help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lügering, A.
Right arrow Articles by Kucharzik, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lügering, A.
Right arrow Articles by Kucharzik, T.
(American Journal of Pathology. 2005;166:1647-1654.)
© 2005 American Society for Investigative Pathology

Absence of CCR6 Inhibits CD4+ Regulatory T-Cell Development and M-Cell Formation inside Peyer’s Patches

Andreas Lügering*, Martin Floer*, Sabine Westphal*, Christian Maaser*, Thomas W. Spahn*, M. Alexander Schmidt{dagger}, Wolfram Domschke*, Ifor R. Williams{ddagger} and Torsten Kucharzik*

From the Departments of Medicine B* and Infectiology,{dagger} University of Münster, Münster, Germany; and the Departments of Pathology and Laboratory Medicine and of Dermatology,{ddagger} Emory University School of Medicine, Atlanta, Georgia

The chemokine Mip3{alpha} is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer’s patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6. Although CCR6-deficient mice are known to have a perturbed intestinal immune system, little is known about the specific impact of this interaction for Peyer’s patch formation. To elucidate the effect of Mip3{alpha} on PP lymphocyte development, we used a CCR6/enhanced green fluorescent protein (EGFP) knock-in mouse model and analyzed lymphocyte development by immunohistochemistry and flow cytometry. PPs of CCR6–/– mice were significantly size-reduced with a proportional loss of B cells and T cells, whereas T-cell subsets were disturbed with a decreased CD4/CD8 ratio paralleled with a loss of regulatory CD4+ CD45Rblow T cells. The analysis of cytokine production by CCR6-expressing cells could demonstrate that CCR6 is involved in the regulation of cytokine secretion such as interleukin-12 by dendritic cells. Quantification of UEA-1+ cells inside the FAE showed reduced M-cell numbers in CCR6-deficient mice. These results suggest that the interaction of CCR6 with its ligand Mip3{alpha} is important for immune responses generated inside the PPs, particularly for the generation of regulatory CD4+ T cells residing inside PPs and for the formation of M cells.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
J. Ochoa-Reparaz, A. Rynda, M. A. Ascon, X. Yang, I. Kochetkova, C. Riccardi, G. Callis, T. Trunkle, and D. W. Pascual
IL-13 Production by Regulatory T Cells Protects against Experimental Autoimmune Encephalomyelitis Independently of Autoantigen
J. Immunol., July 15, 2008; 181(2): 954 - 968.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Westphal, A. Lugering, J. von Wedel, C. von Eiff, C. Maaser, T. Spahn, G. Heusipp, M. A. Schmidt, H. Herbst, I. R. Williams, et al.
Resistance of Chemokine Receptor 6-Deficient Mice to Yersinia Enterocolitica Infection: Evidence of Defective M-Cell Formation in Vivo
Am. J. Pathol., March 1, 2008; 172(3): 671 - 680.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. W. Lim, J. Lee, P. Hillsamer, and C. H. Kim
Human Th17 Cells Share Major Trafficking Receptors with Both Polarized Effector T Cells and FOXP3+ Regulatory T Cells
J. Immunol., January 1, 2008; 180(1): 122 - 129.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. P. Phadke, G. Akangire, S. J. Park, S. A. Lira, and B. Mehrad
The Role of CC Chemokine Receptor 6 in Host Defense in a Model of Invasive Pulmonary Aspergillosis
Am. J. Respir. Crit. Care Med., June 1, 2007; 175(11): 1165 - 1172.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. G. McDonald, J. S. McDonough, C. Wang, T. Kucharzik, I. R. Williams, and R. D. Newberry
CC Chemokine Receptor 6 Expression by B Lymphocytes Is Essential for the Development of Isolated Lymphoid Follicles
Am. J. Pathol., April 1, 2007; 170(4): 1229 - 1240.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. Plancoulaine, A. Gessain, P. Tortevoye, A. Boland-Auge, A. Vasilescu, F. Matsuda, and L. Abel
A major susceptibility locus for HTLV-1 infection in childhood maps to chromosome 6q27
Hum. Mol. Genet., November 15, 2006; 15(22): 3306 - 3312.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
I. R WILLIAMS
CCR6 and CCL20: Partners in Intestinal Immunity and Lymphorganogenesis.
Ann. N.Y. Acad. Sci., August 1, 2006; 1072: 52 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
A. LUGERING and T. KUCHARZIK
Induction of Intestinal Lymphoid Tissue: The Role of Cryptopatches.
Ann. N.Y. Acad. Sci., August 1, 2006; 1072: 210 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
E. GULLBERG and J. D SODERHOLM
Peyer's Patches and M Cells as Potential Sites of the Inflammatory Onset in Crohn's Disease.
Ann. N.Y. Acad. Sci., August 1, 2006; 1072: 218 - 232.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Hase, T. Murakami, H. Takatsu, T. Shimaoka, M. Iimura, K. Hamura, K. Kawano, S. Ohshima, R. Chihara, K. Itoh, et al.
The Membrane-Bound Chemokine CXCL16 Expressed on Follicle-Associated Epithelium and M Cells Mediates Lympho-Epithelial Interaction in GALT
J. Immunol., January 1, 2006; 176(1): 43 - 51.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2005 by the American Society for Investigative Pathology.