help button home button Am J Pathol Angiogenesis Meeting
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petty, A. P.
Right arrow Articles by Lindsey, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petty, A. P.
Right arrow Articles by Lindsey, J. S.
(American Journal of Pathology. 2007;170:1763-1780.)
© 2007 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2007.060969

Overexpression of Carcinoma and Embryonic Cytotrophoblast Cell-Specific Mig-7 Induces Invasion and Vessel-Like Structure Formation

Aaron P. Petty*, Kiera L. Garman{dagger}, Virginia D. Winn{ddagger}, Celee M. Spidel§ and J. Suzanne Lindsey*{dagger}¶||

From the School of Molecular Biosciences,* the Department of Pharmaceutical Sciences,{dagger} College of Pharmacy, the Center for Reproductive Biology, and the Cancer Prevention and Research Center,|| Washington State University, Pullman, Washington; the Department of Obstetrics and Gynecology,{ddagger} Sections of Maternal-Fetal Medicine and Reproductive Sciences, University of Colorado Health Sciences Center, Denver, Colorado; and the Department of Pharmaceutical Sciences,§ the School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, Texas

Molecular requirements for carcinoma cell interactions with the microenvironment are critical for disease progression but are poorly understood. Integrin {alpha}vß5, which senses the extracellular matrix, is important for carcinoma cell dissemination in vivo. {alpha}vß5 signaling induces Mig-7, a novel human gene product that is apparently carcinoma-specific. We hypothesized that Mig-7 expression facilitates tumor cell dissemination by increasing invasion and vasculogenic mimicry. Results show that embryonic cytotrophoblasts up-regulated Mig-7 expression before they acquired an invasive phenotype capable of pseudovasculogenesis. Mig-7 protein primarily co-localized with vasculogenic mimicry markers factor VIII-associated antigen, vascular endothelial-cadherin, and laminin 5 {gamma}2 chain domain III fragment in lymph node metastases. Overexpression of Mig-7 increased {gamma}2 chain domain III fragments known to contain epidermal growth factor (EGF)-like repeats that can activate EGF receptor. Interestingly, EGF also induced Mig-7 expression. Carcinoma cell adhesion to laminins was significantly reduced by Mig-7 expression. Remarkably, in two-dimensional and three-dimensional Matrigel cultures, Mig-7 expression caused invasion and vessel-like structures. Melanoma cells, which were previously characterized to invade aggressively and to undergo vasculogenic mimicry, expressed Mig-7. Taken together, these data suggest that Mig-7 expression allows cells to sense their environment, to invade, and to form vessel-like structures through a novel relationship with laminin 5 {gamma}2 chain domain III fragments.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
G. P. Robertson
Mig-7 Linked to Vasculogenic Mimicry
Am. J. Pathol., May 1, 2007; 170(5): 1454 - 1456.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the American Society for Investigative Pathology.