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(American Journal of Pathology. 2006;169:2148-2160.)
© 2006 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2006.060570

Simultaneous Dystrophin and Dysferlin Deficiencies Associated with High-Level Expression of the Coxsackie and Adenovirus Receptor in Transgenic Mice

Christian A. Shaw*, Nancy Larochelle*{dagger}, Roy W.R. Dudley{ddagger}, Hanns Lochmuller§, Gawiyou Danialou{ddagger}, Basil J. Petrof{ddagger}, George Karpati*, Paul C. Holland* and Josephine Nalbantoglu*

From the Montreal Neurological Institute and Department of Neurology and Neurosurgery* and the Respiratory Division,{ddagger} McGill University Health Center and Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada; Genzentrum,{dagger} Munich, Germany; and the Department of Neurology and Friedrich-Baur-Institute,§ Ludwig-Maximilians-University, Munich, Germany

The Coxsackie and adenovirus receptor (CAR), a cell adhesion molecule of the immunoglobulin superfamily, is usually confined to the sarcolemma at the neuromuscular junction in mature skeletal muscle fibers. Previously, we reported that adenovirus-mediated gene transfer is greatly facilitated in hemizygous transgenic mice with extrasynaptic CAR expression driven by a muscle-specific promoter. However, in the present study, when these mice were bred to homozygosity, they developed a severe myopathic phenotype and died prematurely. Large numbers of necrotic and regenerating fibers were present in the skeletal muscle of the homozygous CAR transgenics. The myopathy was further characterized by increased levels of caveolin-3 and ß-dystroglycan and decreased levels of dystrophin, dysferlin, and neuronal nitric-oxide synthase. Even the hemizygotes manifested a subtle phenotype, displaying deficits in isometric force generation and perturbed mitogen-activated protein kinase (MAPK—erk1/2) activation during contraction. There are few naturally occurring or engineered mouse lines showing as severe a skeletal myopathy as observed with ectopic expression of CAR in the homozygotes. Taken together, these findings suggest that substantial overexpression of CAR may lead to physiological dysfunction by disturbing sarcolemmal integrity (through dystrophin deficiency), impairing sarcolemmal repair (through dysferlin deficiency), and interfering with normal signaling (through alterations in caveolin-3 and neuronal nitric-oxide synthase levels).




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Coxsackievirus and Adenovirus Receptor Is Up-Regulated in Migratory Germ Cells during Passage of the Blood-Testis Barrier
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