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Originally published online as doi:10.2353/ajpath.2007.070142 on September 20, 2007

Published online before print September 20, 2007
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(American Journal of Pathology. 2007;171:1682-1690.)
© 2007 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2007.070142

Cartilage Oligometric Matrix Protein-Angiopoietin-1 Promotes Revascularization Through Increased Survivin Expression in Dermal Endothelial Cells of Skin Grafts in Mice

Seung-Jae Byun*{dagger}, Kyu-Sil Choi*, Sung Hoon Park*, Nam Woo Cho{ddagger}, Chang Hyun Yoo§, Ki Jung Yun, Young Jun Koh||, Gou Young Koh||, Byung Jun So{dagger} and Kwon-Ha Yoon*

From the Institute for Radiological Imaging Science* and the Departments of Pathology and Vascular and Transplantation Surgery,{dagger} Wonkwang University School of Medicine, Jeonbuk, Korea; the Department of Vascular Surgery,§ Good Gang-An Hospital, Pusan, Korea; the Biomedical Research Center and Department of Biological Sciences,|| Korea Advanced Institute of Science and Technology, Daejeon, Korea; and the Department of Molecular and Cellular Biology,{ddagger} Harvard University, Cambridge, Massachusetts

The present study examined the effects of cartilage oligometric matrix protein angiopoietin-1 (COMP-Ang1) on the revascularization of mice skin grafts. Full-thickness skin grafts were autotransferred into BALB/c mice. The donor grafts were soaked in COMP-Ang1 protein (50 µg/ml, n = 10) or in bovine serum albumin (BSA) (50 µg/ml, n = 10) dissolved in 1 ml of sterile, phosphate-buffered saline for 5 minutes before transfer. Revascularization of the grafts was monitored using an intravital microscope on postoperative days 3, 4, and 5. Morphological and immunohistochemical analyses were performed to evaluate platelet-endothelial cell adhesion molecule-1 and survivin expression and apoptotic signal in the transplanted grafts. Grafts soaked in COMP-Ang1 (COMP-Ang1 group) showed significantly increased revascularization compared with grafts soaked in BSA (BSA group) on intravital microscopy and platelet-endothelial cell adhesion molecule-1 staining. The COMP-Ang1 group showed a significant increase of survivin expression in the endothelial cells and a reduction of apoptotic signal in comparison to the BSA group. Therefore, we believe that COMP-Ang1 provides the therapeutic benefit of enhancing the survival of vascular endothelial cells during transplantation of skin graft.






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Copyright © 2007 by the American Society for Investigative Pathology.