help button home button Am J Pathol Epitomics, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chiosea, S.
Right arrow Articles by Dhir, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chiosea, S.
Right arrow Articles by Dhir, R.
(American Journal of Pathology. 2006;169:1812-1820.)
© 2006 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2006.060480

Up-Regulation of Dicer, a Component of the MicroRNA Machinery, in Prostate Adenocarcinoma

Simion Chiosea*, Elena Jelezcova{dagger}{ddagger}, Uma Chandran*, Marie Acquafondata*, Teresa McHale*, Robert W. Sobol{dagger}{ddagger} and Rajiv Dhir*

From the Department of Pathology,* University of Pittsburgh Medical Center, Pittsburgh; Department of Pharmacology,{dagger} University of Pittsburgh School of Medicine; and University of Pittsburgh Cancer Institute,{ddagger} Hillman Cancer Center, Pittsburgh, Pennsylvania

MicroRNAs are small noncoding 18- to 24-nt RNAs that are predicted to regulate expression of as many as 30% of protein-encoding genes. In prostate adenocarcinoma, 39 microRNAs are up-regulated, and six microRNAs are down-regulated. Production and function of microRNA requires coordinated processing by proteins of the microRNA machinery. Dicer, an RNase III endonuclease, is an essential component of the microRNA machinery. From a gene array analysis of 16 normal prostate tissue samples, 64 organ-confined, and four metastatic prostate adenocarcinomas, we identified an up-regulation of major components of the microRNA machinery, including Dicer, in metastatic prostate adenocarcinoma. Immunohistochemical studies on a tissue microarray consisting of 232 prostate specimens confirmed up-regulation of Dicer in prostatic intraepithelial neoplasia and in 81% of prostate adenocarcinoma. The increased Dicer level in prostate adenocarcinoma correlated with clinical stage, lymph node status, and Gleason score. Western blot analysis of benign and neoplastic prostate cell lines further confirmed Dicer up-regulation in prostate adenocarcinoma. Dicer up-regulation may explain an almost global increase of microRNA expression in prostate adenocarcinoma. The presence of up-regulated microRNA machinery may predict the susceptibility of prostate adenocarcinoma to RNA interference-based therapy.





This article has been cited by other articles:


Home page
RNAHome page
W.-X. Wang, B. R. Wilfred, Y. Hu, A. J. Stromberg, and P. T. Nelson
Anti-Argonaute RIP-Chip shows that miRNA transfections alter global patterns of mRNA recruitment to microribonucleoprotein complexes
RNA, February 1, 2010; 16(2): 394 - 404.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
J. W.F. Catto, S. Miah, H. C. Owen, H. Bryant, K. Myers, E. Dudziec, S. Larre, M. Milo, I. Rehman, D. J. Rosario, et al.
Distinct MicroRNA Alterations Characterize High- and Low-Grade Bladder Cancer
Cancer Res., November 1, 2009; 69(21): 8472 - 8481.
[Abstract] [Full Text] [PDF]


Home page
RNAHome page
G. Sotiropoulou, G. Pampalakis, E. Lianidou, and Z. Mourelatos
Emerging roles of microRNAs as molecular switches in the integrated circuit of the cancer cell
RNA, August 1, 2009; 15(8): 1443 - 1461.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. Ueda, G. Kohanbash, K. Sasaki, M. Fujita, X. Zhu, E. R. Kastenhuber, H. A. McDonald, D. M. Potter, R. L. Hamilton, M. T. Lotze, et al.
Dicer-regulated microRNAs 222 and 339 promote resistance of cancer cells to cytotoxic T-lymphocytes by down-regulation of ICAM-1
PNAS, June 30, 2009; 106(26): 10746 - 10751.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
H. Kuruma, Y. Kamata, H. Takahashi, K. Igarashi, T. Kimura, K. Miki, J. Miki, H. Sasaki, N. Hayashi, and S. Egawa
Staphylococcal Nuclease Domain-Containing Protein 1 as a Potential Tissue Marker for Prostate Cancer
Am. J. Pathol., June 1, 2009; 174(6): 2044 - 2050.
[Abstract] [Full Text] [PDF]


Home page
NEJMHome page
W. M. Merritt, Y. G. Lin, L. Y. Han, A. A. Kamat, W. A. Spannuth, R. Schmandt, D. Urbauer, L. A. Pennacchio, J.-F. Cheng, A. M. Nick, et al.
Dicer, Drosha, and Outcomes in Patients with Ovarian Cancer
N. Engl. J. Med., December 18, 2008; 359(25): 2641 - 2650.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
Y. Horikawa, C. G. Wood, H. Yang, H. Zhao, Y. Ye, J. Gu, J. Lin, T. Habuchi, and X. Wu
Single Nucleotide Polymorphisms of microRNA Machinery Genes Modify the Risk of Renal Cell Carcinoma
Clin. Cancer Res., December 1, 2008; 14(23): 7956 - 7962.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
S. Asada, T. Takahashi, K. Isodono, A. Adachi, H. Imoto, T. Ogata, T. Ueyama, H. Matsubara, and H. Oh
Downregulation of Dicer expression by serum withdrawal sensitizes human endothelial cells to apoptosis
Am J Physiol Heart Circ Physiol, December 1, 2008; 295(6): H2512 - H2521.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
Y. Liu, S. A. Perdreau, P. Chatterjee, L. Wang, S.-F. Kuan, and A. Duensing
Imatinib Mesylate Induces Quiescence in Gastrointestinal Stromal Tumor Cells through the CDH1-SKP2-p27Kip1 Signaling Axis
Cancer Res., November 1, 2008; 68(21): 9015 - 9023.
[Abstract] [Full Text] [PDF]


Home page
J. Am. Soc. Nephrol.Home page
J.J. D. Ho and P. A. Marsden
Dicer Cuts the Kidney
J. Am. Soc. Nephrol., November 1, 2008; 19(11): 2043 - 2046.
[Full Text] [PDF]


Home page
Cancer Res.Home page
S. Ambs, R. L. Prueitt, M. Yi, R. S. Hudson, T. M. Howe, F. Petrocca, T. A. Wallace, C.-G. Liu, S. Volinia, G. A. Calin, et al.
Genomic Profiling of MicroRNA and Messenger RNA Reveals Deregulated MicroRNA Expression in Prostate Cancer
Cancer Res., August 1, 2008; 68(15): 6162 - 6170.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
L. Zhang, S. Volinia, T. Bonome, G. A. Calin, J. Greshock, N. Yang, C.-G. Liu, A. Giannakakis, P. Alexiou, K. Hasegawa, et al.
Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer
PNAS, May 13, 2008; 105(19): 7004 - 7009.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
K. P. Porkka, M. J. Pfeiffer, K. K. Waltering, R. L. Vessella, T. L.J. Tammela, and T. Visakorpi
MicroRNA Expression Profiling in Prostate Cancer
Cancer Res., July 1, 2007; 67(13): 6130 - 6135.
[Abstract] [Full Text] [PDF]


Home page
Hum Mol GenetHome page
C. Blenkiron and E. A. Miska
miRNAs in cancer: approaches, aetiology, diagnostics and therapy
Hum. Mol. Genet., April 15, 2007; 16(R1): R106 - R113.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
S. Chiosea, E. Jelezcova, U. Chandran, J. Luo, G. Mantha, R. W. Sobol, and S. Dacic
Overexpression of Dicer in Precursor Lesions of Lung Adenocarcinoma
Cancer Res., March 1, 2007; 67(5): 2345 - 2350.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Society for Investigative Pathology.