help button home button Am J Pathol Epitomics, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murthi, P.
Right arrow Articles by Kalionis, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murthi, P.
Right arrow Articles by Kalionis, B.
(American Journal of Pathology. 2006;168:511-518.)
© 2006 American Society for Investigative Pathology

Homeobox Gene HLX1Expression Is Decreased in Idiopathic Human Fetal Growth Restriction

Padma Murthi*{dagger}, Vicki Doherty*, Joanne Said*{dagger}, Susan Donath{ddagger}, Shaun P. Brennecke*{dagger} and Bill Kalionis*{dagger}

From the Department of Perinatal Medicine,* Pregnancy Research Centre, The Royal Women’s Hospital, Carlton, Victoria; the Department of Obstetrics and Gynaecology,{dagger} University of Melbourne, The Royal Women’s Hospital Campus, Carlton, Victoria; and the Clinical Epidemiology and Biostatistics Unit,{ddagger} Royal Children’s Hospital, Parkville, Victoria, Australia

Fetal growth restriction (FGR) is a clinically significant pregnancy disorder in which the fetus fails to achieve its full growth potential in utero. Identifiable causes of FGR account for ~30% of cases, but the remainder are idiopathic and are frequently associated with placental malfunction. Previously, we isolated the homeobox gene HLX1 and provided evidence for a regulatory role in normal placental development. Here, we investigated whether placental HLX1 expression levels are changed in placentas from idiopathic FGR pregnancies. Real-time polymerase chain reaction quantitation showed reduced HLX1 mRNA levels with advancing gestation age (preterm control placentas, 27 to 35 weeks, 1.1 ± 0.3, n = 13, versus term placentas 36 to 41 weeks, 0.74 ± 0.02, n = 12, P < 0.005). FGR-affected placentas had significantly lower levels of HLX1 expression compared with gestation age-matched controls (0.36 ± 0.07 versus 1.05 ± 0.2, n = 25, P < 0.001). Immunoblotting with a rabbit polyclonal HLX1 antibody revealed reduced levels of HLX1 in FGR-affected placentas compared with controls (481.07 ± 12.3 versus 2766.7 ± 30.3, n = 10, P < 0.001). Immunohistochemistry showed a qualitative decrease in HLX1 immunoreactivity in FGR-affected term placentas compared with controls. This is the first demonstration that a homeobox transcriptional regulator shows altered expression in an important human placental disorder, suggesting that decreased HLX1 levels contribute to the abnormalities in placental developmental seen in idiopathic FGR.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
G. Rajaraman, P. Murthi, N. Pathirage, S. P. Brennecke, and B. Kalionis
Downstream Targets of Homeobox Gene HLX Show Altered Expression in Human Idiopathic Fetal Growth Restriction
Am. J. Pathol., January 1, 2010; 176(1): 278 - 287.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
P. Murthi, V. L. Doherty, J. M. Said, S. Donath, S. P. Brennecke, and B. Kalionis
Homeobox gene ESX1L expression is decreased in human pre-term idiopathic fetal growth restriction
Mol. Hum. Reprod., May 1, 2006; 12(5): 335 - 340.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Society for Investigative Pathology.