Published online before print April 19, 2007

This Article Full Text Full Text (PDF) Supplemental Material All Versions of this Article: ajpath.2007.060881v1 170/6/2019    most recent Purchase Article View Shopping Cart Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tamagawa-Mineoka, R. Articles by Kishimoto, S. Search for Related Content PubMed Articles by Tamagawa-Mineoka, R. Articles by Kishimoto, S.

(American Journal of Pathology. 2007;170:2019-2029.)
© 2007 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2007.060881

The Role of Platelets in Leukocyte Recruitment in Chronic Contact Hypersensitivity Induced by Repeated Elicitation

Risa Tamagawa-Mineoka^*, Norito Katoh^*, Eiichiro Ueda^*, Hideya Takenaka^*, Masakazu Kita and Saburo Kishimoto^*

From the Departments of Dermatology^* and Microbiology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, Japan

Platelets have been shown to be important in inflammation, but their role in chronic allergic dermatitis remains unclear. To investigate the role of platelets in a mouse model of chronic contact hypersensitivity induced by repeated elicitation, mice were sensitized and repeatedly elicited in ears with hapten, with or without platelet depletion, by administering antiplatelet antibody or busulfan. Ear thickness, leukocyte infiltration, serum IgE, and scratching behavior significantly decreased in thrombocytopenic mice. cDNA microarray of ear tissue showed reduced gene expression associated with Th2 lymphocytes. Flow cytometry showed increased P-selectin expression on platelets and an increased number of platelet-leukocyte aggregates in blood of repeatedly elicited mice, compared with sham-sensitized mice. In thrombocytopenic mice, inflammation was restored by platelet infusion, which was blocked by platelets from P-selectin-deficient mice or by pretreating platelets with anti-P-selectin antibody. Moreover, injection of activated platelet supernatant into ears led to increased leukocyte infiltration, which was blocked by pretreating platelets with antiplatelet compounds or neutralizing several chemokines in the platelet supernatant. These results suggest that platelets induce leukocyte recruitment into skin by forming platelet-leukocyte aggregates via P-selectin in blood and secreting chemokines at inflamed sites. Therefore, controlling platelet activity may be useful for treatment of chronic allergic dermatitis.

This article has been cited by other articles:

				R. J. Ludwig, P. Bergmann, J. Garbaraviciene, E. v. Stebut, H. H. Radeke, J. Gille, S. Diehl, K. Hardt, R. Henschler, R. Kaufmann, et al. Platelet, Not Endothelial, P-Selectin Expression Contributes to Generation of Immunity in Cutaneous Contact Hypersensitivity Am. J. Pathol., March 1, 2010; 176(3): 1339 - 1345. [Abstract] [Full Text] [PDF]

				T. Hara, K. Shimizu, F. Ogawa, K. Yanaba, Y. Iwata, E. Muroi, M. Takenaka, K. Komura, M. Hasegawa, M. Fujimoto, et al. Platelets Control Leukocyte Recruitment in a Murine Model of Cutaneous Arthus Reaction Am. J. Pathol., January 1, 2010; 176(1): 259 - 269. [Abstract] [Full Text] [PDF]

				H. Zhou, G. Andonegui, C. H. Y. Wong, and P. Kubes Role of Endothelial TLR4 for Neutrophil Recruitment into Central Nervous System Microvessels in Systemic Inflammation J. Immunol., October 15, 2009; 183(8): 5244 - 5250. [Abstract] [Full Text] [PDF]

				B. Ho-Tin-Noe, C. Carbo, M. Demers, S. M. Cifuni, T. Goerge, and D. D. Wagner Innate Immune Cells Induce Hemorrhage in Tumors during Thrombocytopenia Am. J. Pathol., October 1, 2009; 175(4): 1699 - 1708. [Abstract] [Full Text] [PDF]