| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Department of Surgery,* Center for Surgical Research, University of Alabama at Birmingham, Birmingham, Alabama; the Department of Orthopedic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania; and the Department of Pathology, University of Michigan Medical Center, Ann Arbor, Michigan
Posttraumatic activation of macrophages enhances development of systemic inflammation/immunosuppression and organ dysfunction. We hypothesized that Kupffer cells are the main source of monocyte chemoattractant protein-1 (MCP-1) production after trauma-hemorrhage, that administration of 17ß-estradiol (E2) after trauma-hemorrhage modulates MCP-1 release and reduces remote organ damage, and that salutary effects of E2 are mediated via estrogen receptor (ER)-
. To test these hypotheses, female B57BL/J6 mice received E2 (50 µg/25 g) or vehicle after trauma-hemorrhage and female 129 Sve ER-ß–/– transgenic mice and ovariectomized wild-type mice received E2 or ER- agonist propyl pyrazole triol (50 µg/25 g) after trauma-hemorrhage. Systemic MCP-1 and interleukin-6 and their release by liver, spleen, and lung macrophages were determined by flow cytometry 4 hours after trauma-hemorrhage. Prior Kupffer cell depletion with gadolinium chloride significantly decreased systemic MCP-1 and interleukin-6 after trauma-hemorrhage and was associated with decreased edema/neutrophil infiltration in lung and liver. Kupffer cells were the only macrophages showing significant MCP-1 release, which was markedly reduced by E2 or propyl pyrazole triol in wild-type and in ER-ß–/– mice. Pretreatment of mice with anti-MCP-1 antiserum prevented an increase in myeloperoxidase and edema in lung and liver. These findings suggest that Kupffer cell-derived MCP-1 plays a major role in remote organ dysfunction after trauma-hemorrhage.
This article has been cited by other articles:
 |
|
 |
|
  J. P. Luyendyk, B. P. Sullivan, G. L. Guo, and R. Wang Tissue Factor-Deficiency and Protease Activated Receptor-1-Deficiency Reduce Inflammation Elicited by Diet-Induced Steatohepatitis in Mice Am. J. Pathol., January 1, 2010; 176(1): 177 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Hsieh, J.-T. Hsu, Y.-C. Hsieh, M. Frink, R. Raju, W. J. Hubbard, K. I. Bland, and I. H. Chaudry Suppression of Activation and Costimulatory Signaling in Splenic CD4+ T Cells after Trauma-Hemorrhage Reduces T-Cell Function: A Mechanism of Post-Traumatic Immune Suppression Am. J. Pathol., October 1, 2009; 175(4): 1504 - 1514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Hsieh, E. A. Nickel, J. Chen, M. G. Schwacha, M. A. Choudhry, K. I. Bland, and I. H. Chaudry Mechanism of the Salutary Effects of Estrogen on Kupffer Cell Phagocytic Capacity following Trauma-Hemorrhage: Pivotal Role of Akt Activation J. Immunol., April 1, 2009; 182(7): 4406 - 4414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Flohe, S. Flohe, and F. U. Schade Invited review: Deterioration of the immune system after trauma: signals and cellular mechanisms Innate Immunity, December 1, 2008; 14(6): 333 - 344. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Kitamura, M. Ito, T. Yuasa, C. Kikuguchi, A. Hijikata, M. Takayama, Y. Kimura, R. Yokoyama, T. Kaji, and O. Ohara Genome-wide identification and characterization of transcripts translationally regulated by bacterial lipopolysaccharide in macrophage-like J774.1 cells Physiol Genomics, October 8, 2008; 33(1): 121 - 132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Hsieh, M. Frink, Y.-C. Hsieh, W.-H. Kan, J.-T. Hsu, M. G. Schwacha, M. A. Choudhry, and I. H. Chaudry The Role of MIP-1{alpha} in the Development of Systemic Inflammatory Response and Organ Injury following Trauma Hemorrhage J. Immunol., August 15, 2008; 181(4): 2806 - 2812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-H. Kan, C.-H. Hsieh, M. G. Schwacha, M. A. Choudhry, R. Raju, K. I. Bland, and I. H. Chaudry Flutamide protects against trauma-hemorrhage-induced liver injury via attenuation of the inflammatory response, oxidative stress, and apopotosis J Appl Physiol, August 1, 2008; 105(2): 595 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Hod, C. M. Cadwell, J. S. Liepkalns, J. C. Zimring, S. A. Sokol, D. A. Schirmer, J. Jhang, and S. L. Spitalnik Cytokine storm in a mouse model of IgG-mediated hemolytic transfusion reactions Blood, August 1, 2008; 112(3): 891 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Raju and I. H. Chaudry Sex Steroids/Receptor Antagonist: Their Use as Adjuncts After Trauma-Hemorrhage for Improving Immune/Cardiovascular Responses and for Decreasing Mortality from Subsequent Sepsis Anesth. Analg., July 1, 2008; 107(1): 159 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Choudhry and I. H. Chaudry 17{beta}-Estradiol: a novel hormone for improving immune and cardiovascular responses following trauma-hemorrhage J. Leukoc. Biol., March 1, 2008; 83(3): 518 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Hsieh, M. Frink, C.-H. Hsieh, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry Downregulation of migration inhibitory factor is critical for estrogen-mediated attenuation of lung tissue damage following trauma-hemorrhage Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1227 - L1232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Meldrum G-Protein-Coupled Receptor 30 Mediates Estrogen's Nongenomic Effects after Hemorrhagic Shock and Trauma Am. J. Pathol., April 1, 2007; 170(4): 1148 - 1151. [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Hsieh, H.-P. Yu, M. Frink, T. Suzuki, M. A. Choudhry, M. G. Schwacha, and I. H. Chaudry G Protein-Coupled Receptor 30-Dependent Protein Kinase A Pathway Is Critical in Nongenomic Effects of Estrogen in Attenuating Liver Injury after Trauma-Hemorrhage Am. J. Pathol., April 1, 2007; 170(4): 1210 - 1218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Frink, A. Lu, B. M. Thobe, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, S. L. Kunkel, and I. H. Chaudry Monocyte chemoattractant protein-1 influences trauma-hemorrhage-induced distal organ damage via regulation of keratinocyte-derived chemokine production Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1110 - R1116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Frink, B. M. Thobe, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry 17beta-Estradiol inhibits keratinocyte-derived chemokine production following trauma-hemorrhage Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L585 - L591. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
