help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okada, H.
Right arrow Articles by Suzuki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okada, H.
Right arrow Articles by Suzuki, H.
(American Journal of Pathology. 2006;168:737-747.)
© 2006 American Society for Investigative Pathology

Dexamethasone Induces Connective Tissue Growth Factor Expression in Renal Tubular Epithelial Cells in a Mouse Strain-Specific Manner

Hirokazu Okada*, Tomohiro Kikuta*, Tsutomu Inoue*, Yoshihiko Kanno*, Shinichi Ban{dagger}, Takeshi Sugaya{ddagger}, Masaharu Takigawa§ and Hiromichi Suzuki*

From the Departments of Nephrology* and Pathology,{dagger} Saitama Medical School, Saitama; the Center of Tsukuba Advanced Research Alliance,{ddagger} Institute of Applied Biochemistry, University of Tsukuba, Ibaraki; and the Department of Biochemistry and Molecular Dentistry,§ Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan

Connective tissue growth factor (CTGF), a downstream mediator of transforming growth factor-ß1, mediates mesangial cell/fibroblast proliferation and extracellular matrix production by renal cells. Here, we show that renal tubular epithelial cells from patients with minimal change nephritic syndrome produced CTGF after glucocorticoid treatment. In addition, the glucocorticoid dexamethasone (DEX) increased CTGF mRNA levels in the kidneys of C57B6 but not SJL mice and produced intermediate CTGF mRNA levels in the kidneys of F1 (C57B6 x SJL) mice, midway between the levels found for parental strains. DEX also increased CTGF mRNA levels in cultured tubular epithelial cells derived from C57B6 (mProx24) but not SJL (MCT) mice via transcriptional up-regulation of CTGF mRNA. Transient transfection experiments using luciferase reporter constructs bearing CTGF promoter fragments revealed that the –897- to –628-bp fragment contained DEX-responsive positive regulatory elements, which were active in mProx24 but not MCT cells. Long-term DEX treatment resulted in fibronectin deposition in the kidneys of C57B6 but not SJL mice, and this effect was inhibited by co-administration of CTGF anti-sense oligodeoxynucleotides. Thus, glucocorticoid-induced renal fibrogenesis seems to be influenced by genetic background, with the critical DEX-responsive elements in the –897- to –628-bp region of the CTGF promoter.




This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
H. Okada, T. Inoue, T. Kikuta, N. Kato, Y. Kanno, N. Hirosawa, Y. Sakamoto, T. Sugaya, and H. Suzuki
Poly(ADP-Ribose) Polymerase-1 Enhances Transcription of the Profibrotic CCN2 Gene
J. Am. Soc. Nephrol., May 1, 2008; 19(5): 933 - 942.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
S. Gauer, V. Segitz, and M. Goppelt-Struebe
Aldosterone induces CTGF in mesangial cells by activation of the glucocorticoid receptor
Nephrol. Dial. Transplant., November 1, 2007; 22(11): 3154 - 3159.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. Yamamoto, E. Noiri, Y. Ono, K. Doi, K. Negishi, A. Kamijo, K. Kimura, T. Fujita, T. Kinukawa, H. Taniguchi, et al.
Renal L-Type Fatty Acid Binding Protein in Acute Ischemic Injury
J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2894 - 2902.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Society for Investigative Pathology.