| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Department of Pathology, Immunology, and Laboratory Medicine, and Program in Stem Cell Biology and Regenerative Medicine, College of Medicine, University of Florida, Gainesville, Florida
Embryonic stem (ES) cells have been proposed to be a powerful tool in the study of pancreatic disease, as well as a potential source for cell replacement therapy in the treatment of diabetes. However, data demonstrating the feasibility of using pancreatic islet-like cells differentiated from ES cells remain controversial. In this study we characterized ES cell-derived insulin-expressing cells and assessed their suitability for the treatment of type I diabetes. ES cell-derived insulin-stained cell clusters expressed insulin mRNA and transcription factors associated with pancreatic development. The majority of insulin-positive cells in the clusters also showed immunoreactivity for C-peptide. Insulin was stored in the cytoplasm and released into the culture medium in a glucose-dependent manner. When the cultured cells were transplanted into diabetic mice, they reversed the hyperglycemic state for 
3 weeks, but the rescue failed due to immature teratoma formation. Our studies demonstrate that reversal of hyperglycemia by transplantation of ES cell-derived insulin-producing cells is possible. However, the risk of teratoma formation would need to be eliminated before ES cell-based therapies for the treatment of diabetes are considered.
This article has been cited by other articles:
 |
|
 |
|
  P. S. Knoepfler Deconstructing Stem Cell Tumorigenicity: A Roadmap to Safe Regenerative Medicine Stem Cells, May 1, 2009; 27(5): 1050 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Condic and M. Rao Regulatory Issues for Personalized Pluripotent Cells Stem Cells, November 1, 2008; 26(11): 2753 - 2758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Drobinskaya, T. Linn, T. Saric, R. G. Bretzel, H. Bohlen, J. Hescheler, and E. Kolossov Scalable Selection of Hepatocyte- and Hepatocyte Precursor-Like Cells from Culture of Differentiating Transgenically Modified Murine Embryonic Stem Cells Stem Cells, September 1, 2008; 26(9): 2245 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Zhao, S. A Amiel, M. R Christie, P. Muiesan, P. Srinivasan, W. Littlejohn, M. Rela, M. Arno, N. Heaton, and G. C. Huang Evidence for the presence of stem cell-like progenitor cells in human adult pancreas J. Endocrinol., December 1, 2007; 195(3): 407 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takenaga, M. Fukumoto, and Y. Hori Regulated Nodal signaling promotes differentiation of the definitive endoderm and mesoderm from ES cells J. Cell Sci., June 15, 2007; 120(12): 2078 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M Ackermann and M. Gannon Molecular regulation of pancreatic {beta}-cell mass development, maintenance, and expansion J. Mol. Endocrinol., February 1, 2007; 38(2): 193 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. H. Lee, M. J. Seo, R. L. Reger, J. L. Spees, A. A. Pulin, S. D. Olson, and D. J. Prockop Multipotent stromal cells from human marrow home to and promote repair of pancreatic islets and renal glomeruli in diabetic NOD/scid mice PNAS, November 14, 2006; 103(46): 17438 - 17443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mimeault and S. K. Batra Concise Review: Recent Advances on the Significance of Stem Cells in Tissue Regeneration and Cancer Therapies Stem Cells, November 1, 2006; 24(11): 2319 - 2345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Koch, C. E. Jordan, and J. L. Platt Complement-Dependent Control of Teratoma Formation by Embryonic Stem Cells J. Immunol., October 1, 2006; 177(7): 4803 - 4809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. J. Hall, P. Stojkovic, and M. Stojkovic Using Therapeutic Cloning to Fight Human Disease: A Conundrum or Reality? Stem Cells, July 1, 2006; 24(7): 1628 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Gordon, N. Levicar, M. Pai, P. Bachellier, I. Dimarakis, F. Al-Allaf, H. M'Hamdi, T. Thalji, J. P. Welsh, S. B. Marley, et al. Characterization and Clinical Application of Human CD34+ Stem/Progenitor Cell Populations Mobilized into the Blood by Granulocyte Colony-Stimulating Factor Stem Cells, July 1, 2006; 24(7): 1822 - 1830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shibata, N. Ageyama, Y. Tanaka, Y. Kishi, K. Sasaki, S. Nakamura, S.-i. Muramatsu, S. Hayashi, Y. Kitano, K. Terao, et al. Improved Safety of Hematopoietic Transplantation with Monkey Embryonic Stem Cells in the Allogeneic Setting Stem Cells, June 1, 2006; 24(6): 1450 - 1457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Murry, H. Reinecke, and L. M. Pabon Regeneration Gaps: Observations on Stem Cells and Cardiac Repair J. Am. Coll. Cardiol., May 2, 2006; 47(9): 1777 - 1785. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
