This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kramerov, A. A. Articles by Ljubimov, A. V. Search for Related Content PubMed PubMed Citation Articles by Kramerov, A. A. Articles by Ljubimov, A. V.

(American Journal of Pathology. 2006;168:1722-1736.)
© 2006 American Society for Investigative Pathology

Expression of Protein Kinase CK2 in Astroglial Cells of Normal and Neovascularized Retina

Andrei A. Kramerov^*, Mehrnoosh Saghizadeh^*, Hao Pan, Andrea Kabosova^*, Mathias Montenarh, Khalil Ahmed, John S. Penn^¶, Candy K. Chan^||, David R. Hinton^||, Maria B. Grant and Alexander V. Ljubimov^*

From the Ophthalmology Research Laboratories,^* Cedars-Sinai Medical Center, University of California at Los Angeles School of Medicine, Los Angeles, California; the Department of Pharmacology, University of Florida, Gainesville, Florida; the Minneapolis Veterans Affairs Medical Center, University of Minnesota, Minneapolis, Minnesota; the Department of Ophthalmology and Visual Sciences,^¶ Vanderbilt University School of Medicine, Nashville, Tennessee; the Departments of Pathology and Ophthalmology,|| University of Southern California, Los Angeles, California; and Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Homburg, Germany

We previously documented protein kinase CK2 involvement in retinal neovascularization. Here we describe retinal CK2 expression and combined effects of CK2 inhibitors with the somatostatin analog octreotide in a mouse model of oxygen-induced retinopathy (OIR). CK2 expression in human and rodent retinas with and without retinopathy and in astrocytic and endothelial cultures was examined by immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction. A combination of CK2 inhibitors, emodin or 4,5,6,7-tetrabromobenzotriazole, with octreotide was injected intraperitoneally from postnatal (P) day P11 to P17 to block mouse OIR. All CK2 subunits (, ', ß) were expressed in retina, and a novel CK2 splice variant was detected by reverse transcriptase-polymerase chain reaction. CK2 antibodies primarily reacted with retinal astrocytes, and staining was increased around new intraretinal vessels in mouse OIR and rat retinopathy of prematurity, whereas preretinal vessels were negative. Cultured astrocytes showed increased perinuclear CK2 staining compared to endothelial cells. In the OIR model, CK2 mRNA expression increased modestly on P13 but not on P17. Octreotide combined with emodin or 4,5,6,7-tetrabromobenzotriazole blocked mouse retinal neovascularization more efficiently than either compound alone. Based on its retinal localization, CK2 may be considered a new immunohistochemical astrocytic marker, and combination of CK2 inhibitors and octreotide may be a promising future treatment for proliferative retinopathies.

This article has been cited by other articles:

				I. A. Bhutto, K. Uno, C. Merges, L. Zhang, D. S. McLeod, and G. A. Lutty Reduction of Endogenous Angiogenesis Inhibitors in Bruch's Membrane of the Submacular Region in Eyes With Age-Related Macular Degeneration Arch Ophthalmol, May 1, 2008; 126(5): 670 - 678. [Abstract] [Full Text] [PDF]

				A. Kabosova, D. T. Azar, G. A. Bannikov, K. P. Campbell, M. Durbeej, R. F. Ghohestani, J. C. R. Jones, M. C. Kenney, M. Koch, Y. Ninomiya, et al. Compositional Differences between Infant and Adult Human Corneal Basement Membranes Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 4989 - 4999. [Abstract] [Full Text] [PDF]