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(American Journal of Pathology. 2005;167:233-242.)
© 2005 American Society for Investigative Pathology

The Loss of Sarco/Endoplasmic Reticulum Calcium Transport ATPase 3 Expression Is an Early Event during the Multistep Process of Colon Carcinogenesis

Jean-Philippe Brouland*, Pascal Gélébart{dagger}, Tünde Kovàcs{ddagger}, Jocelyne Enouf{dagger}, Johannes Grossmann§ and Béla Papp{dagger}

From the Service d’Anatomie et Cytologie Pathologiques* et Inserm U 348,{dagger} Hôpital Lariboisière, Paris, France; the National Medical Center,{ddagger} Institute of Haematology and Immunology, Budapest, Hungary; the Klinik und Poliklinik für Innere Medizin I,§ Klinikum Universität Regensburg, Regensburg, Germany; and the Inserm U-718/EMI-00-03 Laboratoire de Biologie Cellulaire Hématopoïétique, Institut Universitaire d’Hématologie, Hôpital Saint-Louis, Paris, France

Calcium accumulation in the endoplasmic reticulum is accomplished by sarco/endoplasmic reticulum calcium transport ATPases (SERCA enzymes). To better characterize the role of SERCA3 in colon carcinogenesis, its expression has been investigated in colonic epithelium, benign lesions, adenomas, and adenocarcinomas. In addition, the regulation of SERCA3 expression was analyzed in the context of the adenomatous polyposis coli/ß-catenin/T-cell factor 4 (TCF4) pathway and of specificity protein 1 (Sp1)-like factor-dependent transcription. We report that SERCA3 expression increased along the crypts as cells differentiated in normal colonic mucosa and in hyperplastic polyps, was moderately and heterogeneously expressed in colonic adenomas with expression levels inversely correlated with the degree of dysplasia, was barely detectable in well and moderately differentiated adenocarcinomas, and was absent in poorly differentiated tumors. Inhibition of Sp1-like factor-dependent transcription blocked SERCA3 expression during cell differentiation, and SERCA3 expression was induced by the expression of dominant-negative TCF4 in colon cancer cells. These data link SERCA3 expression to the state of differentiation of colonic epithelial cells, and relate SERCA3 expression, already decreased in adenomas, to enhanced adenomatous polyposis coli/ß-catenin/TCF4-dependent signaling and deficient Sp1-like factor-dependent transcription. In conclusion, intracellular calcium homeostasis becomes progressively anomalous during colon carcinogenesis as reflected by deficient SERCA3 expression.






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Copyright © 2005 by the American Society for Investigative Pathology.