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Originally published online as doi:10.2353/ajpath.2008.070793 on April 1, 2008

Published online before print April 1, 2008
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(American Journal of Pathology. 2008;172:1238-1247.)
© 2008 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2008.070793

Deletion of the Met Tyrosine Kinase in Liver Progenitor Oval Cells Increases Sensitivity to Apoptosis in Vitro

Gaelle del Castillo*, Valentina M. Factor{dagger}, Margarita Fernández*, Alberto Álvarez-Barrientos{ddagger}, Isabel Fabregat§, Snorri S. Thorgeirsson{dagger} and Aránzazu Sánchez*

From the Department Bioquímica y Biología Molecular II,* Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain; Laboratory of Experimental Carcinogenesis,{dagger} Center for Cancer Research, National Cancer Institute/National Institutes of Health, Bethesda, Maryland; Unidad de Citometría,{ddagger} Fundación Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain; Fundació Institut d’Investigació Biomèdica de Bellvitge,§ Centre d'Oncologia Molecular, L'Hospitalet, Barcelona, Spain

The hepatocyte growth factor (HGF)/Met signaling system is essential for liver development, homeostasis, and function. In this study, we took advantage of a liver-specific, Met-conditional knockout mouse generated in our laboratory to address the molecular mechanisms of HGF/Met signaling in adult liver progenitor cell (oval cell) biology. For this purpose, we isolated oval cells from 3,5-diethoxycarbonyl-1,4-dihydro-collidine-treated Metflx/flx mice and established oval cell-derived cell lines that carried either functional (Metflx/flx) or a nonfunctional (Met–/–) met gene using virus-mediated Cre-loxP recombination. Oval cells lacking Met tyrosine kinase activity displayed neither Met phosphorylation nor activation of downstream targets and were refractory to HGF stimulation. Although Met–/– and Metflx/flx cells proliferated at similar rates under 10% serum, Met-deficient cells demonstrated decreased cell viability and were more prone to apoptosis when challenged with either serum starvation or the pro-apoptotic cytokine transforming growth factor-β. Treatment with HGF reduced transforming growth factor-β-mediated cell death in Metflx/flx but not Met–/– cells. Importantly, Metflx/flx and Met–/– cells both constitutively expressed hgf, and conditioned medium from serum-starved oval cells exhibited anti-apoptotic activity in Metflx/flx cells. Furthermore, serum-starved Metflx/flx cells showed persistent activation of the Met tyrosine kinase, suggesting HGF/Met autocrine regulation. In conclusion, these data reveal a critical, functional role for Met in oval cell survival through an autocrine mechanism.






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