This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lockwood, C. J. Articles by Schatz, F. Search for Related Content PubMed PubMed Citation Articles by Lockwood, C. J. Articles by Schatz, F.

(American Journal of Pathology. 2006;168:445-452.)
© 2006 American Society for Investigative Pathology

Regulation of Monocyte Chemoattractant Protein-1 Expression by Tumor Necrosis Factor- and Interleukin-1ß in First Trimester Human Decidual Cells

Implications for Preeclampsia

Charles J. Lockwood^*, Paul Matta^*, Graciela Krikun^*, Louise A. Koopman, Rachel Masch, Paolo Toti, Felice Arcuri, Se-Te Joseph Huang^*, Edmund F. Funai^* and Frederick Schatz^*

From the Department of Obstetrics, Gynecology, and Reproductive Sciences,^* Yale University School of Medicine, New Haven, Connecticut; the Department of Oncology, Biogen Incorporated, Cambridge Massachusetts; the Department of Obstetrics and Gynecology, New York University School of Medicine, New York, New York; and the Department of Human Pathology and Oncology, University of Siena, Siena, Italy

The current study describes a statistically significant increase in macrophages (CD68-positive cells) in the decidua of preeclamptic patients. To elucidate the regulation of this monocyte infiltration, expression of monocyte chemoattractant protein-1 (MCP-1) was assessed in leukocyte-free first trimester decidual cells. Confluent decidual cells were primed for 7 days in either estradiol or estradiol plus medroxyprogesterone acetate to mimic the decidualizing steroidal milieu of the luteal phase and early pregnancy. The medium was exchanged for a serum-free defined medium containing corresponding steroids +/– tumor necrosis factor (TNF)- or interleukin (IL)-1ß. After 24 hours, enzyme-linked immunosorbent assay measurements indicated that the addition of medroxyprogesterone acetate did not affect MCP-1 output, whereas 10 ng/ml of TNF- or IL-1ß increased output by 83.5-fold ± 20.6 and 103.1-fold ± 14.7, respectively (mean ± SEM, n = 8, P < 0.05). Concentration-response comparisons revealed that even 0.01 ng/ml of TNF- or IL-1ß elevated MCP-1 output by more than 15-fold. Western blotting confirmed the enzyme-linked immunosorbent assay results, and quantitative reverse transcriptase-polymerase chain reaction confirmed corresponding effects on MCP-1 mRNA levels. The current study demonstrates that TNF- and IL-1ß enhance MCP-1 in first trimester decidua. This finding suggests a mechanism by which recruitment of excess macrophages to the decidua impairs endovascular trophoblast invasion, the primary placental defect of preeclampsia.

This article has been cited by other articles:

				O. Shynlova, P. Tsui, A. Dorogin, and S. J. Lye Monocyte Chemoattractant Protein-1 (CCL-2) Integrates Mechanical and Endocrine Signals That Mediate Term and Preterm Labor J. Immunol., July 15, 2008; 181(2): 1470 - 1479. [Abstract] [Full Text] [PDF]

				M. Cackovic, C. S. Buhimschi, G. Zhao, E. F. Funai, E. R. Norwitz, E. Kuczynski, C. J. Lockwood, and I. A. Buhimschi Fractional Excretion of Tumor Necrosis Factor-{alpha} in Women With Severe Preeclampsia Obstet. Gynecol., July 1, 2008; 112(1): 93 - 100. [Abstract] [Full Text] [PDF]

				C. J. Lockwood, C. Oner, Y. H. Uz, U. A. Kayisli, S. J. Huang, L. F. Buchwalder, W. Murk, E. F. Funai, and F. Schatz Matrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells Biol Reprod, June 1, 2008; 78(6): 1064 - 1072. [Abstract] [Full Text] [PDF]

				C. J. Lockwood, C.-F. Yen, M. Basar, U. A. Kayisli, M. Martel, I. Buhimschi, C. Buhimschi, S. J. Huang, G. Krikun, and F. Schatz Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells Am. J. Pathol., June 1, 2008; 172(6): 1571 - 1579. [Abstract] [Full Text] [PDF]

				R. H. McIntire, K. G. Ganacias, and J. S. Hunt Programming of Human Monocytes by the Uteroplacental Environment Reproductive Sciences, May 1, 2008; 15(5): 437 - 447. [Abstract] [PDF]

				C. J. LOCKWOOD, G. KRIKUN, R. CAZE, M. RAHMAN, L. F. BUCHWALDER, and F. SCHATZ Decidual Cell-expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia-related Angiogenesis Ann. N.Y. Acad. Sci., April 1, 2008; 1127(1): 67 - 72. [Abstract] [Full Text] [PDF]

				Y.-Y. He, M.-R. Du, P.-F. Guo, X.-J. He, W.-h. Zhou, X.-Y. Zhu, and D.-J. Li Regulation of C-C motif chemokine ligand 2 and its receptor in human decidual stromal cells by pregnancy-associated hormones in early gestation Hum. Reprod., October 1, 2007; 22(10): 2733 - 2742. [Abstract] [Full Text] [PDF]

				S.-K. Kim, A. E. Fouts, and J. C. Boothroyd Toxoplasma gondii Dysregulates IFN-{gamma}-Inducible Gene Expression in Human Fibroblasts: Insights from a Genome-Wide Transcriptional Profiling J. Immunol., April 15, 2007; 178(8): 5154 - 5165. [Abstract] [Full Text] [PDF]

				C. J. Lockwood, P. Toti, F. Arcuri, E. Norwitz, E. F. Funai, S.-T. J. Huang, L. F. Buchwalder, G. Krikun, and F. Schatz Thrombin Regulates Soluble fms-Like Tyrosine Kinase-1 (sFlt-1) Expression in First Trimester Decidua: Implications for Preeclampsia Am. J. Pathol., April 1, 2007; 170(4): 1398 - 1405. [Abstract] [Full Text] [PDF]

				F. Arcuri, L. Buchwalder, P. Toti, M. Cintorino, P. Tosi, C. J. Lockwood, B. Rybalov, and F. Schatz Differential Regulation of Colony Stimulating Factor 1 and Macrophage Migration Inhibitory Factor Expression by Inflammatory Cytokines in Term Human Decidua: Implications for Macrophage Trafficking at the Fetal-Maternal Interface Biol Reprod, March 1, 2007; 76(3): 433 - 439. [Abstract] [Full Text] [PDF]