg activity was characterized by quantifying CD4+CD25+ cells, foxp3 expression, and interleukin-10 secretion. Decidual lymphocytes from abortion CBA/J mice contained a significantly higher frequency of interferon-{gamma}-producing T cells specific for paternal antigens compared to those from normal pregnancy (7.8% versus 2.7%, P < 0.05). Compared to virgin CBA/J females, normal pregnant mice showed strongly elevated numbers of CD4+CD25+ and interleukin-10+ Treg cells in the thymus whereas significantly lower frequencies of Treg cells were observed in abortion mice. Very interestingly, CD4+CD25+ Treg cells from normal pregnant and nonpregnant CBA/J mice could inhibit both proliferation and interferon-{gamma} secretion of lymphocytes from abortion mice in vitro whereas in vivo prevention of fetal rejection could only be achieved after adoptive transfer of Treg cells from normal pregnant mice. Our data suggest that pregnancy-induced Treg cells play a vital role in maternal tolerance to the allogeneic fetus.




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