help button home button Am J Pathol ASIP WHAT IS IT?
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published online as doi:10.2353/ajpath.2008.080234 on October 30, 2008

Published online before print October 30, 2008
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
ajpath.2008.080234v1
173/6/1669    most recent
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Hochmeister, S.
Right arrow Articles by Bradl, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hochmeister, S.
Right arrow Articles by Bradl, M.
(American Journal of Pathology. 2008;173:1669-1681.)
© 2008 American Society for Investigative Pathology
DOI: 10.2353/ajpath.2008.080234

After Injection into the Striatum, in Vitro-Differentiated Microglia- and Bone Marrow-Derived Dendritic Cells Can Leave the Central Nervous System via the Blood Stream

Sonja Hochmeister*, Manuel Zeitelhofer*, Jan Bauer*, Eva-Maria Nicolussi*, Marie-Therese Fischer*, Bernhard Heinke{dagger}, Edgar Selzer{ddagger}, Hans Lassmann* and Monika Bradl*

From the Department of Neuroimmunology,* and the Department of Neurophysiology,{dagger} Center for Brain Research, Medical University Vienna, Vienna; and the Department of Radiotherapy and Radiobiology,{ddagger} General Hospital Vienna, Vienna, Austria

The prototypic migratory trail of tissue-resident dendritic cells (DCs) is via lymphatic drainage. Since the central nervous system (CNS) lacks classical lymphatic vessels, and antigens and cells injected into both the CNS and cerebrospinal fluid have been found in deep cervical lymph nodes, it was thought that CNS-derived DCs exclusively used the cerebrospinal fluid pathway to exit from tissues. It has become evident, however, that DCs found in peripheral organs can also leave tissues via the blood stream. To study whether DCs derived from microglia and bone marrow can also use this route of emigration from the CNS, we performed a series of experiments in which we injected genetically labeled DCs into the striata of rats. We show here that these cells migrated from the injection site to the perivascular space, integrated into the endothelial lining of the CNS vasculature, and were then present in the lumen of CNS blood vessels days after the injection. Moreover, we also found these cells in both mesenteric lymph nodes and spleens. Hence, microglia- and bone marrow-derived DCs can leave the CNS via the blood stream.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2008 by the American Society for Investigative Pathology.